Effects of Albendazole Sulphoxide on Embryonic, Foetal and Placental Parameters in Wistar Rats

Abstract

SUMMARY:The aim of this work was to evaluate the effect of albendazole sulphoxide (ABZSO), administered to Wistar rats during pregnancy on embryonic, foetal and placental parameters. A colpocytological control was performed daily and detection of spermatozoa in the vaginal smear was considered as day 0 of pregnancy. For the preimplantational study, ABZSO (10 mg/kg) was orally administered at day 2 of pregnancy; at day 4 the embryos were collected. For the postimplantational study, ABZSO (10 mg/kg) was orally administered by gavages at day 2, 6 or 10 of pregnancy (G2, G6 and G10 Groups respectively); the control group was administered the same volume of carboxymethylcellulose vehicle used to prepare the drug suspension. Fetuses were obtained from pregnant rats sacrificed on day 20 of gestation. Maternal body weight gains were analyzed using the one-way ANOVA test. Embryonic and foetal variables were analized on a per litter basis by the Kruskal-Wallis test. Skeletal anomalies were analyzed using an X 2 test. The significance level accepted was established at P<0.05. In the preimplantational analysis, the cleavage rate was lower in the experimental group (P<0.05). In the postimplantational analysis there were no differences in the net weight increase among females of the different groups (P>0.05). The number of fetuses and the foetal vesicles weight were lower in the G10 group (P<0.05). This group showed the highest percentage of resorptions (P<0.05) and fetuses morphologically abnormal. An increase in the number of bones affected in fetuses of G6 and G10 groups was observed. The most common malformations were at vertebral, costal and head level. Weights and placental diameters were lower in the G10 group (P<0.05). We conclude that ABZSO at the dose used in this study affects the cleavage rate in preimplantational embryo development, without interrupting pregnancy. Furthermore; the developmental toxicity is related to day of administration.

Albendazole treatment in laying hens: Egg residues and its effects on fertility and hatchability

“…The embryotoxic and teratogenic effects of ABZ when administered to pregnant animals have been demonstrated. ABZ has been shown to produce increased resorption, decreased birth weight, teratogenic effects, such as external, skeletal and vascular abnormalities, when administered during gestation in mice, rats, rabbits, sheep and zebrafish (Capece et al., ; Carlsson, Patring, Erik Ullerås, & Oskarsson, ; Cristòfol et al., ; Navarro, Cristòfol, Carretero, Arboix, & Ruberte, ; Teruel, Felipe, Solana, Sallovitz, & Lanusse, ; Teruel et al., ). Most of the ABZ toxicity studies have been carried out in mammals, with few data in birds.…”

“…However, ABZ is currently being extra‐label used in avian production systems. Toxicological studies in both farm and laboratory animals have shown ABZ and its active sulphoxide metabolite (ABZSO) to be embryotoxic/teratogenic (Delatour, Garnier, Benoit, & Longin, ; Teruel, García, & Catalano, ). The pharmacokinetic behaviour and tissue residue depletion of these anthelmintics have been well reported in ruminant species.…”

Pharmacodynamics Studies on Ricobendazole In Male Rats

“…The second group was given 7.5 mg/kg bwt of ricobendazole subcutaneous injection once. These doses were calculated according to oral dose recorded by Teruel et al, (2009). The third group was given 7.5 mg/kg bwt of ricobendazole subcutaneous injection, twice with 3 week intervals.…”

Poster Communications

“…Although flubendazole is the only BZD approved for parasite control in poultry, albendazole (ABZ) is frequently extra-label used for this purpose in avian species. Toxicological studies in both farm and laboratory animals have shown ABZ and its active sulphoxide metabolite (ABZSO) to be embryotoxic/teratogenic (Delatour et al, 1984, Teruel et al, 2009). The goal of the work reported here was to evaluate the effect of ABZ treatment on the fertility and hatchability of eggs collected from treated breeder hens.…”