How consistent are the transcriptome changes associated with cold acclimation in two species of the Drosophila virilis group?

Parker, Darren J.; Vesala, Laura; Ritchie, Michael G.; Laiho, Asta; Hoikkala, Anneli; Kankare, Maaria

doi:10.1038/hdy.2015.6

Cited by 47 publications

(77 citation statements)

References 64 publications

(76 reference statements)

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“…Parker et al . [17] used an RNA-seq approach to identify genes which change expression during cold acclimation in D . montana , an extremely cold-adapted species.…”

Section: Discussionmentioning

confidence: 99%

“…Note the methodology decribed above is similar to that used by Parker et al . [17] to allow our results to be easily compared.…”

Section: Methodsmentioning

confidence: 99%

“…virilis and D . montana found that a number of differentially expressed genes were common to both species [17]. Although these species are relatively closely related, they have different cold tolerances as measured by chill coma recovery time [18].…”

Section: Introductionmentioning

confidence: 99%

“…[17] myo-inositol-1-phosphate synthase (Inos) stands out as a plausible candidate given what is known about its function. Inos encodes the enzyme myo-inositol-1-phosphate synthase which is the rate-limiting step in myo-inositol biosynthesis [19], the major metabolite produced during overwintering by D .…”

Section: Introductionmentioning

confidence: 99%

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Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi

et al. 2016

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Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By increasing their cold tolerance individuals can remain active for longer at the onset of winter and can recover more quickly from a cold shock. In insects, despite many physiological studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate synthase (Inos), in cold tolerance in D. montana using an RNAi approach. D. montana has a circumpolar distribution and overwinters as an adult in northern latitudes with extreme cold. We assessed cold tolerance of dsRNA knock-down flies using two metrics: chill-coma recovery time (CCRT) and mortality rate after cold acclimation. Injection of dsRNAInos did not alter CCRT, either overall or in interaction with the cold treatment, however it did induced cold-specific mortality, with high levels of mortality observed in injected flies acclimated at 5°C but not at 19°C. Overall, injection with dsRNAInos induced a temperature-sensitive mortality rate of over 60% in this normally cold-tolerant species. qPCR analysis confirmed that dsRNA injection successfully reduced gene expression of Inos. Thus, our results demonstrate the involvement of Inos in increasing cold tolerance in D. montana. The potential mechanisms involved by which Inos increases cold tolerance are also discussed.

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“…Parker et al . [17] used an RNA-seq approach to identify genes which change expression during cold acclimation in D . montana , an extremely cold-adapted species.…”

Section: Discussionmentioning

confidence: 99%

“…Note the methodology decribed above is similar to that used by Parker et al . [17] to allow our results to be easily compared.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi

et al. 2016

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“…The circum-arctic species Drosophila montana has proved to be a good species to study adaptive traits, including cold tolerance, developmental time, juvenile body weight and reproductive diapause (Vesala and Hoikkala, 2011;Salminen et al, 2012;Parker et al, 2015) in the wild. Phylogeographic analysis of the species based on mitochondrial DNA and microsatellites suggests two clades, a North-American and European, that were isolated about 0.5 Myr ago .…”

Section: Introductionmentioning

confidence: 99%

The genetic architecture of sexually selected traits in two natural populations of Drosophila montana

et al. 2015

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We investigated the genetic architecture of courtship song and cuticular hydrocarbon traits in two phygenetically distinct populations of Drosophila montana. To study natural variation in these two important traits, we analysed within-population crosses among individuals sampled from the wild. Hence, the genetic variation analysed should represent that available for natural and sexual selection to act upon. In contrast to previous between-population crosses in this species, no major quantitative trait loci (QTLs) were detected, perhaps because the between-population QTLs were due to fixed differences between the populations. Partitioning the trait variation to chromosomes suggested a broadly polygenic genetic architecture of within-population variation, although some chromosomes explained more variation in one population compared with the other. Studies of natural variation provide an important contrast to crosses between species or divergent lines, but our analysis highlights recent concerns that segregating variation within populations for important quantitative ecological traits may largely consist of small effect alleles, difficult to detect with studies of moderate power.

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Divergence and introgression among the virilis group of Drosophila

et al. 2022

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Speciation with gene flow is now widely regarded as common. However, the frequency of introgression between recently diverged species and the evolutionary consequences of gene flow are still poorly understood. The virilis group of Drosophila contains 12 species that are geographically widespread and show varying levels of prezygotic and postzygotic isolation. Here, we use de novo genome assemblies and whole-genome sequencing data to resolve phylogenetic relationships and describe patterns of introgression and divergence across the group. We suggest that the virilis group consists of three, rather than the traditional two, subgroups. Some genes undergoing rapid sequence divergence across the group were involved in chemical communication and desiccation tolerance, and may be related to the evolution of sexual isolation and adaptation. We found evidence of pervasive phylogenetic discordance caused by ancient introgression events between distant lineages within the group, and more recent gene flow between closely related species. When assessing patterns of genome-wide divergence in species pairs across the group, we found no consistent genomic evidence of a disproportionate role for the X chromosome as has been found in other systems. Our results show how ancient and recent introgressions confuse phylogenetic reconstruction, but may play an important role during early radiation of a group.

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How consistent are the transcriptome changes associated with cold acclimation in two species of the Drosophila virilis group?

Cited by 47 publications

References 64 publications

Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi

Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi

The genetic architecture of sexually selected traits in two natural populations of Drosophila montana

Divergence and introgression among the virilis group of Drosophila

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