Effects of acclimation temperature on thermal tolerance and membrane phospholipid composition in the fruit fly Drosophila melanogaster

Overgaard, Johannes; Tomčala, Aleš; Sørensen, Jesper Givskov; Holmstrup, Martin; Krogh, Paul Henning; Šimek, Petr; Košťál, Vladimı́r

doi:10.1016/j.jinsphys.2007.12.011

Cited by 143 publications

(164 citation statements)

References 38 publications

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“…Generally, these adjustments increase membrane fluidity, through, for instance, an increase in the degree of membrane unsaturation (Bennett et al, 1997), an increase in PE/PC ratio (Overgaard et al, 2008) or an overall shortening of FAs (Michaud and Denlinger, 2006). However, in the current study no clear signs of cold tolerance or low-temperature functionality-related acclimatory changes were seen, except for an increase in PE/PC ratio when comparing 6 day old diapausing pupae with 24 and 144 day old pupae (from 1.5± 0.04 to 1.9±0.07).…”

Section: Lipidome Changes During Diapause and Developmentmentioning

confidence: 99%

Energy and lipid metabolism during direct and diapause development in a pierid butterfly

Lehmann

Pruisscher

Posledovich

et al. 2016

Journal of Experimental Biology

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Diapause is a fundamental component of the life cycle in the majority of insects living in environments characterized by strong seasonality. The present study addresses poorly understood associations and trade-offs between endogenous diapause duration, thermal sensitivity of development, energetic cost of development and cold tolerance. Diapause intensity, metabolic rate trajectories and lipid profiles of directly developing and diapausing animals were studied using pupae and adults of Pieris napi butterflies from a population in which endogenous diapause has been well studied. Endogenous diapause was terminated after 3 months and termination required chilling. Metabolic and post-diapause development rates increased with diapause duration, while the metabolic cost of post-diapause development decreased, indicating that once diapause is terminated, development proceeds at a low rate even at low temperature. Diapausing pupae had larger lipid stores than the directly developing pupae, and lipids constituted the primary energy source during diapause. However, during diapause, lipid stores did not decrease. Thus, despite lipid catabolism meeting the low energy costs of the diapausing pupae, primary lipid store utilization did not occur until the onset of growth and metamorphosis in spring. In line with this finding, diapausing pupae contained low amounts of mitochondria-derived cardiolipins, which suggests a low capacity for fatty acid β-oxidation. While ontogenic development had a large effect on lipid and fatty acid profiles, only small changes in these were seen during diapause. The data therefore indicate that the diapause lipidomic phenotype is developed early, when pupae are still at high temperature, and retained until post-diapause development.

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Section: Lipidome Changes During Diapause and Developmentmentioning

confidence: 99%

Energy and lipid metabolism during direct and diapause development in a pierid butterfly

Lehmann

Pruisscher

Posledovich

et al. 2016

Journal of Experimental Biology

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“…The physiological changes leading to increased cold tolerance during cold acclimation have been well studied in insects and are known to primarily involve a shift in the metabolic profile of the insect, particularly sugars, polyols and amino acids (Lee, 1991;Lee, 2010). Accompanying this is a compositional change of the cell membrane primarily involving changes in phospholipids (Hazel, 1995;Koštál et al, 2003;Overgaard et al, 2008). These changes are cryoprotective, allowing cells to maintain their osmotic balance and thus to continue to function at low temperatures.…”

Section: Introductionmentioning

confidence: 99%

How consistent are the transcriptome changes associated with cold acclimation in two species of the Drosophila virilis group?

et al. 2015

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For many organisms the ability to cold acclimate with the onset of seasonal cold has major implications for their fitness. In insects, where this ability is widespread, the physiological changes associated with increased cold tolerance have been well studied. Despite this, little work has been done to trace changes in gene expression during cold acclimation that lead to an increase in cold tolerance. We used an RNA-Seq approach to investigate this in two species of the Drosophila virilis group. We found that the majority of genes that are differentially expressed during cold acclimation differ between the two species. Despite this, the biological processes associated with the differentially expressed genes were broadly similar in the two species. These included: metabolism, cell membrane composition, and circadian rhythms, which are largely consistent with previous work on cold acclimation/cold tolerance. In addition, we also found evidence of the involvement of the rhodopsin pathway in cold acclimation, a pathway that has been recently linked to thermotaxis. Interestingly, we found no evidence of differential expression of stress genes implying that long-term cold acclimation and short-term stress response may have a different physiological basis.

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“…For instance, Berrigan (1997) showed that the flies developed at 29°C had a lower mass-specific metabolic rate than the flies with cold-acclimation at 18°C. Overgaard et al (2008) reported that the flies reared at 25°C had a lower cold tolerance compared to the control flies acclimatized at 20°C. Although these studies were designed to examine the effect of the rearing temperature rather than the acclimation, we clearly showed in this study that the effect of rearing temperature is mostly overridden by the temperature experienced at the adult stage using the deacclimation and reacclimation experiments.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in lipid composition of cell membrane have been identified as a molecular mechanism underlying the rearing acclimation and the rapid cold hardening in insects (Ohtsu et al, 1998;Lee et al, 2006;Overgaard et al, 2008), whereas changes in metabolic rate are thought to be a mechanism for the cold acclimation. For instance, Berrigan (1997) showed that D. melanogaster flies reared at 18°C had a higher respiration rate (metabolic rate) at 15°C compared to the flies reared at 25°C or 29°C.…”

Section: Introductionmentioning

confidence: 99%

Cold tolerance and metabolic rate increased by cold acclimation in <i>Drosophila albomicans</i> from natural populations

2013

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Cold acclimation is one of the important factors in temperature adaptation for insects needing to make rapid adjustment to the seasonal temperature changes in their living environment. In a fruit fly species, Drosophila albomicans, which has a tropical origin and currently has a wide geographic distribution extended into Asian temperate regions, cold tolerance in terms of survival time at 1°C of adult flies reared at 25°C was substantially improved by a cold acclimation at 20°C for several days. Examining 29 isofemale lines from widely distributed natural populations, we observed a substantial variation in their acclimation response. However, the acclimation response was not necessarily stronger in the strains from the recently colonized temperate regions. A significantly stronger acclimation response was detected in male flies of the temperate strains when compared to those of the tropical strains. D. albomicans also showed stronger cold tolerance compared to its closely related species belonging to the D. nasuta subgroup. Among these strains, we detected a strong positive correlation between the cold tolerance change and the metabolic rate change upon the cold acclimation, suggesting their strong physiological association regulated by common genetic factors, which may have been the target of natural selection for the temperature adaptation. The response to deacclimation and reacclimation suggested that a systematic change in gene expressions is the main molecular mechanism for the cold acclimation to have effects on the cold tolerance and metabolic rate changes.

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Effects of acclimation temperature on thermal tolerance and membrane phospholipid composition in the fruit fly Drosophila melanogaster

Cited by 143 publications

References 38 publications

Energy and lipid metabolism during direct and diapause development in a pierid butterfly

Energy and lipid metabolism during direct and diapause development in a pierid butterfly

How consistent are the transcriptome changes associated with cold acclimation in two species of the Drosophila virilis group?

Cold tolerance and metabolic rate increased by cold acclimation in <i>Drosophila albomicans</i> from natural populations

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