Effect of adipose derived stromal vascular fraction on leprosy neuropathy: A Preliminary report

Abstract

Background Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of mononuclear cells, progenitor cells and about 1–10% are mesenchymal stromal cells. These cells are an ideal candidate for regenerative medicine for peripheral neuropathy. Leprosy is a disabling disorder with neuropathy, usually with consequences of permanent disability of the extremities. We conducted a preliminary study to evaluate the cell yield, its characteristics and clinical outcomes after SVF injections in four leprosy patients. Methods Four post leprosy patients were recruited and evaluated for sensory testing (warm detection, cold detection, vibration, pain and sensation) on the ulnar area of the hand. Liposuction was done and adipose tissue was processed into SVF with a closed system and injected to the ulnar area of the hand at the dorsal and palmar side. Evaluation of sensory testing was done after 3 days, 1 week, 1 month and 3 months following SVF injection. SVF was also characterized using flow cytometry, cell counting, sterility and presence of mycobacteria. Results The results showed that leprosy patients had a low count of mesenchymal cells and a high amount of CD34/CD45 positive cells. One patient was positive for mycobacteria from his adipose tissue and SVF. Sensory examination after SVF injection showed an improvement in temperature and pain sensation in the palmar and superficial branch. Meanwhile, touch sensation improved on the dorsal branch, and there was no improvement for vibration in all patients. Conclusions The results showed that SVF had a potential to improve sensory loss in leprosy patients.

Pluripotent stem cells: Basic biology or else differentiations aimed at translational research and the role of flow cytometry

“…Moreover, it is technically advisable to use homogenous populations of somatic cells or progenitor cells to reprogram them into iPSCs for robustness in downstream applications. For example Adipose-derived stromal vascular fractions (SVF) are a heterogeneous population containing progenitor cells, mononuclear cells and 1%-10% mesenchymal stem cells, adipose-derived endothelial cells and pericytes [23]. Precisely, the SVFs contain adipose-derived stem cells and hematopoietic stem cell progenitors expressing markers such as CD34 high CD45 À CD31 À CD146 À adipose-derived stromal/stem cells (ADSCs), CD34 low CD45 + CD206 + CD31 À CD146 À hematopoietic stem cell-progenitors (HSC-progenitors), CD34 high CD45 À CD31 + CD146 + adipose tissue-endothelial cells and CD45 À CD34 À CD31 À CD146 + pericytes [24].…”