Effects of Aerobic and Anaerobic Incubation On the Quality of Ram Semen

Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage

“…(2008), which underscored the durability of stallion sperm quality at 4°C under anaerobic conditions using a milk‐based extender. This accord is further substantiated by existing observations regarding the critical role of gaseous environments in sustaining sperm viability, particularly within lower temperature ranges (Samo et al., 2006).…”

“…This was appropriately demonstrated in our study, where anaerobic preservation at 4°C preserved semen quality for 42 h. Additionally, at these lower temperatures, spermatozoa exhibit decreased metabolic activity, resulting in reduced oxygen demand and CO 2 release. This not only prevents toxic accumulation but also stabilizes pH, further contributing to the prolonged preservation of semen quality under anaerobic conditions (Samo et al., 2006). However, even with these findings, it is essential to conduct additional tests to measure the concentration of CO2 and pH accurately to ensure comprehensive semen quality assessment and preservation.…”

“…While antioxidants can ameliorate oxidative stress (Khalil et al., 2023), their excessive supplementation may disrupt the finely‐tuned redox balance within spermatozoa, potentially delaying their functionality and motility (Ben Moula et al., 2023). By means of opting for anaerobic conditions, which mitigate oxidative stress at its source, the delicate equilibrium required for successful artificial insemination can be maintained more effectively (Samo et al., 2006). Given this context, our study aims to assess the sperm quality of INRA180 rams stored in a skim milk extender at 4°C for 48 h, under aerobic and anaerobic conditions.…”