Effects of acetylsalicylic acid on serum paraoxonase activity, Ox-LDL, coenzyme Q10 and other oxidative stress markers in healthy volunteers

Kurban, Sevil; Mehmetoğlu, İdris

doi:10.1016/j.clinbiochem.2009.10.054

Cited by 24 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This increase was marked especially after 24h and one week of incubation. These results confirm those of Kurban and Mehmetoglu [3], which showed that the activity of PON was higher in volunteers treated with salicylates for one week. Recent studies [5] reported that aspirin users have increased PON activity in the plasma and results of Cyrus et al [6] and Maree and Fitzgerald [7] suggest that aspirin is an antiatherogenic drug.…”

Section: Discussionsupporting

confidence: 82%

“…But, aspirin's effects on platelets alone cannot explain its ability to inhibit atherosclerosis. Indeed, there is very little evidence to indicate that involves aggregated platelets until the terminal consequences of plaque rupture [3].…”

Section: Discussionmentioning

confidence: 99%

“…Human serum PON1 activity was reported to be inversely related to the risk of coronary artery disease (CAD) [3]. Its activity decreases in cardiovascular disease and in atherosclerotic, hypercholesterolemic, hypertensive and diabetic patients [3]. Our study aimed to investigate the in vitro effects of salicylic acid on human plasma paraoxonase 1 activity.…”

Section: Introductionmentioning

confidence: 99%

“…Paraoxonase 1, a high-density lipoprotein associated antioxidant enzyme, has been shown to protect the serum lipids from oxidation and can reduce macrophage foam cell formation and attenuates atherosclerosis development. Human serum PON1 activity was reported to be inversely related to the risk of coronary artery disease (CAD) [3]. Its activity decreases in cardiovascular disease and in atherosclerotic, hypercholesterolemic, hypertensive and diabetic patients [3].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

Mouhamed¹

2013

J Drug Metab Toxicol

View full text Add to dashboard Cite

Objectives: Our study aimed to examine the effects of salicylic acid on paraoxonase activity in healthy volunteers. Methods: Plasma from healthy volunteers was spiked with salicylic acid drug. The working solutions were then diluted with plasma to obtain concentrations that covered the therapeutic margin. Specimens were incubated at 25 and 37°C, and we measured PON1 activities in the presence of different drug concentrations after 1 h, 3 h, and 24 h and after one week.Results: After incubation in vitro, a significant increase of paraoxonase 1 activity according to salicylic acid concentration and incubation duration, regardless of temperature incubation was showed. This increase was marked especially after 24 h of incubation. The stimulatory effect of salicylic acid is concentration and incubation durationdependent and does not seem to be related to temperature incubation. Conclusion:The tested Salicylic acid significantly stimulated PON1 activity in a concentration dependent manner. This result shows that acetyl salicylic acid treatments have a beneficial effect dose and time dependent that may contribute to prevent atherosclerosis.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

Mouhamed¹

2013

J Drug Metab Toxicol

View full text Add to dashboard Cite

show abstract

“…Costa et al 25 reported that modulation of PON1 have involved pharmaceutical drugs, particularly lipid-lowering compounds such as statins and fibrates, as well as some other drugs [26][27][28][29][30] . A few studies have investigated the effects of some lipid-lowering agents on serum paraoxonase activity [31][32][33][34] .…”

Section: Discussionmentioning

confidence: 99%

Antidepressant and antipsychotic drugs differentially affect PON1 enzyme activity

Avcıkurt

Sinan

Köçkar

2014

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

Human serum paraoxonase (PON1, EC 3.1.8.1.) is a high-density lipid (HDL)-associated, calciumdependent enzyme. In this study, the effects of Haloperidol, Fluoxetine hydrochloride, Diazepam and Acepromazine drugs used for the therapy of antidepressant and antipsychotic diseases, on paraoxonase enzyme activity was studied in in vitro inhibition studies on purified human serum PON1. PON1 enzyme was purified from human blood using two-step procedures, namely, ammonium sulfate precipitation and sepharose-4B-l-tyrosine-1-napthylamine hydrophobic interaction chromatography. The overall purification of human serum PON1 was obtained in a activity of 109.29 U/mL and this enzyme was purified 125-fold. The SDSpolyacrylamide gel electrophoresis of the enzyme indicates a single band with an apparent MW of 43 kDa. Inhibition studies indicated that haloperidol and fluoxetine hydrocloride were effective inhibitors on purified human serum PON1 activity with IC 50 of 0.187 and 3.08 mM values, respectively. The kinetics of interaction of haloperidol and fluoxetine hydrocloride with the purified human serum PON1 indicated uncompetitive inhibiton pattern with K i of 4.15 and 0.007 mM, respectively.

show abstract

Paraoxonases: metabolic role and pharmacological projection

Moya

Máñez

2018

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Atherosclerosis is one of the leading causes of death in Western countries, with high-density lipoproteins (HDL) playing an important protective role due to their ability to inhibit oxidation of low-density lipoproteins (LDL), thus relieving vascular subendothelial damage. One of the proteins constituting HDL particles is paraoxonase-1 (PON1), an enzyme able to hydrolyze aryl esters, lactones, and organophosphates. Other closely related paraoxonases are designated as PON2, which is a protein localized inside many different kinds of cells, and PON3, not only present in HDL but also in mitochondria and endoplasmic reticulum, as well. Given that the amount and the activity of PON1 in human serum are significantly lower in people suffering from cardiovascular diseases, enhancing both parameters might contribute to their treatment and prevention. One of the physiologically interesting substrates for the abovementioned hydrolytic cleavage is homocysteine thiolactone (HTL), an atherothrombotic active form of homocysteine. Although it was therefore postulated that PON1 would participate in preventing the HTL-mediated lipid peroxidation, some attention is recently paid to other enzymes, like biphenyl hydrolase-like protein, that seem to more selectively involved in lowering this risk factor. The aim of this paper is to elucidate the role of paraoxonases, especially PON1, by reviewing the latest studies in order to understand both its physiological role and modulation by drugs, nutrients, and plant extracts.

show abstract

Effects of acetylsalicylic acid on serum paraoxonase activity, Ox-LDL, coenzyme Q10 and other oxidative stress markers in healthy volunteers

Cited by 24 publications

References 30 publications

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

In vitro Effects of Salicylic Acid on Plasma Paraoxonase 1 Activity

Antidepressant and antipsychotic drugs differentially affect PON1 enzyme activity

Paraoxonases: metabolic role and pharmacological projection

Contact Info

Product

Resources

About