Effects of a time varying strong magnetic field on release of cytosolic free Ca²⁺ from intracellular stores in cultured bovine adrenal chromaffin cells

Abstract

This study was made to explain the mechanisms for the effects of exposure to a time varying 1.51 T magnetic field on the intracellular Ca(2+) signaling pathway. The exposure inhibited an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in bovine chromaffin cells induced by addition of bradykinin (BK) to a Ca(2+) free medium. The exposure did not change BK induced production of inositol 1,4,5-trisphosphate (IP(3)). [Ca(2+)](i) was markedly increased in IP(3) loaded cells, and this increase was inhibited by the magnetic field exposure. A similar increase in [Ca(2+)](i) by other drugs, which stimulated Ca(2+) release from intracellular Ca(2+) stores, was again inhibited by the same exposure. However, transmembrane Ca(2+) fluxes caused in the presence of thapsigargin were not inhibited by the magnetic field exposure in a Ca(2+) containing medium. Inhibition of the BK induced increase in [Ca(2+)](i) by the exposure for 30 min was mostly recovered 1 h after exposure ended. Our results reveal that the magnetic field exposure inhibits Ca(2+) release from intracellular Ca(2+) stores, but that BK bindings to BK receptors of the cell membrane and intracellular inositol IP(3) production are not influenced.

The effect of pulsed electromagnetic fields on secondary skin wound healing: An experimental study

“…Pulsed electromagnetic fields (PEMFs) are capable in altering the structure of the cell membranes and thus diversify the permeability of different ion channels and the potential of the cellular membranes. Both phenomena are important of cellular functions [Blackman et al, 1980;Walleczek and Liburdy, 1990;Ikehara et al, 2002], such as the production of chemical energy in the form of adenosine triphosphate (ATP) and the variance of intracellular free calcium levels, which is a second type universal intermediate ion [Carafoli, 2004]. They may also conserve the normal electrochemical gradient of cells, a necessary condition for ATP production, which may be lowered by ischemia or trauma.…”

Effects of pulsed electromagnetic fields on benign prostate hyperplasia

“…EMFs may increase intracellular Ca 2? concentrations in various types of cells [22,23] inducing thus apoptosis also on prostate cancer cells [24]. Radiofrequency fields have been shown to exert apoptotic effects on malignant cell lines [2][3][4].…”

Effects of 50Hz Homogeneous Ferromagnetic Field on the Concentrations of Intracellular Calcium-ion of SNU Cells and Lymphma Cells In Vitro

“…Study revealed that Calcium-ion could adjust many species physiology actions as one of the second messenger, it also take part in regulation of proliferation, excretion and migration of the cells after exposure on the magnetic field [3]. There were reports that the concentration of the intracellular Calcium-ion or the calcium-signal increased after the exposure of the magnetic field [4], [5], on the contrary, other reports suggest that the concentration of the intracellular Calcium-ion or the calcium-signal decreased after the exposure of the magnetic field [6], [7], some reports suggest that magnetic field have no effects on the concentration of the Calcium-ion [8], [9]. All of the above reports showed that the effects of magnetic field on the concentration of intracellular Calcium-ion were complicated, it has relation to the frequency and the intensity of the magnetic field, the time exposure on the magnetic field and the type of the cells.…”