Effects of a Pituitary Homotransplant and Thyroxine on Body and Mammary Growth in Immature Hypophysectomized Rats

Meites, Joseph; Kragt, Clifford L.

doi:10.1210/endo-75-4-565

Cited by 39 publications

(15 citation statements)

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“…The results confirm the conclusion of previous work that female dwarf mice require prolactin (luteotrophin) to reproduce (Bartke, 1965b (Hurst & Turner, 1942) and in the normal female the initiation of milk production may be due to the increased prolactin secretion in late pregnancy and early lactation (Averill, 1965 Hertz, 1959;Meites & Kragt, 1964); (3) possible partial species specificity of sheep prolactin ; and (4) using males from a different stock. The relevance of (1) is further indicated by the inferior reproductive performance of females given prolactin once daily in which the fluctuation of the hormone blood level was still higher, while the influence of (4) seems very unlikely since the ICR Albino stock is noted for high fertility and large litter size (average thirteen).…”

Section: Discussionsupporting

confidence: 89%

Reproduction of Female Dwarf Mice Treated With Prolactin

Bartke¹

1966

Reproduction

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Summary. Genetically dwarf female mice are sterile but reproduction and lactation can be induced with prolactin-producing heterotopic pituitary homografts. To test if prolactin deficiency is the sole cause of the sterility, female dwarf mice were treated with purified prolactin.Dwarf (dwdw) and Ames dwarf (dfdf) mice in which the vaginae opened spontaneously were mated to non-dwarf males and injected with sheep prolactin starting on the day of plug formation. Pregnancy, delivery and lactation were obtained in both kinds of dwarf mouse indicating that, in the stocks used, the addition of prolactin alone is sufficient for reproduction.

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Section: Discussionsupporting

confidence: 89%

Reproduction of Female Dwarf Mice Treated With Prolactin

Bartke¹

1966

Reproduction

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“…Since that time, attempts at documenting growth recovery by hetero tropic hypophysial transplants in hypox rats have met with limited success [6,7,10,13,26].…”

Section: Introductionmentioning

confidence: 99%

Pituitary Hollow Fiber Units in vivo and in vitro

Hymer¹,

Wilbur

Page

et al. 1981

Neuroendocrinology

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A new technique is described for the study of mammalian pituitary cells in vivo and in vitro. The method involves encapsulation of freshly trypsinized rat, sheep or human pituitary cells in XM-50 Amicon hollow fibers followed by their intracranial implantation into hypophysectomized rats. These pituitary fiber units promoted recipient growth for ∼3 weeks before weight gains plateaued. Body composition analyses showed that significant quantities of protein, fat and ash accounted for the weight gain. Morphological study of the capsule contents 7–39 days postimplantation revealed the presence of intact somatotrophs and corticotrophs. The hollow fibers may provide an immunologically privileged site by virtue of the fact that the 50,000 dalton pores making up the lumen surface permit pituitary hormones to diffuse from the capsule, but theoretically do not permit immunoglobulin molecules to penetrate the capsule. Growth of hypox rats receiving capsules containing allogeneic rat pituitary cells, sheep cells or pieces of human postmortem pituitary support this concept. Furthermore, rats implanted with human PRL adenoma cells had detectable quantities of circulating hPRL 100 days post-implantation. It is suggested that the pituitary hollow fiber units function when they come in contact with a ventricular surface of a hypox animal. With these units, it will be possible to study function of the same group of pituitary cells in vitro and in vivo.

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“…The fact that thyroid hormones play a fundamental role in the morphological development of mammary glands is beyond question although the exact nature of that role is still debated. Growth-promoting, inhibitory and regulatory roles have been ascribed to these hormones by various workers (7)(8)(9)(10)(11). The régula-tory role is mainly mediated via alterations in the binding of prolactin and it has been postulated that prolactin acts by increasing tire number of epithelial cells and that thyroid hormones modulate the number of prolactin receptors per cell (12,13).…”

Section: Introductionmentioning

confidence: 99%

Prolactin, Thyrotrophin Interaction in the Regulation of Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase Activities in Rat Mammary Glands

Deshpande¹,

Mitchell²

1980

Horm Res

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The roles of prolactin and thyrotrophin (TSH) in the regulation of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH) activities in rat mammary glands were investigated by the administration of thyroid hormonereleasing hormone (TRH), bromocriptine, prolactin, TSH and triiodo-1-thyronine (T₃). TRH failed to induce changes in the activities of these enzymes in glands from intact animals but the releasing hormone significantly increased the activities of both the enzymes in the glands of ovariectomized and adrenalectomized animals. Bromocriptine administration had no effect on the activities in both, intact and ovariectomized-adrenalectomized animals. Administration of ovine prolactin to hypophysectomized rats did not affect the activities of these enzymes. On the other hand treatment with TSH resulted in significant increases in the activities. Similarly, administration of T₃ to these animals resulted in changes similar to those observed after TSH administration. Combined administration of prolactin and TSH showed that prolactin is capable of partially inhibiting the TSH-induced increases. It is concluded that TSH is involved in the channelling of substrates into the pathways of nucleic acid synthesis and prolactin probably plays a regulatory role in this process.

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Effects of a Pituitary Homotransplant and Thyroxine on Body and Mammary Growth in Immature Hypophysectomized Rats

Cited by 39 publications

References 0 publications

Reproduction of Female Dwarf Mice Treated With Prolactin

Reproduction of Female Dwarf Mice Treated With Prolactin

Pituitary Hollow Fiber Units in vivo and in vitro

Prolactin, Thyrotrophin Interaction in the Regulation of Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase Activities in Rat Mammary Glands

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