Effects of a membrane-permeable radical scavenger, Tempol, on healing of colonic anastomoses in the cecal ligation and puncture model of polymicrobial sepsis in rats

Aytekin, Faruk Önder; Teke, Zafer; Aydın, Çiğdem; Kabay, Burhan; Yenisey, Çiğdem; Saçar, Suzan; Demir, Ece; Tekin, Koray

doi:10.1016/j.amjsurg.2006.06.035

Cited by 18 publications

(12 citation statements)

References 41 publications

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“…35 Tempol was also found to reestablish GSH stores in peri-anastomotic colonic tissue in experimental polymicrobial sepsis. 36 In the present study, the reduction of GSH in ileal tissue specimens was most likely a result of oxidant injury, whereas we attributed the restoration of GSH in the ileal tissues of Tempol-treated animals to the antioxidant features of Tempol. 47,48 In conclusion, this study clearly demonstrates that Tempol prevents the detrimental effects of I/R injury on the intestinal tissue in a rat model of SMA occlusion.…”

Section: Discussioncontrasting

confidence: 48%

“…The dose of Tempol used in this study was based on what previous studies found to have effi cacy without side effects in models of intestinal I/R injury. 35,36 There is limited evidence of the potential side effects of Tempol. Evidence shows that while short time periods of exposure to Tempol can be protective against ischemia and reperfusion, long-term exposure can be potentially highly toxic.…”

Section: Discussionmentioning

confidence: 98%

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Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion

et al. 2009

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Section: Discussioncontrasting

confidence: 48%

Section: Discussionmentioning

confidence: 98%

Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion

et al. 2009

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“…Besides the mentioned factors, peritonitis is an important factor that can alter the cicatrization of anastomosis in bowels [19][20][21][22][23][24][25][26][27][28][29] .…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

et al. 2010

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Purpose:To analyze the effects of hyperbaric oxygen therapy (HBO) on the mechanical resistance of anastomosis performed in rats' distal colon presenting peritonitis induced by ligature and cecal punction using the Total Energy Rupture biomechanical test (ETR). Methods: It was used 45 rats divided into three groups of 15 animals each. In Control Group (CG), it was performed anastomosis in distal colon without peritonitis. In Peritonitis Group (PG), it was performed anastomosis six hours after the induction of peritonitis by ligature and cecal punction. In Hyperbaric Chamber Group (HCG), it was performed six hours after the induction of peritonitis by ligature and cecal punction. The animals on CG and PG were kept at place air while the animals on HCG were placed on an experimental hyperbaric chamber in order to inhale oxygen at 100%, two absolute atmospheres, for 120 minutes, for four consecutive days. Euthanasia took place on the fifth day of the experiment. All the animals underwent to Total Energy Rupture Biomechanical Resistance test (ETR). Total Energy Rupture was defined as the necessary internal energy stored up to promote the colon rupture after an external traction force imposition. Results: The Peritonitis Group presents smaller average ETR than Control Group. There was no statistical difference between Peritonitis Group and Hyberbaric Chamber Group. Conclusion: Hyperbaric oxygen therapy did not alter the mechanical resistance of anastomosis performed in distal colon of rats under the presence of peritonitis induced by ligature and cecal puncture. Key words: Anastomosis, Surgical. Colon. Hyperbaric Oxygenation. Peritonitis. Rats. RESUMO Objetivo:Analisar o efeito da oxigenoterapia hiperbárica (HBO) sobre a resistência mecânica de anastomoses realizadas em cólon distal de ratos na presença de peritonite induzida por ligadura e punção cecal utilizando o teste biomecânico de Energia Total de Ruptura (ETR). Métodos: Foram utilizados 45 ratos distribuídos em três grupos de 15 animais. No Grupo Controle (GC), realizou-se anastomose no cólon distal sem peritonite. No Grupo Peritonite (GP), realizou-se anastomose seis horas após a indução da peritonite por ligadura e punção cecal. No Grupo Câmara Hiperbárica (GCH), realizou-se anastomose seis horas após a indução da peritonite por ligadura e punção cecal. Os animais dos GC e GP foram mantidos em ar ambiente. Os animais do GCH foram colocados em uma câmara hiperbárica experimental para inalarem oxigênio a 100%, a duas atmosferas absolutas, durante 120 minutos, por quatro dias consecutivos. A eutanásia ocorreu no quinto dia do experimento. Todos os animais foram submetidos ao Teste de Resistência Biomecânico Energia Total de Ruptura (ETR). A Energia Total de Ruptura foi definida como a energia interna acumulada necessária para promover o rompimento do cólon após a imposição de uma força externa de tração. Resultados: O Grupo Peritonite apresentou menor média de ETR que o Grupo Controle. Não houve diferença estatística entre o Grupo Peritonite e o Grupo C...

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“…Proteins may also be targets of ROS; for example, oxidation disrupt protein receptors, affects enzymatic functions and interrupts signal transduction pathways 17,19 . The reduction of lipid peroxidation and increased GSH levels in the tissue of colon anastomosis in rats is associated with the increased hydroxyproline content of the tissues, which can increase the burst pressure of the anastomosis 20 . Malonaldehyde (MDA), which is a product of lipid peroxidation, shows increased levels in delayed wound healing, resulting from the use of hydrocortisone in mice compared with control mice group 21 .…”

Section: Discussionmentioning

confidence: 99%

Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats

Oliveira

et al. 2014

Acta Cir. Bras.

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PURPOSE:We evaluated the hypothesis that induced perioperative hypothermia (32 ± 1ºC) affects the redox balance in the tissue of colonic anastomosis in rats by modifying biochemical enzymatic and non-enzymatic markers related to oxidative stress. METHODS:Forty-eight male Wistar rats were randomly divided into eight experimental groups of six animals each and underwent laparotomy, sigmoid section and immediate anastomosis. Four groups were operated under normothermia (36 ± 1ºC), and the other four under hypothermia (32 ± 1ºC). The animals were reoperated on days 3, 7 and 14 postoperatively, and two groups underwent SHAM at 3 days. From the scar tissue samples, the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was evaluated, and the levels of non-enzymatic markers of oxidative stress, such as reduced glutathione (GSH) and lipid peroxidation, were measured by the thiobarbituric acid reactive substances (TBARS) assay. The means were compared between groups corresponding to each day of sampling and euthanasia. RESULTS:The hypothermic groups showed a significant reduction on the activity of SOD on day 7 postoperatively, on the activity of CAT on days 7 and 14 postoperatively and on the levels of GSH on day 7 postoperatively. The level of lipid peroxidation was increased in the hypothermia group on day 7 postoperatively and decreased on day 14 compared with the normothermic groups. CONCLUSION:Perioperative hypothermia reduced the activity of the antioxidant enzymes catalase and superoxide dismutase, glutathione levels and increased lipid peroxidation in the scar tissue of colonic anastomoses in rats.

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Effects of a membrane-permeable radical scavenger, Tempol, on healing of colonic anastomoses in the cecal ligation and puncture model of polymicrobial sepsis in rats

Cited by 18 publications

References 41 publications

Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion

Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion

Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats

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