Albendazole treatment in laying hens: Egg residues and its effects on fertility and hatchability

Abstract

This work characterized the egg residual concentrations of albendazole (ABZ) and its sulphoxide (ABZSO) and sulphone (ABZSO ) metabolites and evaluated their effect on egg fertility and hatchability after ABZ treatments to laying hens. Seventy hens were allocated in groups: Group-1 was the control without treatment; Group-2 received a single ABZ oral dose (10 mg/kg); Group-3, -4 and -5 were treated with ABZ in medicated feed over 7 days at 10, 40, or 80 mg kg day , respectively. Eggs were analyzed to determine the ABZ/metabolite level by HPLC or subjected to incubation to evaluate the fertility and hatchability. Only ABZSO and ABZSO metabolites were quantified in egg after ABZ single oral administration with maximum concentrations of 0.47 ± 0.08 and 0.30 ± 0.07 μg/ml, respectively. ABZ and its metabolites were found in eggs after 7-day ABZ treatments. The egg residue exposure estimated as AUCs (areas under the concentration vs. time curve) were 100.5 (ABZ), 56.3 (ABZSO) and 141.3 μg hr g (ABZSO ). ABZ administration did not affect the egg fertility at any dosages. Egg hatchability was not affected by ABZ treatment at 10 mg/kg in medicated feed, but it decreased when the dose was 4-8 times higher. These results should be considered when ABZ is used for deworming laying hens.

Oral and topical extra‐label administration of fipronil to laying hens: Assessment of the egg residue patterns

“…Taking into account, the physiology of egg formation allowed us to understand why high fipronil residues were found in the egg. Like other described drugs (Donoghue et al, 1997;Marmulak et al, 2015;Moreno et al, 2018), fipronil administered to laying hens by both routes is absorbed and it reaches the ovary, follicles and oviduct. The follicles go through three phases until they become eggs.…”

Separation and Detection of Abamectin, Ivermectin, Albendazole and Three Metabolites in Eggs Using Reversed-Phase HPLC Coupled with a Photo Diode Array Detector

“…Zebrafish embryo exposure experiments confirmed that the developmental toxicity and teratogenic effects were displayed by ABZ itself, rather than its first metabolite albendazole sulfoxide (ABZSO 2 ) and the subsequent main metabolites albendazole sulfone (ABZSO) and albendazole-2-aminosulfone (ABZSO 2 NH 2 ) [ 7 ]. It has been proven that egg hatchability was decreased when the dose of ABZ in medicated feed was 40–80 mg/kg [ 8 ]. Residues in foods of animal origin at low concentrations are unlikely to lead directly to the toxic effects described above.…”