Effects of aflatoxin B1 and T-2 toxin on the granulocyte-macrophage progenitor cells in mouse bone marrow cultures

Dugyala, Raviprakash R.; Kim, Yang-Won; Sharma, Raghubir P.

doi:10.1016/0162-3109(94)90007-8

Cited by 26 publications

(7 citation statements)

References 24 publications

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“…At the end of this study, the examined serum samples taken from group treated with aflatoxin and zeocem, and group treated with aflatoxin alone expressed highly significant reduction of TNF-α release. In parallel to this respect (Dugyala et al, 1994); (Adrian et al, 1998) had demonstrated that inhibitory effects of aflatoxin on macrophage mediators could be a result of suppressed proliferation of the granulocyte-macrophage(GM) progenitor cells to granulocyte, macrophage and GMcolonies which primes macrophage to release proinflamatory mediators including IL (1, 6,10) and TNF-α. Hence, co-treatment of nutritox with aflatoxin appears to enhance the production of TNF-α because the ability of the included probiotic bacteria (Lactobacillus strains) to bind with aflatoxin (Peltonen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Effects of Chemical and Biological Anti-mycotoxins on Performance, Haematological, Biochemical and Immunological Parameters of Broiler Chickens during Aflatoxicosis

Kilany

Helmi

Fares

et al. 2020

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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The journal of Toxicology and pest control is one of the series issued twice by the Egyptian Academic Journal of Biological Sciences, and is devoted to publication of original papers related to the interaction between insects and their environment. The goal of the journal is to advance the scientific understanding of mechanisms of toxicity. Emphasis will be placed on toxic effects observed at relevant exposures, which have direct impact on safety evaluation and risk assessment. The journal therefore welcomes papers on biology ranging from molecular and cell biology, biochemistry and physiology to ecology and environment, also systematics, microbiology, toxicology, hydrobiology, radiobiology and biotechnology. www.eajbs.eg.net

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Section: Discussionmentioning

confidence: 99%

Effects of Chemical and Biological Anti-mycotoxins on Performance, Haematological, Biochemical and Immunological Parameters of Broiler Chickens during Aflatoxicosis

Kilany

Helmi

Fares

et al. 2020

Egyptian Academic Journal of Biological Sciences, F. Toxicology

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“…In several cell lines, inhibiting DNA and RNA synthesis and toxic effects involving the cell membrane and cell proliferation were found (Dugyala et al 1994;Lautraite et al 1995;ParentMassin 2004). T-2 toxin induced apoptotic cell death of lymphocytes in the thymus, splenic white pulp (Shinozuka Abstract T-2 toxin is one of the type A trichothecene mycotoxins that is considered to be the most toxic of the trichothecenes.…”

Section: Introductionmentioning

confidence: 96%

Toxic effects of HT-2 toxin on mouse oocytes and its possible mechanisms

et al. 2015

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T-2 toxin is one of the type A trichothecene mycotoxins that is considered to be the most toxic of the trichothecenes. T-2 toxin has been shown to exert various toxic effects in farm animals and humans, as it induces lesions in the brain and in lymphoid, hematopoietic, and gastrointestinal tissues. HT-2 toxin is the major metabolite of T-2 toxin. There is little information regarding the effects of HT-2 toxin on the female reproductive system, particularly oocyte maturation. Thus, in this study, we investigated the toxic effects of HT-2 on mouse oocyte maturation and its possible mechanisms of action. HT-2 toxin exposure disrupted oocyte maturation, reduced actin expression in both the oocyte cortex and cytoplasm, and disrupted meiotic spindle morphology by reducing p-MAPK protein level. HT-2 toxin exposure also induced oxidative stress and resulted in oocyte apoptosis, as shown by ROS accumulation, increased SOD mRNA level, and the expression of the early apoptosis marker Annexin V and increased caspase-3 and bax mRNA levels. Additionally, HT-2 toxin exposure increased LC3 and ATG12 protein levels and lc3 and atg14 mRNA levels, which indicated that HT-2 toxin induced autophagy in mouse oocytes. We also examined for possible epigenetic modifications. Fluorescence intensity analysis showed that 5mC level increased after HT-2 toxin exposure, whereas H3K9me2 and H3K27me3 levels decreased after HT-2 toxin exposure, which indicated that DNA and histone methylations were altered. Thus, our results indicated that HT-2 toxin exposure reduced mouse oocyte maturation capability by affecting cytoskeletal dynamics, apoptosis/autophagy, oxidative stress, and epigenetic modifications.

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“…Several studies showed that it also inhibits the mitochondrial electron transport system, mitochondrial function, mitochondrial protein synthesis, augmented lipid peroxidation 5 8 . Cell membrane disruption and toxic effect of cell proliferation and cell division were found in several cell lines with T-2 toxin exposure 1 9 . Multiorgan effects including emesis, diarrhea, weight loss, nervous disorders, cardiovascular alteration, immune suppression, hemostatic derangements, skin toxicity and bone marrow damage are also caused by T-2 toxin 10 .…”

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confidence: 99%

Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes

Zhang

Han

Zhu

et al. 2016

Sci Rep

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T-2 toxin is a main type A trichothecene mycotoxin which is the most toxic trichothecence. T-2 toxin has posed various toxic effects on human and animals in vigorous cell proliferation tissues like lymphoid, hematopoietic and gastrointestinal tissues, while HT-2 toxin is the major metabolite which is deacetylated by T-2 toxin. In this study, we focused on the toxic effects of HT-2 on porcine oocyte maturation. We treated the porcine oocyte with HT-2 toxin in vitro, and we first found that HT-2 treatment inhibited porcine oocyte polar body extrusion and cumulus cell expansion. We observed the disrupted meiotic spindle morphology after treatment, which might be due to the reduced p-MAPK protein level. Actin distribution was also disturbed, indicating that HT-2 affects cytoskeleton of porcine oocytes. We next explored the causes for the failure of oocyte maturation after HT-2 treatment. We found that HT-2 treated oocytes showed the increased ROS level, which indicated that oxidative stress had occurred. We also detected autophagy as well as early apoptosis in the treatment oocytes. Due to the fact that oxidative stress could induced apoptosis, our results indicated that HT-2 toxin caused oxidative stress induced apoptosis and autophagy, which further affected porcine oocyte maturation.

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Effects of aflatoxin B1 and T-2 toxin on the granulocyte-macrophage progenitor cells in mouse bone marrow cultures

Cited by 26 publications

References 24 publications

Effects of Chemical and Biological Anti-mycotoxins on Performance, Haematological, Biochemical and Immunological Parameters of Broiler Chickens during Aflatoxicosis

Effects of Chemical and Biological Anti-mycotoxins on Performance, Haematological, Biochemical and Immunological Parameters of Broiler Chickens during Aflatoxicosis

Toxic effects of HT-2 toxin on mouse oocytes and its possible mechanisms

Exposure to HT-2 toxin causes oxidative stress induced apoptosis/autophagy in porcine oocytes

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