“…Because there were not significant differences on treatment x sampling time, only main effects of ruminal pH, ammonia-N and total VFA are shown in Table 1. Similar responses were found for supplementation of monensin (Fairfield et al, 2007), bentonite (Gulsen et al, 2000) or enzymes (Miller et al, 2008) in dairy cow diets.…”
“…Because there were not significant differences on treatment x sampling time, only main effects of ruminal pH, ammonia-N and total VFA are shown in Table 1. Similar responses were found for supplementation of monensin (Fairfield et al, 2007), bentonite (Gulsen et al, 2000) or enzymes (Miller et al, 2008) in dairy cow diets.…”
“…Since dry ewes were over-fed, they probably refused the unpalatable feed more easily than the lactating ewes. Moreover, enzyme supplementation may have modified the volatile fatty acids pattern in the rumen, as recently reported by Miller et al (2008), being responsible for the altering intake. Beauchemin et al (1995) also reported differences in the ration DM intake according to the enzyme dose in steers fed enzyme-treated forages, DM intake was reduced for medium doses whereas it increased for high and low doses.…”
Section: Fill Value Evaluation Experimentsmentioning
confidence: 77%
“…The use of enzymes in sheep diets has been limited to digestibility trials in wethers (Lee et al, 2000;PinosRodríguez et al, 2002) and to growth trials with fattening lambs (McAllister et al, 2000;Muwalla et al, 2007;Miller et al, 2008). As far as we know, however, only one experiment has been conducted with suckling sheep and goats (Titi and Lubbadeh, 2004).…”
Seventy-two multiparous ewes from two dairy breeds (Manchega, n 5 36 and Lacaune, n 5 36) were used in a replicated 2 3 2 factorial design to evaluate the effects of diet supplementation with an exogenous fibrolytic enzyme product on lactation performance and feed intake during the suckling period (weeks 1 to 4) according to breed. Ewes were blocked in groups of nine and fed ad libitum after lambing a diet based on 70% forage and 30% concentrate to which the enzyme was added after pelleting. Experimental concentrates were: control (without enzyme) and enzyme (fibrolytic enzyme complex, included at 0.47% volume to weight of concentrate). Twenty-four dry and open ewes (Manchega, n 5 12 and Lacaune, n 5 12) were also grouped by breed and used to measure the fill value of the ration used. During the suckling period, milk yield, milk composition, dry matter intake, lamb growth, as well as body weight change and body condition score change were not affected by enzyme supplementation. Breed effect was significant for milk yield, the Manchega ewes yielding less milk with a higher content of milk components than the Lacaune ewes. The opposite was observed for dry matter intake. Enzyme supplementation reduced intake by 9% in the dry ewes, resulting in a greater fill value of the diet. In conclusion, no lactational effects were detected when the fibrolytic enzyme product was added to the concentrate fed to dairy ewes.
“…There are also some inconsistencies on the effects of dose levels on ruminal fermentation kinetics. Some researches have shown that efficiency of forage utilisation was increased at the increasing dose levels of exogenous enzymes (Miller et al 2008) whereas others suggest that exogenous enzymes produced better results at a specific level, rather than showing a dose response (Jalilvand et al 2008).…”
The objective of this study was to evaluate the effects of three fibrolytic enzyme products (cellulase (CEL), xylanase (XYL) and a 1:1 mixture of CEL and XYL (MIX)) at three dose levels (0, 1 and 3 lL/0.5 g DM) on the in vitro fermentation of a diet for growing lambs. Bottles were incubated for 96 h at 39 C. A mathematical model was used to estimate the parameters describing the gas production (GP) curve (b, c and L). Dry matter degradability (DMD) and fibre (NDFD and ADFD) degradability were determined at the end of the incubation period. Metabolisable energy (ME) and short chain fatty acids (SCFA) were calculated at 24 h of incubation. The asymptotic GP (parameter b) was affected (p < 0.02) by enzyme product and dose level, with a significant linear response (p < 0.05). Dose level affected ME and SCFA with a significant linear (p < 0.05) and quadratic (p < 0.01) response. The interaction between enzyme product and dose level was significant (p < 0.05) for cumulative GP up to 72 and 96 h of incubation, pH, ADFD and DMD. The results suggest that application of exogenous cellulases has the potential to alter asymptotic GP and degradability of ADF and DM of a diet for growing lambs, but most of the results depend on the interaction between enzyme product and dose level. Future studies are required to determine the ideal combination between enzyme product and dose level for optimal degradation of ruminant feeds.
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