Effects of Allium hookeri Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells

Abstract

Oxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the Allium hookeri extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it was found that a non-cytotoxic concentration of the extracts increased the viability and size of the human DPC spheroid, which was associated with the increased expression of hair-growth-related genes in cells. To determine whether or not these effects could be attributed to intracellular anti-oxidative effects, liquid chromatography-mass spectrometry alongside various biochemical analyses are conducted herein. An ingredient called alliin was identified as one of the main components. Furthermore, AHE treatment induced a significant decrease in H2O2-mediated cytotoxicities, cell death, and cellular senescence in human DPCs. Upon analyzing these results with a molecular mechanism approach, it was shown that AHE treatment increased β-Catenin and NRF2 translocation into the nucleus while inhibiting the translocation of NF-κB (p50) through p38 and PKA-mediated phosphorylations of GSK3β, an upstream regulator of those proteins. These results overall indicate the possibility that AHE can regulate GSK3β-mediated β-Catenin, NRF2, and NF-κB signaling to enhance hair-inductive properties and ultimately protect against oxidative stress-induced cellular damage in human DPCs.

Phloroglucinol Enhances Anagen Signaling and Alleviates H2O2-Induced Oxidative Stress in Human Dermal Papilla Cells

“…The efficacy of PG in inhibiting intracellular ROS level was assessed using 2’,7’-dichlorofluorescein diacetate (H 2 DCFDA; #D6883; Sigma-Aldrich; Merck KGaA), following the method described by Ng in 2021 [ 39 , 40 ]. HDPCs (5 × 10 3 ) were seeded in a 96-well fluorescence microtiter plate and incubated at 37°C for 24 h. Subsequently, the cells were treated with various concentrations of PG (0-10 μM) for 60 min.…”

“…The TOPFlash luciferase reporter assay was conducted following the method described by S. Park et al . [ 40 ]. 293T cells (5 × 10 5 ) were seeded in 6-well plates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA) using Lipofectamine transfection reagent (#18324012; Invitrogen; Thermo Fisher Scientific).…”

Dissecting hair breakage in alopecia areata: the central role of dysregulated cysteine homeostasis

“…This leads to an elevation in free zinc concentration, subsequently activating transcription factors such as nuclear factor erythroid 2-related factor 2 (Nrf2) (Ge et al 2021 ; Qin et al 2021 ). These factors play a central role in the hair follicle cellular antioxidant response, orchestrating the transcription of various antioxidative enzyme genes (Peterle et al 2023 ; Park et al 2023 ). Such enzymes mitigate harmful free radicals, fortifying cells against oxidative insults.…”

Anti-Melanogenic Effects of Lilium lancifolium Root Extract via Downregulation of PKA/CREB and MAPK/CREB Signaling Pathways in B16F10 Cells

“…The root of Lilium lancifolium utilized in this study was obtained from a cultivation site located in the Pyeongchang region (Gangwon-do, Republic of Korea) in May 2022. The extraction method of L. lancifolium root was performed according to the previously described method [100]. After three cycles of rinsing with distilled water, the plants were left to air dry at room temperature.…”

“…HPLC-HRMS analysis was conducted following a previously described method [100]. The chemical profiles of LRE were examined using a Thermo Ultimate-3000 UPLC system (Thermo Fisher Scientific, Waltham, MA, USA) coupled with Thermo LTQ-Orbitrap XL (Thermo Fisher Scientific) and executed utilizing an ACQUITY BEH C18 column (1.7 µm, 150 mm × 2.1 mm).…”