Effects of Abdominal Surgery on Thiobarbituric acid Reactive Substances and Plasma Anti‐oxidative Capacity in Dairy Cows

Abstract

The present investigation was undertaken to study the effects of surgical stress on thiobarbituric acid reactive substances (TBARS) and ferric reducing ability of plasma (FRAP) in dairy cows. Ten Holstein-Friesian dairy cows, mean age 5.26 years, admitted for treatment of left abomasal displacement (omentopexy), were used in our study. Blood samples were collected from the jugular vein prior to surgery, immediately and then 15, 30, 60, 90 min, and 2, 5, 10, and 24 h after reposition of abomasum. Surgical stress resulted in a significant increase in plasma cortisol concentrations (P < 0.001) with the highest mean levels 15 min after surgery (32.4 +/- 18.1 microg/l) as well as in serum levels of glucose (P < 0.001) and non-esterified fatty acids (P < 0.001). Similarly, surgery transiently enhanced the plasma levels of TBARS (P < 0.001). The highest TBARS plasma levels (0.91 +/- 0.18 micromol/l) were recognized 60 min after reposition of abomasums. There were no significant differences in FRAP in dairy cows before and after reposition of abomasum. In conclusion, these data indicate that stress reaction caused by surgical correction of left displacement of abomasum can be associated with higher production of TBARS.

Dose-response effect of fish oil substitution in parturition feed on erythrocyte membrane characteristics and sow performance

“…This apparent contradiction between TBARS and FRAP can be explained by the hypothesis that with increased lipid peroxidation, antioxidant mechanisms of the sow are activated that resulted in higher FRAP levels when higher portions of fish oil were present in the diet. A lack of positive correlation between TBARS and FRAP measurements is also described in other studies (Hadi et al., 2004; Mudron et al., 2007).…”

Oxidative stress and antioxidant status in dairy cows during prepartal and postpartal periods

“…In the present study, the highest MDA production of dairy cows was determined in the first week after parturition. Similarly to our results, many authors have also recorded a tendency to transient increase of lipid peroxidation in dairy cows in the same period after calving (Mudron and Konvicna 2006;Saleh et al 2007;Sharma et al 2011). This change is typical for this period, and increased intake and higher levels of nutritive antioxidants can efficiently decrease the level of lipid peroxidation (Brzezinska-Slebodzinska et al 1993).…”

Effects of vitamin E and selenium supplementation on blood lipid peroxidation and cortisol concentration in dairy cows undergoing omentopexy

“…In addition, TBARS are considered inaccurate, as they detect a wide range of lipid peroxidation products and are not specific for MDA (Halliwell & Chirico, ). In our previous experiment, the abdominal surgery resulted in a transient increase of lipid peroxidation but not in total plasma antioxidative capacity in dairy cows (Mudron et al., ). A culmination of the TBARS was seen within 2 hr after the abomasal replacement, that is within a very short time period after stress.…”

“…It has been demonstrated that a number of manipulations, including transport (Earley et al., ), feed deprivation (Fürll, Kirbach, & Knobloch, ), therapeutic manipulation (Nakao, Sato, Moriyoshi, & Kawata, ) and surgery (Bergamasco et al., ; Fisher et al., ), increased secretion of cortisol from the adrenal cortex in cattle. Several studies demonstrated that the stress reaction has enhancing effects on free radical production, thus contributing to an increased lipid peroxidation in animals (Kreinhoff, Elmadfa, Salomon, & Weidler, ; Mudron, Herzog, Höltershinken, & Rehage, ). Although stress reactions are organized to protect the homoeostatic state of animals, they contain elements that may either enhance or diminish susceptibility to the disease process; in many instances, however, stress reactions themselves may induce pathologic change (Breazile, ).…”