Protein can be extracted effectively from excess sludge by thermal alkaline process. During the extraction process, insoluble protein in sludge was turned into soluble protein, and part of the soluble protein was hydrolyzed into peptide. Then part of the soluble protein and peptide were degraded into amino acids, generating by-products: water, carbon dioxide and ammonium. Therefore, to obtain the maximum concentration of soluble protein, it is necessary to establish a kinetic model describing the process of sludge protein extraction. The report indicated that the extraction mechanism of sludge protein was according to first-order continuous reaction kinetic. A kinetic model of the reaction was established to explain the relationship between the rate constant, temperature and hydrolysis time of the first-order reaction. The optimum conditions were as follows: 0.1 M NaOH, reaction temperature 70°C, hydrolysis time 4 h. Protein separated and obtained from sludge hydrolysate by centrifugation and filtration, which could be used as a feed additive or foaming agent.