Effects of adding resorbable chitosan microspheres to calcium phosphate cements for bone regeneration

Meng, Dan; Dong, Limin; Wen, Ying; Xie, Qing

doi:10.1016/j.msec.2014.11.049

Cited by 56 publications

(23 citation statements)

References 43 publications

(41 reference statements)

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“…Chitosan, an ideal biomaterial with excellent biocompatibility and antibacterial capability, has been widely applied in tissue engineering and bone repair. [15][16][17][18][19] Therefore, we chose chitosan as the organic phase to fabricate the inorganic/organic composite porous scaffold for bone regeneration. First, we prepared HAP and WH hollow microspheres by using creatine phosphate (CP) disodium salt as an organic phosphorus source in aqueous solution by microwave-assisted hydrothermal method.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Zhou

Chen

et al. 2017

IJN

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Hydroxyapatite (HAP; Ca 10 (PO 4 ) 6 (OH) 2 ) and whitlockite (WH; Ca 18 Mg 2 (HPO 4 ) 2 (PO 4 ) 12 ) are widely utilized in bone repair because they are the main components of hard tissues such as bones and teeth. In this paper, we synthesized HAP and WH hollow microspheres by using creatine phosphate disodium salt as an organic phosphorus source in aqueous solution through microwave-assisted hydrothermal method. Then, we prepared HAP/chitosan and WH/chitosan composite membranes to evaluate their biocompatibility in vitro and prepared porous HAP/chitosan and WH/chitosan scaffolds by freeze drying to compare their effects on bone regeneration in calvarial defects in a rat model. The experimental results indicated that the WH/chitosan composite membrane had a better biocompatibility, enhancing proliferation and osteogenic differentiation ability of human mesenchymal stem cells than HAP/chitosan. Moreover, the porous WH/chitosan scaffold can significantly promote bone regeneration in calvarial defects, and thus it is more promising for applications in tissue engineering such as calvarial repair compared to porous HAP/chitosan scaffold.

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Section: Introductionmentioning

confidence: 99%

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Zhou

Chen

et al. 2017

IJN

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“…The polymers can provide better mechanical performance and also better properties, such as injectability, setting time, cohesion, degradation rate and biological response [18]. Chitosan [21], alginate [22], silk fibroin [23], collagen [24] and gelatin [25,26] are some examples. The formation of CPC/polymer composite follows the natural bone, that consists of apatite and fibrous collagen [7,27,28].…”

Section: Introductionmentioning

confidence: 99%

Brushite cement containing gelatin: evaluation of mechanical strength and in vitro degradation

et al. 2019

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Calcium phosphate cements (CPCs) are potential materials for repairing bone defects, mainly due to their excellent biocompatibility and osteoconductivity. Nevertheless, their low mechanical properties limit their usage in clinical applications. The gelatin addition may improve the mechanical and biological properties of CPCs, but their solubility in water may increase the porosity of the cement during degradation. Thus, the aim of this work was to investigate the influence of gelatin on the setting time, compressive strength and degradation rate of a brushite cement. CPCs were prepared with the addition of 0, 5, 10 and 20 wt% of gelatin powder in the solid phase of the cement. The results indicated that the setting time increased with gelatin. Furthermore, cement with 20 wt% of gelatin had an initial compressive strength of 14.1±1.8 MPa while cement without gelatin had 4.5±1.2 MPa. The weight loss, morphology and compressive strength were evaluated after degradation in Ringer’s solution. According to the weight loss data, gelatin was eliminated of samples during degradation. It was concluded that the presence of gelatin improved CPCs mechanical properties; however, as degradation in Ringer’s solution evolved, cement compressive strength decreased due to gelatin dissolution and, consequently, an increase in sample porosity.

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“…Biological fixation of the ligament to bone tissue is essential for the success of ligament reconstruction . For the enhancement of biological fixation, application of bioactive materials—such as calcium phosphate, hydroxyapatite, and composites containing chitin or chitosan—has been suggested. Although calcium phosphates are effective in bone formation, their bioabsorbabilities are relatively low, and the possibility was pointed that they prevent the maturation of insertions .…”

Section: Introductionmentioning

confidence: 99%

“…For the enhancement of biological fixation, application of bioactive materials—such as calcium phosphate, hydroxyapatite, and composites containing chitin or chitosan—has been suggested. Although calcium phosphates are effective in bone formation, their bioabsorbabilities are relatively low, and the possibility was pointed that they prevent the maturation of insertions . In contrast, chitin and chitosan are effective in promoting both bone tissues and soft connective tissues and exhibit relatively rapid absorption .…”

Section: Introductionmentioning

confidence: 99%

“…Although calcium phosphates are effective in bone formation, their bioabsorbabilities are relatively low, and the possibility was pointed that they prevent the maturation of insertions. 6 In contrast, chitin and chitosan are effective in promoting both bone tissues and soft connective tissues and exhibit relatively rapid absorption. [7][8][9][10] Hence, we previously utilized chitin and chitosan to achieve the biological fixation of artificial ligaments made of polyester fabrics to the bone and confirmed that they enhanced bone and soft connective tissue formation accompanying with increased attachment strength.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced biological fixation of ligaments to bone tissues utilizing chitin fabrics

2017

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In ligament reconstruction involving anterior cruciate ligament surgery, biological fixation between the transferred ligament and bone tissue is critical for achieving successful outcomes. Here, we administered chitin fabrics into the bone tunnels and evaluated their efficacy in promoting biological fixation. An animal model on the rat's patellar ligament was employed. First, bone tunnels were created in the lateral condyle of the femur. The ligament was then separated from the tibial tuberosity, and half was inserted into the tunnel and fixed with the use of end button. Animals in the experimental group were treated with microfiber nonwoven chitin fabric, whereas control animals received no treatment. Specimens were collected at 2, 4, and 6 weeks after surgery, and the fixation strength was measured by mechanical tests. Histological sections were prepared from samples prepared 4 weeks after surgery, and the diameter of bone tunnel and the width ratio of collagenous tissue in the bone tunnel were measured. Administration of chitin significantly increased the mean fixation strength at 4 and 6 weeks after surgery. Furthermore, chitin also promoted bone formation in the bone tunnel and increased the density of collagen fibers. Thus, microfiber nonwoven chitin fabric enhanced the biological fixation of the ligament to the bone tissue. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2355-2360, 2018.

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Effects of adding resorbable chitosan microspheres to calcium phosphate cements for bone regeneration

Cited by 56 publications

References 43 publications

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Comparative study of porous hydroxyapatite/chitosan and whitlockite/chitosan scaffolds for bone regeneration in calvarial defects

Brushite cement containing gelatin: evaluation of mechanical strength and in vitro degradation

Enhanced biological fixation of ligaments to bone tissues utilizing chitin fabrics

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