Effects of acute and chronic protein deprivation on ornithine decarboxylase levels in rat liver and colon

Abstract

The effects of protein intake on the levels of total and holo-ornithine decarboxylase (ODC) in colon and liver have been examined in male Sprague-Dawley rats fed varying amounts of casein in a defined purified diet. Pair-feeding was used to eliminate effects of food intake per se. In the colon, both total and holo-ODC activity increased in animals fed a low-protein diet (1% casein). In the liver, on the other hand, both total and holo-ODC activity decreased with protein deprivation. The observed changes in both colon and liver appeared to be maximal within several days after institution of the dietary regimen. Refeeding with a high-protein diet (20% casein) led to a dramatic rise in ODC activity in the liver in animals previously fed a low-protein diet. These results may be important in sorting out the effects of diet on the tumorigenic process.

Characterization of ion interference in gossypol consumption

“…Lactate dehydrogenase was determined by the methods of Siegler and Kazarinoff [5]. Glutathione level was determined by the method of Reed [6].…”

Analysis of Pesticide Raid® in Feed of Wistar Rat by High-Pressure Liquid Chromatography (HPLC)

“…The brain, muscle and liver homogenate fractions were prepared by the method described by [12] and resuspended in 0.15 M KCl to an appropriate final concentration of 20 mg of protein per millimeter (approximately) 0.5 g of tissue. Liver tissue lipids were extracted by chloroform/methanol ratio (2:1) following the method of Folch [13].…”