Effects of aluminum chloride on the kinetics of rat cortex synaptosomal ATP diphosphohydrolase 9EC 3.6.1.5)

Schetinger, Maria Rosa Chitolina; Wyse, Angela T. S.; Silva, Luis B. Da; Barcellos, Cláudia K.; Dias, Renato Dutra; Sarkis, João José Freitas

doi:10.1007/bf02785411

Cited by 12 publications

(9 citation statements)

References 24 publications

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“…Thus, as NTPDase and 5′‐nucleotidase enzymes are activated by Ca +2 or Mg 2+ cations, any alteration in the availability of ions affects the activities of these enzymes. Schetinger et al (1995), observed a competitive inhibition between aluminium and calcium, suggesting that the aluminium ion forms complexes with ATP and ADP, since these complexes are not hydrolyzed by nucleotidases.…”

Section: Discussionmentioning

confidence: 99%

“…NTPDase hydrolyzes the extracellular nucleoside tri and diphosphate in the presence of Ca 2+ or Mg 2+ , as it is activated by these cations (Zimmermann et al, 1998). Nucleotide hydrolysis may be impaired by Al, probably because it interferes in the homeostasis of divalent cations (Schetinger et al, 1995; Moretto et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have reported NTPDase activity in synaptosomes obtained from the hippocampus and cerebral cortex of rats (Battastini et al, 1991;Muller et al, 1993), such as in platelets (Pilla et al, 1996). In fact, Schetinger et al (1995) showed a significant inhibition of NTPDase activity by Al in vitro, observing a competitive inhibition with the divalent cation Ca 2+ . However, the in vivo effect of Al on NTPDase and 5 0 -nucleotidase, after a long-term exposure, have never been reported.…”

Section: Introductionmentioning

confidence: 99%

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The effect of aluminium on NTPDase and 5′‐nucleotidase activities from rat synaptosomes and platelets

Kaizer

Maldonado

Spanevello

et al. 2007

Intl J of Devlp Neuroscience

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Aluminium (Al), a neurotoxic compound, has been investigated in a large number of studies both in vivo and in vitro. In this study, we investigated the effect in vivo of long-term exposure to Al on NTPDase (nucleoside triphosphate diphosphohydrolase) and 5'-nucleotidase activities in the synaptosomes (obtained from the cerebral cortex and hippocampus) and platelets of rats. Here, we investigated a possible role of platelets as peripheral markers in rats. Rats were loaded by gavage with AlCl(3) 50 mg/(kg day), 5 days per week, totalizing 60 administrations. The animals were divided into four groups: (1) control (C), (2) 50 mg/kg of citrate solution (Ci), (3) 50 mg/kg of Al plus citrate (Al+Ci) solution and (4) 50 mg/kg of Al (Al). ATP hydrolysis was increased in the synaptosomes from the cerebral cortex by 42.9% for Al+Ci and 39.39% for Al, when compared to their respective control (p<0.05). ADP hydrolysis was increased by 13.15% for both Al and Al+Ci, and AMP hydrolysis increased by 32.7% for Al and 27.25% for Al+Ci (p<0.05). In hippocampal synaptosomes, the hydrolysis of ATP, ADP and AMP, was increased by 58.5%, 28.5% and 25.92%, respectively, for Al (p<0.05) and 36.7%, 22.5% and 37.64% for Al+Ci, both when compared to their respective controls. ATP, ADP and AMP hydrolysis, in platelets, was increased by 172.3%, 188.52% and 92.1%, respectively in Al+Ci, and 317.9%, 342.8% and 177.9%, respectively, for Al, when compared to their respective controls (p<0.05). Together, these results indicate that Al increases NTPDase and 5'-nucleotidase activities, in synaptosomal fractions and platelets. Thus, we suggest that platelets could be sensitive peripheral markers of Al toxicity of the central nervous system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of aluminium on NTPDase and 5′‐nucleotidase activities from rat synaptosomes and platelets

Kaizer

Maldonado

Spanevello

et al. 2007

Intl J of Devlp Neuroscience

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show abstract

“…A competitive inhibition between aluminium and Ca 2+ was suggested as the primary mechanism responsible for the inhibitory effect of aluminium on apyrase (EC 3.6.1.5; ecto-ATPDase) activity. Schetinger et al [241] observed that AlCl 3 inhibits both the ATPase and ADPase activities of synaptosomes isolated from rat cerebral cortex, with an estimated IC 50 value of 5.0 mM in the presence of 1.5 mM CaCl 2 . Here the enzyme activity remained below basal levels in the absence of Ca 2+ .…”

Section: Inactivation Of Neurotransmittersmentioning

confidence: 98%

Does neurotransmission impairment accompany aluminium neurotoxicity?

Gonçalves

Silva

2007

Journal of Inorganic Biochemistry

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“…However, recent data have raised serious questions about the hazards of this metal (1,2). Aluminum salts administered by different routes can produce toxic effects in animal models (3)(4)(5) some of which involve alterations in enzymatic activities (6,7). It is presently accepted that aluminum intoxication may induce anemia and encephalopathy in humans and animals (8).…”

Section: Introductionmentioning

confidence: 99%

Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice

Schetinger

Bonan

Morsch

et al. 1999

Braz J Med Biol Res

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The purpose of the present study was to investigate the in vitro and in vivo effects of aluminum sulfate on delta-aminolevulinic acid dehydratase (ALA-D) activity from the brain, liver and kidney of adult mice (Swiss albine). In vitro experiments showed that the aluminum sulfate concentration needed to inhibit the enzyme activity was 1.0-5.0 mM (N = 3) in brain, 4.0-5.0 mM (N = 3) in liver and 0.0-5.0 mM (N = 3) in kidney. The in vivo experiments were performed on three groups for one month: 1) control animals (N = 8); 2) animals treated with 1 g% (34 mM) sodium citrate (N = 8) and 3) animals treated with 1 g% (34 mM) sodium citrate plus 3.3 g% (49.5 mM) aluminum sulfate (N = 8). Exposure to aluminum sulfate in drinking water inhibited ALA-D activity in kidney (23.3 ± 3.7%, mean ± SEM, P<0.05 compared to control), but enhanced it in liver (31.2 ± 15.0%, mean ± SEM, P<0.05). The concentrations of aluminum in the brain, liver and kidney of adult mice were determined by graphite furnace atomic absorption spectrometry. The aluminum concentrations increased significantly in the liver (527 ± 3.9%, mean ± SEM, P<0.05) and kidney (283 ± 1.7%, mean ± SEM, P<0.05) but did not change in the brain of aluminum-exposed mice. One of the most important and striking observations was the increase in hepatic aluminum concentration in the mice treated only with 1 g% sodium citrate (34 mM) (217 ± 1.5%, mean ± SEM, P<0.05 compared to control). These results show that aluminum interferes with delta-aminolevulinate dehydratase activity in vitro and in vivo. The accumulation of this element was in the order: liver > kidney > brain. Furthermore, aluminum had only inhibitory properties in vitro, while in vivo it inhibited or stimulated the enzyme depending on the organ studied.

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Effects of aluminum chloride on the kinetics of rat cortex synaptosomal ATP diphosphohydrolase 9EC 3.6.1.5)

Cited by 12 publications

References 24 publications

The effect of aluminium on NTPDase and 5′‐nucleotidase activities from rat synaptosomes and platelets

The effect of aluminium on NTPDase and 5′‐nucleotidase activities from rat synaptosomes and platelets

Does neurotransmission impairment accompany aluminium neurotoxicity?

Effects of aluminum sulfate on delta-aminolevulinate dehydratase from kidney, brain, and liver of adult mice

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