Effects of adding liquiddl‐methionine hydroxy analogue‐free acid to drinking water on growth performance and small intestinal morphology of nursery pigs

Abstract

This study was conducted to evaluate the effect of adding liquid DL-methionine hydroxy analogue free acid (LMA) to drinking water on growth performance, small intestinal morphology and volatile fatty acids in the caecum of nursery pigs. Twenty-four crossbred pigs (Large White x Landrace, BW approximately 18 kg) were divided into three groups with four replications of two piglets each. The piglets received drinking water without (control), with 0.05 or 0.10% LMA. The results indicated that adding LMA at 0.10% to drinking water significantly increased their weight gain, average daily feed intake (p < 0.05) and tended to improve the feed conversion ratio. Adding LMA to drinking water significantly increased their water intake and significantly reduced the pH of drinking water (p < 0.01), thus total plate count (p < 0.01) and Escherichia coli in drinking water was reduced (p < 0.05), while the total number of bacteria in the caecum was not significantly affected. Liquid DL-methionine hydroxy analogue free acid supplementation in drinking water tended to decrease pH in the stomach, duodenum, jejunum, colon and rectum. Furthermore, adding LMA at 0.10% significantly increased villous height in the duodenum, jejunum and ileum (p < 0.05), and the villous height:crypt depth ratio in the jejunum and ileum (p < 0.01) was higher, whereas acetic acid concentration in the caecum was significantly lower than in the control group. It could be concluded that adding LMA to drinking water improved growth performance of the nursery pigs because of high water quality and high nutrient utilization caused by an improvement of small intestinal morphology (not from nutritional effect of methionine source).

Evaluation of various sulphur amino acid to lysine ratio for growing-finishing pigs fed antibiotic-free diets

“…As expected, our study found that the villus-to-crypt ratio of small intestine were linearly improved by increasing dietary SID SAA:Lys. These findings are in agreement with the previous studies (Bauchart-Thevret et al 2009;Kaewtapee et al 2010;Kahindi 2014), they suggested that the improvement of small intestinal morphology as a result of increasing SAA or Met content in pigs diets. Therefore, it can be concluded that dietary SAA content promoted intestinal growth and abundant SAA could enhance enterocytes production by the crypt leading to longer villi.…”

Effects of different rearing systems on growth, small intestinal morphology and selected indices of fermentation status in broilers

“…The measurements of villus height and crypt depth were performed on stained sections under the microscope with an ocular micrometer and 40× combined magnification. Villus height was measured from the villus tip to the villus‐crypt junction, whereas crypt depth was measured from this junction to the base of the crypt (Kaewtapee et al ; Walsh et al ; Shen et al ). At least 10 well‐oriented and intact villi and their associated crypts were measured in each slide.…”

“…The microbial enumerations of cecum aerobes, Escherichia coli , Lactobacillus and Bifidobacteria , were determined using plate culture (Kaewtapee et al ). Cecal digesta were serially diluted with sterile physiological saline according to the procedure described by Engberg et al ().…”

“…VFA concentration was determined according to the method of Kaewtapee et al (). The ileum digesta samples were centrifuged at 17 800 × g and 4°C for 10 min, and 1.5 mL of supernatant was transferred to microfuge tubes.…”

Effect of source of methionine in broken rice-soybean diet on production performance, blood chemistry, and fermentation characteristics in weaned pigs

“…Due to the property of organic acids, Kaewtapee et al (2010) reported that adding LMA in drinking water reduced the total plate count and Escherichia coli (E. coli) in the water and consequently improved growth performance of piglets. In poultry, Poosuwan et al (2007) demonstrated that the minimum inhibitory concentration (MIC) of LMA for E. coli was 0.24% v/v in water, and this level promoted growth performance and tended to reduce E. coli in the gastrointestinal tract of broiler chicks.…”