Effects of .ALPHA.-chlorohydrin on rat sperm motions in relation to male reproductive functions.

Ban, Yoshiki; Asanabe, U.; Inagaki, Satoru; Sasaki, Masahiro; Nakatsuka, Toshio; Matsumoto, Hajime

doi:10.2131/jts.24.5_407

Cited by 14 publications

(26 citation statements)

References 12 publications

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“…However, the use of computer-assisted sperm analysis made it possible to practically and objectively evaluate the quality of sperm motility. These results made it easy to predict that the non-progressive sperm in the ACH dose groups would not reach the oviduct, which Ban et al (1999) had reported. Thus it appeared more obvious that linearity parameters (VSL, STR and LIN) observed only in the ACH groups provided the most accurate prediction of abnormal sperm motion which was strongly associated with the reduced fertility index or implantation loss, even if sperm had highly vigorous movement.…”

Section: Supplemental Analysesmentioning

confidence: 57%

“…Kato et al (2001) conducted collaborative studies to determine the utility and sensitivity of nine sperm motion parameters generated by a HTM-IVOS and reported decreases in percentage of motile sperm, percentage of progressive sperm, VAP, VSL, VCL, ALH, STR and LIN, and an increase in BCF was observed when rats were given a single dose of 10, 15, 17.5 or 20 mg ACH/kg. Ban et al (1999) reported that VCL and ALH decreased when 3 mg ACH/kg was administered for 9 days to rats and sperm motion was analyzed with the CellSoft-CASA TM 4000 series system (CRYO Resources, Ltd., NY, USA) and that a low pregnancy rate was achieved. Their findings suggested that the reduction in sperm number reaching the oviducts after mating resulted in only a rare chance to fertilize (Ban et al, 1999).…”

Section: Effect Of Achmentioning

confidence: 99%

“…Ban et al (1999) reported that VCL and ALH decreased when 3 mg ACH/kg was administered for 9 days to rats and sperm motion was analyzed with the CellSoft-CASA TM 4000 series system (CRYO Resources, Ltd., NY, USA) and that a low pregnancy rate was achieved. Their findings suggested that the reduction in sperm number reaching the oviducts after mating resulted in only a rare chance to fertilize (Ban et al, 1999). Using a standard scoring system (manual method), Woods and Garside (1996) noted decreased sperm motility and abnormal movements of sperm from male rats treated with 5 mg ACH/ kg for at least 5 consecutive days, and none of the females mated with the treated males became pregnant.…”

Section: Effect Of Achmentioning

confidence: 99%

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Key Parameters of Sperm Motion in Relation to Male Fertility

et al. 2004

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-This study was undertaken to detect key parameters of rat sperm motion in relation to male fertility by comparing the differences in sperm motion induced by treatment with α-chlorohydrin (ACH), known to produce spermatotoxicity, and nitrobenzene (NTB), known to produce testicular toxicity. Male rats received ACH (5 or 20 mg/kg/day) or NTB (60 mg/kg/day) for either 3 days or 18 days. Epididymal sperm was assessed for motility using a Hamilton-Thorne Sperm Analyzer (HTM-IVOS). Numerical data for statistical analysis and graphical renditions of sperm motion using parameters in radar charts and reconstructed sperm tracks were analyzed to evaluate sperm motion. Males were allowed to copulate with untreated females and cesarean sections were conducted in order to examine the effects of drug administration on male fertility. Linearity of sperm track (linearity (LIN) and/or straightness (STR)) decreased and/or beat cross frequency (BCF) increased only in ACH groups (5 or 20 mg/kg/day), although the percentage of motile sperm, sperm velocities (average path velocity (VAP), curvilinear (VCL), and straight line velocity (VSL)) and amplitude of lateral head displacement (ALH) decreased on Day 18 in both ACH and NTB (60 mg/kg/day) groups. Furthermore, from the individual reconstructed sperm tracks, it was clear that ACH-treated spermatozoa were characterized by abnormal motion ("jerking") with low vigor (low velocities) and little or no forward progression. Finally, only ACH treatment led to a reduction in pregnancy rate or infertility. Therefore, our results suggest that linearity (especially VSL, STR and LIN) in sperm motion is a key parameter for assessing a chemical's potential to induce male infertility.

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Section: Supplemental Analysesmentioning

confidence: 57%

Section: Effect Of Achmentioning

confidence: 99%

Section: Effect Of Achmentioning

confidence: 99%

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Key Parameters of Sperm Motion in Relation to Male Fertility

et al. 2004

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“…Meanwhile, we demonstrated that impaired fertility of male rats treated with -chlorohydrin was indicated by reduction of the percentage of motile sperm and sperm velocity (Kato et al, 1999). Ban et al (1999) also reported that -chlorohydrin-impaired male fertility was caused by a decreased number of sperms reaching the oviducts after mating due to decreases in the percentage of motile sperm, sperm velocity and ALH. These findings suggest that interaction with decreased number of sperms and suppressed sperm motion caused impaired fertility of the males treated with ADR.…”

Section: Discussionmentioning

confidence: 96%

Sperm motion analysis in rats treated with adriamycin and its applicability to male reproductive toxicity studies.

Kato¹,

Makino²,

Kimura³

et al. 2001

J. Toxicol. Sci.

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Adriamycin (ADR), an anticancer drug which induces testicular toxicity, was administrated to Slc:SD male rats at doses of 0.5, 1.0, and 2.0 mg/kg intravenously once a week for 4 weeks. The males treated with ADR were mated with untreated females, and sperm analyses (motion, count, and morphology) were performed. Sperm motion was analyzed by Hamilton-Thorne Sperm analyzer (HTM-IVOS) to investigate the useful parameters. Copulated females were necropsied at Day 13 of gestation, and reproductive status was evaluated.In ADR-treated groups, the testicular weight was dose-dependently decreased. Associated with this decrease was a depletion of the number of spermatogonia noted histopathologically at all dosage levels. Sperm morphological abnormalities, which were classified as tailless sperm and/or no-hook head sperm, were increased in both the 1.0 and 2.0 mg/kg groups. The males treated with ADR at 1.0 and 2.0 mg/kg had a decreased number of sperms per cauda epididymis. In sperm motion analysis, decreases in the percentage of motile sperm, percentage of progressive sperm, and sperm velocity (straight line velocity and curvilinear velocity) were noted at 2.0 mg/kg. Impaired fertility was noted at 2.0 mg/kg in the form of decreased numbers of implantations and live embryos, and an increased number of pre-implantation losses.In conclusion, ADR induced deterioration of sperm motion and sperm content, which were responsible for the adverse effect on male fertility. The most sensitive indicators to detect male reproductive toxicity induced by ADR were testicular weight and histopathological findings in the testis. Among the parameters generated by HTM-IVOS, the percentage of motile sperm, the percentage of progressive sperm, and sperm velocity are useful for assessing male fertility.

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“…The low-dose effect is noted in mature sperm contained in the cauda epididymis and vas deferens without visible morphologic changes, and is known to inhibit the glycolytic pathway [13][14][15]. When the movement of spermatozoa from male rats treated with α-chlorohydrin was analyzed with a computer assisted sperm analysis (CASA) system, swimming velocity was reduced with or without a decrease in the percentage of motile sperm [16,17]. The number of implantations was also decreased with suppressing their progressive motility [17] but the reason for causing fertility disorder is unclear.…”

mentioning

confidence: 99%

In Vitro Evaluation of Acrosomal Status and Motility in Rat Epididymal Spermatozoa Treated with .ALPHA.-Chlorohydrin for Predicting Their Fertilizing Capacity.

Kato¹,

Makino²,

Kimura³

et al. 2002

Journal of Reproduction and Development

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Abstract. Rat cauda epididymal spermatozoa treated with α-chlorohydrin at concentrations of 0, 0.01, 0.1, and 1.0 under conditions that support in vitro fertilization were used to evaluate the effects of acrosomal status and sperm motility in predicting their fertilizing capacity. Acrosomal status was assessed by fluorescein isothiocyanate-conjugated concanavalin A (FITC-ConA) lectin assay in combination with supravital stain using calcein acetoxy methyl ester (CAM) and ethidium homodimer-1 (EthD-1) at 1, 3, and 5 h of incubation. Sperm motility was also examined with a computer assisted sperm analysis (CASA) system at the same time points as acrosomal status. The movement of spermatozoa treated with α-chlorohydrin at 0.01 mM was similar to the control spermatozoa over 5 h of incubation. The 0.1 mM treated spermatozoa showed progressive movement at 1 h of incubation, and low vigorous movement was seen after 3 h of incubation. The 1.0 mM treated spermatozoa showed low progression and low vigorous movement over 5 h of incubation. The FITCConA patterns of rat spermatozoa undergoing acrosome reaction were marked by the appearance of bright green fluorescence from the acrosomal region on their head, and were clearly distinguished from the degenerative acrosome loss in dead sperm. A time-related increase in the percentage of live acrosome lost sperm was observed in the control and 0.01 mM α-chlorohydrin treated groups, but a low percentage and no time-related increase in live acrosome lost sperm without degenerative acrosome loss in dead sperm were observed in the 0.1 and 1.0 mM treated groups. On the basis of these results, rat spermatozoa labeled with FITC-ConA combined with CAM and EthD-1 can have their acrosomal status clearly distinguished. The α-chlorohydrin treated spermatozoa were suppressed not only vigorous movement but also acrosome reaction, and the results suggested that the present staining procedure for acrosomal status can be a useful indicator for predicting spermatozoal fertilizing capacity.

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Effects of .ALPHA.-chlorohydrin on rat sperm motions in relation to male reproductive functions.

Cited by 14 publications

References 12 publications

Key Parameters of Sperm Motion in Relation to Male Fertility

Key Parameters of Sperm Motion in Relation to Male Fertility

Sperm motion analysis in rats treated with adriamycin and its applicability to male reproductive toxicity studies.

In Vitro Evaluation of Acrosomal Status and Motility in Rat Epididymal Spermatozoa Treated with .ALPHA.-Chlorohydrin for Predicting Their Fertilizing Capacity.

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