Effects of a New Nano-Silver Fluoride-Containing Dentifrice on Demineralization of Enamel andStreptococcus mutansAdhesion and Acidogenicity

Abstract

An experimental dentifrice containing nano-silver fluoride (NSF) and a sodium fluoride (NaF) toothpaste were tested in vitro, against S. mutans, to evaluate the minimal inhibitory concentration (MIC), minimal bactericidal concentration (MBC), antiadherence, antiacid, enamel microhardness, and OCT. The microdilution technique was used to determine the MIC and MBC. Fragments of deciduous enamel were treated with dentifrice slurries, containing bacterial suspension and PBS-treated saliva. The quantification of the microorganisms that adhered to the enamel was determined after 24 hours of incubation, and media pH readings were performed after 2 hours and 24 hours. Deciduous teeth were evaluated for microhardness and OCT during 14 days of pH cycling. Data were statistically analyzed using Student's t-test, Mann–Whitney U test, ANOVA, and Tukey tests at 5% of significance. Dentifrices containing NSF presented a lower MIC and higher statistically significant results compared to NaF dentifrices with respect to preventing bacterial adhesion and pH decreases. NSF and NaF dentifrices showed the same ability to avoid enamel demineralization corroborated by the OCT images. The NSF formulation had a better antibacterial effect compared to NaF dentifrices and similar action on the demineralization of enamel indicating their potential effectiveness to prevent caries.

Antibacterial and Antifungal Activities of PMMAs Implanted Fluorine and/or Silver Ions by Plasma-Based Ion Implantation with Argon

“…In this study, there was no antibacterial activity against S. mutans in the Ar/F group implanted with F ions alone. Several studies have reported the use of F alone or a combination of F and Ag for dental materials to obtain an anti-cariogenic effect [ 38 , 39 ]. Lou et al [ 38 ] evaluated the antimicrobial effects of Ag and F agents against S. mutans , Lactobacillus acidophilus , and Actinomyces naeslundii and identified the components that are effective against these bacteria.…”

“…The conclusion was that Ag ions appear to be the principal antibacterial agent at both high and low concentrations, whereas F ions only have antibacterial effects at high concentrations. In another study [ 39 ], an experimental dentifrice containing nano-silver fluoride (NSF) with AgNP and a sodium fluoride (NaF) toothpaste were tested in vitro to evaluate the antibacterial property against S. mutans . It concluded that NSF, which includes both Ag and F, had a better antibacterial effect than NaF, Ag excluded.…”

The Influence of Type 1 Diabetes Mellitus on Dental Caries and Salivary Composition

“…Increasing the level of glucose in saliva affects the activity of microorganisms. Streptococcus mutans and Lactobacillus are considered to be related to caries and are the most cariogenic bacteria [ 11 ] because they have the ability to create a low pH environment and progression of caries [ 12 ]. Research studies show that Streptococcus mutans and Lactobacillus found in stimulated saliva explain better the development of caries than Streptococcus mutans and Lactobacillus found in plaque [ 13 , 14 ].…”

Evaluation of the Anticariogenic Effect of Nano Silver Fluoride on Demineralization of Enamel in Primary Teeth (An in Vitro Study)

“…The size and morphology of AgNPs was characterized using field emission transmission electron microscope (TEM) (JEOL JEM-2100F) (3,23). The electron micrographs showed that most of the particles exhibited a spherical shape and more than 50% of the detected particles' size ranged from 13nm to 16 nm ( Figure 2).…”