Effect of source of methionine in broken rice-soybean diet on production performance, blood chemistry, and fermentation characteristics in weaned pigs

Abstract

ABSTRACT:This study was conducted to compare the effect of source of methionine (Met) in broken ricesoybean diet on performance, blood biochemistry, and fermentation characteristics in weaned pigs. Forty-eight male crossbreed pigs (BW 11 ± 0.1 kg) were randomly allocated to three groups with four replications in a completely randomized design. The experimental diets were: (1) basal diet without methionine (Control; total sulfur amino acids (TSAA) 0.60%); (2) basal diet supplemented with dl-methionine (DLM) (TSAA 0.76%); and (3) basal diet supplemented with dl-2-hydroxy-4-(methylthio) butanoic acid (LMA) (TSAA 0.76%). Supplementation with DLM and LMA improved growth performance of piglets and decreased blood urea nitrogen and increased serum albumin (P < 0.01). The population of Lactobacillus spp. in the caecum was decreased by both DLM and LMA supplementation (P < 0.05). Succinic acid concentration in the caecum of pigs fed the DLM diet was greater than that of LMA group (P < 0.05). It can be concluded that LMA can be used as a good source of Met (88% bioefficacy, weight/weight) in broken rice-soybean diet, although the serum albumin and fermentation characteristics (succinic acid) in the gastrointestinal tract were different.

Effects of Supplemental Liquid DL-methionine Hydroxy Analog Free Acid in Diet on Growth Performance and Gastrointestinal Functions of Piglets

“…The lower feed intake may explain the decrease in growth promotion of 0.24% group. Although the relative bioefficacy of DL-MHA to DL-Met has been reported to be lower than 88% ( Kim et al, 2006 ; Dilger and Baker, 2008 ), Bunchasak et al (2014) and Krutthai et al (2015) showed that 88% bioefficacy may be an acceptable with regard to SAA requirement. Therefore, based on 88% bioefficacy (w/w) compared to DL-Met, supplementation of DL-MHA at 0.15% and 0.24% of diet result in SID SAA:Lys ratio at 61% and 69%, respectively.…”

“…(1992) who reported that supplementation of organic acids in diet did not change the pH in the gut. Recently, Krutthai et al (2015) also reported low potentiality of acidifying by DL-MHA throughout gastrointestinal tract of piglets. Since supplemental DL-MHA induces dietary acidity ( Krutthai et al, 2015 ), the presence of an acidic diet may affect a low pH in stomach, leading to a negative feedback mechanism that inhibits HCl secretion ( Yen, 2001 ).…”

“…Methionine (Met) is considered as the second or third limiting amino acid in diet for modern nursery pigs ( Gaines et al, 2005 ), and it plays several roles such as an initiating amino acid in protein synthesis and as the principal biological methylating agent in the body ( Bunchasak, 2014 ). Commercially, both in powder DL-methionine (DL-Met, 99% powder) and its analogue liquid DL-methionine hydroxy analog free acid (DL-MHA, 88% aqueous solution) are supplemented in diets or drinking water for improving growth responses ( Kaewtapee et al, 2010 ; Krutthai et al, 2015 ). Although the composition or material used in diets such as practical, semi-purified, or purified diets influence the bioefficacy of DL-MHA compared to DL-Met ( Bunchasak, 2014 ), a smaller effect of DL-MHA and DL-Met can be seen when a practical diet formulated based on corn-soybean or broken rice-soybean is used ( Bunchasak, 2014 ; Krutthai et al, 2015 ).…”

“…Commercially, both in powder DL-methionine (DL-Met, 99% powder) and its analogue liquid DL-methionine hydroxy analog free acid (DL-MHA, 88% aqueous solution) are supplemented in diets or drinking water for improving growth responses ( Kaewtapee et al, 2010 ; Krutthai et al, 2015 ). Although the composition or material used in diets such as practical, semi-purified, or purified diets influence the bioefficacy of DL-MHA compared to DL-Met ( Bunchasak, 2014 ), a smaller effect of DL-MHA and DL-Met can be seen when a practical diet formulated based on corn-soybean or broken rice-soybean is used ( Bunchasak, 2014 ; Krutthai et al, 2015 ). In addition, in a broken rice-soybean based diet, Kongkaew (2014) concluded that bioefficacy of DL-MHA seemed to be 88% of DL-Met (w/w), and the standardized ileal digestible (SID) of sulfur amino acids (SAA) to Lys (SID SAA:Lys) ratio for maximum growth rate was around 0.60 to 0.63.…”

Effect of capsaicin and capsaicin plus DL-methionine hydroxy analog in diet on growth performance and gastrointestinal conditions of nursery pigs

“…The samples of caecum digesta were centrifuged for 10 min at 14,000xg at 4ºC and the supernatants were sampled to determine the lactic acid and short-chain fatty acids (SCFAs) concentrations. The concentrations of lactic acid and SCFAs were determined under the following conditions using a HPLC (Krutthai et al 2015).…”