Effects of acute and chronic prednisolone treatment on serum zinc levels in rats with adjuvant arthritis

Fontaine, Jeanine; Nève, Jean; Peretz, Anne; Capel, Paul D.; Famaey, Jean Pierre

doi:10.1007/bf01986570

Cited by 12 publications

(5 citation statements)

References 28 publications

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“…Yet this latter group showed significant additional improvement in the α -tocopherol/ γ -tocopherol ratio and plasma selenium, indicating that supplementation was efficient [26]. Zinc was also improved in the supplemented group, although the tendency for additional improvement versus placebo ( p = 0.096) was not significant after adjustment on corticosteroid use ( p = 0.390), which increases the plasma zinc level [24] and may thus have masked the effect of zinc supplementation in our study.…”

Section: Discussionmentioning

confidence: 99%

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Additional Effects of Nutritional Antioxidant Supplementation on Peripheral Muscle during Pulmonary Rehabilitation in COPD Patients: A Randomized Controlled Trial

Gouzi

Maury

Héraud³

et al. 2019

Oxidative Medicine and Cellular Longevity

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Background. Skeletal muscle dysfunction in patients with chronic obstructive pulmonary disease (COPD) is not fully reversed by exercise training. Antioxidants are critical for muscle homeostasis and adaptation to training. However, COPD patients experience antioxidant deficits that worsen after training and might impact their muscle response to training. Nutritional antioxidant supplementation in combination with pulmonary rehabilitation (PR) would further improve muscle function, oxidative stress, and PR outcomes in COPD patients. Methods. Sixty-four COPD patients admitted to inpatient PR were randomized to receive 28 days of oral antioxidant supplementation targeting the previously observed deficits (PR antioxidant group; α-tocopherol: 30 mg/day, ascorbate: 180 mg/day, zinc gluconate: 15 mg/day, selenomethionine: 50 μg/day) or placebo (PR placebo group). PR consisted of 24 sessions of moderate-intensity exercise training. Changes in muscle endurance (primary outcome), oxidative stress, and PR outcomes were assessed. Results. Eighty-one percent of the patients (FEV1=58.9±20.0%pred) showed at least one nutritional antioxidant deficit. Training improved muscle endurance in the PR placebo group (+37.4±45.1%, p<0.001), without additional increase in the PR antioxidant group (-6.6±11.3%; p=0.56). Nevertheless, supplementation increased the α-tocopherol/γ-tocopherol ratio and selenium (+58±20%, p<0.001, and +16±5%, p<0.01, respectively), muscle strength (+11±3%, p<0.001), and serum total proteins (+7±2%, p<0.001), and it tended to increase the type I fiber proportion (+32±17%, p=0.07). The prevalence of muscle weakness decreased in the PR antioxidant group only, from 30.0 to 10.7% (p<0.05). Conclusions. While the primary outcome was not significantly improved, COPD patients demonstrate significant improvements of secondary outcomes (muscle strength and other training-refractory outcomes), suggesting a potential “add-on” effect of the nutritional antioxidant supplementation (vitamins C and E, zinc, and selenium) during PR. This trial is registered with NCT01942889.

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Section: Discussionmentioning

confidence: 99%

“…Samples were immediately centrifuged and frozen (-80°C). Determination of CRP was run on the Cobas 8000/e502® analyzer (Roche Diagnostics, Meylan, France) using the immunoturbidimetric method [24].…”

Section: Methodsmentioning

confidence: 99%

Additional Effects of Nutritional Antioxidant Supplementation on Peripheral Muscle during Pulmonary Rehabilitation in COPD Patients: A Randomized Controlled Trial

Gouzi

Maury

Héraud³

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Zinc level alterations have been postulated to reflect cumulative underlying immunological processes that cannot be mirrored by a disability score taken at a single time point. In particular, the absence of an acute clinical deterioration could explain the generally lower, though still within the normal range, zinc levels, which can be observed in acute clinical relapses or due to acute prednisolone therapy [ 23 , 31 ]. Despite the absence of a relationship between zinc levels and depression prevalence, recent preliminary results suggest that zinc supplementation in MS can improve psychiatric symptoms, which could be due to a zinc deficiency in both disease entities [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…We consider here the possible reasons for the lack of zinc deficiency as well as differences between the zinc ranges observed in the current study and those reported elsewhere. The normal zinc range might be explained by the low rate of zinc-reducing confounding factors such as diabetes [ 12 ] or vegetarian diet [ 13 ], as well as the exclusion of pregnant patients [ 33 ] and those receiving prednisone treatment [ 31 ]. Had these factors been present in our cohort and equally distributed across the MS patients and HCs, we might expect that the inter-group difference we detected would still have been present, but that the zinc levels in the MS patient group would have fallen below the normal range.…”

Section: Discussionmentioning

confidence: 99%

Lower Serum Zinc Levels in Patients with Multiple Sclerosis Compared to Healthy Controls

et al. 2018

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Objective: Diminished blood levels of zinc have been reported to be associated with T-cell-mediated autoimmunity, which has been implicated in multiple sclerosis (MS). We aimed to compare the distribution of serum zinc status in MS patients with that in healthy controls (HCs) and to investigate a potential correlation with clinical state, through analysis of serum zinc concentration in MS patients suffering from different disease subtypes. Methods: Serum zinc concentrations of 133 patients with relapsing (RMS) and 18 patients with the progressive form of MS (PMS), according to the McDonald criteria of 2010, were measured. Clinical status was quantified using the Expanded Disability Status Scale (EDSS). Zinc concentrations were also determined in the sera of 50 HCs, matched for age and sex at a group level. Results: MS patients showed significantly lower zinc concentrations (mean (SD)) than HCs (12.5 (2.1) µmol/L vs. 14.6 (2.3) µmol/L, p < 0.001). In contrast, we did not find any difference between RMS (12.4 (2.0) µmol/L) and PMS (13.0 (3.0) µmol/L) cases (p = 0.8). Patients receiving disease-modifying treatment showed lower mean (SD) serum zinc levels than untreated cases (12.3 (1.9) µmol/L vs. 13.5 (3.2) µmol/L, p < 0.03). Zinc levels were not related to disease duration, EDSS, annual relapse rate, or the median number of relapses. Conclusions: The data suggest that a diagnosis of MS is related to lower serum zinc concentrations than in HCs, and concentrations were lower still under disease-modifying therapy. However, zinc levels did not predict disease subtypes or disability status.

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“…The involvement of catabolic hormones (glucocorticoids and catecholamine) in weight loss and altered zinc homeostasis in arthritis must also be considered. [ 14 ] The present study clarified that the normal control group showed an increase in the body weight of rats while rats of the CFA group showed no weight gain till the 10 th day suggesting catabolic effects during arthritic conditions. Rhuflex-F at both dose levels depicted a significant increase in body weight compared to initial body weight recommending, anabolic effects of Rhuflex-F against Freund’s adjuvant-induced arthritis in albino rats.…”

Section: Resultsmentioning

confidence: 51%

Evaluation of the anti-arthritic activity of Rhuflex-F – A proprietary Ayurvedic herbomineral formulation in albino rats

Gupta,

Panigrahi,

et al. 2023

AYU (An International Quarterly Journal of Research in Ayurveda)

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Background: Rhuflex-F is a proprietary Ayurvedic herbo-mineral formulation clinically used to combat and relieve stiffness in joints and muscles, reduce edema, restore mobility, and also effective in relieving the symptoms of other autoimmune illnesses that lead to rheumatism. Aims: The aim and objective of the research study is to evaluate the efficacy of Rhuflex-F against in vitro protein denaturation and in vivo Freund’s adjuvant-induced arthritis in albino rats. Materials and methods: In vitro inhibition of protein denaturation activity was carried out using bovine serum albumin. For in vivo activity, arthritis was induced by complete Freund’s adjuvant in albino rats. Rhuflex-F (135–270 mg/kg, po) was administered for 30th days in arthritic rats, and effects were assessed on primary and secondary paw edema, on pain response, hematological, serum biochemical parameters (serum transaminases, alkaline phosphatase, urea, uric acid, and orosomucoid), and serum anti-oxidant parameters and adrenal ascorbic acid. Results: Aqueous extract of Rhuflex-F showed in vitro protein denaturation inhibitory activity in a dose-dependent manner. Rhuflex-F showed nonsignificant decrease in primary and secondary paw edema with reduced pain response, some reversal effects on hematological parameters such as white blood cell and red blood cell related parameters and serum orosomucoid and adrenal ascorbic acid in comparison to Fruend’s adjuvant control group. Further, Rhuflex-F reversed Freund’s adjuvant-induced adverse effects on oxidant status in the serum of albino rats. Conclusion: Result of the present study suggested that Rhuflex-F formulation has anti-inflammatory activity, may be due to the inhibition of protein denaturation in vitro and in vivo anti-arthritic activity against complete Freund’s adjuvant-induced arthritis in albino rats.

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Effects of acute and chronic prednisolone treatment on serum zinc levels in rats with adjuvant arthritis

Cited by 12 publications

References 28 publications

Additional Effects of Nutritional Antioxidant Supplementation on Peripheral Muscle during Pulmonary Rehabilitation in COPD Patients: A Randomized Controlled Trial

Additional Effects of Nutritional Antioxidant Supplementation on Peripheral Muscle during Pulmonary Rehabilitation in COPD Patients: A Randomized Controlled Trial

Lower Serum Zinc Levels in Patients with Multiple Sclerosis Compared to Healthy Controls

Evaluation of the anti-arthritic activity of Rhuflex-F – A proprietary Ayurvedic herbomineral formulation in albino rats

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