Effects of Acute Water Load, Hypertonic Saline Infusion, and Furosemide Administration on Atrial Natriuretic Peptide and Vasopressin Release in Humans*

Abstract

A new specific RIA for alpha-human atrial natriuretic hormone (alpha hANP) was used to determine whether changes in plasma volume elicited by acute water loading, hypertonic saline infusion, and furosemide administration caused changes in ANP release and resultant changes in renal and cardiovascular function in normal subjects. In addition, changes in plasma arginine vasopressin (AVP), PRA, and aldosterone concentrations were studied simultaneously. Mean plasma alpha hANP and AVP levels were 51.3 +/- 16.0 (+/- SE) and 3.1 +/- 0.6 pg/ml, respectively, in the basal state. Plasma alpha hANP rose to 77.8 +/- 27.6 in response to a 4.5% increase in plasma volume induced by water loading, increased further to 134.1 +/- 28.9 in response to a 23% volume increase induced by hypertonic saline, and fell to 70.2 +/- 15.8 pg/ml in response to a decrease in plasma volume after furosemide treatment (P less than 0.01-0.05). On the other hand, plasma AVP fell to 1.8 +/- 0.1 pg/ml after the water load, rose to 4.1 +/- 0.6 after hypertonic saline, and rose further to 5.8 +/- 0.8 pg/ml after furosemide (P less than 0.01-0.05). Water and hypertonic saline loading decreased PRA, but plasma aldosterone concentrations did not change; subsequent furosemide administration increased both (P less than 0.01-0.05). Arterial pressure and heart rate did not change significantly. Increases in urinary Na excretion and osmolar clearances were associated with a rise in plasma alpha hANP after water loading and hypertonic saline infusion (P less than 0.01-0.05), but changes in urine flow were mainly associated with alterations in AVP release. associated with alterations in AVP release.

Hormonal replacement therapy affects calcitonin gene-related peptide and atrial natriuretic peptide secretion in postmenopausal women

“…Circulating levels of ANP are elevated when intravascular volume or right atrial pressure is increased (12). Saline infusion has been shown to increase circulating ANP levels in healthy subjects (13). Infusion of clonidine, an a 2 -adrenergic agonist, has also been shown to produce a marked increase in plasma ANP levels in healthy subjects (14).…”

Role of Atrial Natriuretic Peptide in Interleukin 1.BETA.-Induced Natriuresis in Conscious Rats.

“…), respectively. Inter-and intra-assay coefficients of variation in the AVP and ANP RIAs were 9.4 and 3.7% and 14.2 and 9.5%, respectively (Kimura et al 1986). …”

“…Measurements of A VP and ANP The radioimmunoassay (RIA) for AVP and ANP were previously reported (Kimura et al 1986). Briefly, AVP and ANP were extracted using octadecasylsilane packed in a cartridge (Sep-Pak C18 cartridge, Waters Associates, Milford, MA, USA) and were assayed using specific antibodies (Mitsubishi Petrochemical, Tokyo) to AVP and ANP.…”

Effects of antihypertensive drugs on renal function and atrial natriuretic polypeptide in spontaneously hypertensive rats with renal ablation.

“…Plasma samples were immediately mixed with 0.1 N HCl and kept at -20°C. The concentration of ANP was measured by the modified method reported previously (Kimura et al 1986). Radioimmunoassay was performed after extraction of acidified plasma through C18 sep-pak cartridges with 80% acetone-HC1 (pH 1.5).…”