Effects of adipose derived stromal vascular fraction on diabetic neuropathy: an experimental study

Karatan, Berrak; Akşam, Ersin; Erden, Esra; Demirseren, Mustafa Erol

doi:10.1080/2000656x.2019.1632205

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…The clinical improvement result of this study is in accordance with other studies, that freshly harvested SVF containing large numbers of CD34+ cells injected to mouse ischemic hind limb, was associated with an increase in the blood flow and the capillary density and an incorporation of the cells in the leg vasculature [17]. A study by Karatan et al found that myelin sheath becomes thicker after SVF injection [18].…”

Section: Plos Neglected Tropical Diseasessupporting

confidence: 91%

Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report

et al. 2023

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Background Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of mononuclear cells, progenitor cells and about 1–10% are mesenchymal stromal cells. These cells are an ideal candidate for regenerative medicine for peripheral neuropathy. Leprosy is a disabling disorder with neuropathy, usually with consequences of permanent disability of the extremities. We conducted a preliminary study to evaluate the cell yield, its characteristics and clinical outcomes after SVF injections in four leprosy patients. Methods Four post leprosy patients were recruited and evaluated for sensory testing (warm detection, cold detection, vibration, pain and sensation) on the ulnar area of the hand. Liposuction was done and adipose tissue was processed into SVF with a closed system and injected to the ulnar area of the hand at the dorsal and palmar side. Evaluation of sensory testing was done after 3 days, 1 week, 1 month and 3 months following SVF injection. SVF was also characterized using flow cytometry, cell counting, sterility and presence of mycobacteria. Results The results showed that leprosy patients had a low count of mesenchymal cells and a high amount of CD34/CD45 positive cells. One patient was positive for mycobacteria from his adipose tissue and SVF. Sensory examination after SVF injection showed an improvement in temperature and pain sensation in the palmar and superficial branch. Meanwhile, touch sensation improved on the dorsal branch, and there was no improvement for vibration in all patients. Conclusions The results showed that SVF had a potential to improve sensory loss in leprosy patients.

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Section: Plos Neglected Tropical Diseasessupporting

confidence: 91%

Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report

et al. 2023

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“…Compared to ADSC, the preparation of SVF is even simpler, with single protease digestion yielding 2–6 × 10 6 SVF cells (CSVF) per 1 mL of fat with ≥90% cell viability. Many studies have shown that ADSC and CSVF can promote tissue repairs, such as skeletal tissue [ 28 , 29 , 30 , 31 ], muscle tissue [ 32 , 33 , 34 ], nerve damage [ 35 , 36 , 37 , 38 ], and cardiovascular injury [ 39 , 40 , 41 ]. Meanwhile, the tissue source of ADSC and CSVF is widely available, and a large amount of adipose tissue can be obtained for ADSC and CSVF extraction during liposuction, manual liposuction, etc., while the invasive procedure in obtaining bone marrow MSC and muscle-derived stem cells have limited their application in clinic.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

Chen

et al. 2022

Bioengineering

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Background: Anal sphincter incontinence (ASI) can cause a serious decline in the quality of life and can cause a socioeconomic burden. Studies have shown that bone marrow mesenchymal stem cells (MSC) have significant therapeutic effects on ASI, but the cost and risk of MSC harvest limit their further application. In contrast, adipose tissue derived stem cells (ADSC) and cellular stromal vascular fraction (CSVF) as stem cell sources have multipotency and the advantage of easy harvest. Objective: Here we aim to investigate the effects of ADSC and CSVF on treating ASI and compare them to that of bone marrow MSC. Methods: Bone marrow MSC, ADSC, and CSVF were obtained and labeled with green fluorescent protein (GFP), and CSVF was labeled with DIL. Sprague Dawley (SD) rats were divided into 5 groups. Four groups were injected with 0.2 mL phosphate buffer saline (PBS), 1 × 107/0.2 mL of MSC, ADSC, or CSVF, respectively, after model establishment. The control group received no treatment. The repair was assessed by anal functional tests and immunostaining on day 5 and day 10 after injection. Results: MSC, ADSC, and CSVF significantly promoted tissue repair and the recovery of muscle contraction and electromyographic activity in ASI. The generation of myosatellite cells by injected MSC, ADSC, and CSVF was found in the wounded area. On day 5, CSVF showed highest therapeutic effect, while on day 10, MSC and ADSC showed higher therapeutic effects than CSVF. When comparing the effects of MSC and ADSC, ADSC was slightly better than MSC in the indexes of anal pressure, etc. Conclusion: ADSC and CVSF are alternative stem cell sources for ASI repair.

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Stem cells as a regenerative medicine approach in treatment of microvascular diabetic complications

Poojari,

Wairkar,

Kulkarni

2023

Tissue and Cell

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Effects of adipose derived stromal vascular fraction on diabetic neuropathy: an experimental study

Cited by 3 publications

References 14 publications

Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report

Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

Stem cells as a regenerative medicine approach in treatment of microvascular diabetic complications

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