Effects of a lecithin and catalase containing semen extender and a second dilution with different enhancing buffers on the quality of cold-stored canine spermatozoa

Kmenta, I.; Strohmayer, Carina; Müller-Schlösser, F; Schäfer-Somi, Sabine

doi:10.1016/j.theriogenology.2010.11.018

Cited by 37 publications

(33 citation statements)

References 41 publications

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“…Studies have suggested that 200 and 400 U/ml CAT are equally effective in preserving motility and DNA integrity of frozen‐thawed boar sperm (Roca et al., ). In dogs, an extender concentration of 150 U/ml CAT was reported to preserve progressive motility and viability after 8 days in cold storage (Kmenta, Strohmayer, Müller‐Schlösser, & Schäfer‐Somi, ). Catalase at 100 U/ml was not shown to prevent the loss of motility in human spermatozoa (Calamera, Fernandez, Buffone, Acosta, & Doncel, ), but 200 U/ml was associated with better post‐thaw progressive motility, spermatozoan viability and DNA integrity than standard extender alone (Moubasher, El Din, Ali, El‐sherif, & Gaber, ).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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High levels of reactive oxygen species (ROS), which may be associated with reduced sperm quality, can be detected during cryopreservation of sperm of some species. Our objective was to investigate whether the addition of antioxidants to cryopreservation extenders influenced post-thaw sperm characteristics and fertility in Acipenser dabryanus, Acipenser sinensis and Acipenser baerii. Prior to freezing, sperm samples were diluted with a base extender as control or in extender supplemented with catalase (CAT), glutathione (GSH), cysteine (NAC), ascorbic acid (VC) or their paired combinations. Protective concentrations of CAT, GSH and VC in the three species were 25 U/ml, 0.25-0.5 mg/ml and 0.5 mg/ml, respectively. Cysteine showed no protective effect against ROS. The addition of CAT, GSH and VC positively affected either acrosome or membrane integrity of post-thawed sperm in the three species, as well as spermatozoan motility in A. sinensis. The combination of antioxidants did not show a positive synergistic effect. This study suggested that the use of antioxidants in the cryopreservation of sturgeon sperm has potential to decrease intracellular ROS, and consequently preserve acrosome and membrane integrity, as well as spermatozoan motility.

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Section: Discussionmentioning

confidence: 99%

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

et al. 2017

Reprod Domestic Animals

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“…() used 0.04% soya lecithin from Solae company for cooling while Kmenta et al. () used 0.8% soya lecithin from Sigma for cooling of dog semen. Cryopreservation of dog semen in an 1.5% lecithin extender has been described by Hidalgo et al.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk

Axnér

Lagerson

2016

Reprod Domestic Animals

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Egg yolk is usually included in extenders used for preservation of dog semen. Lecithin is an interesting animal-protein free alternative to egg yolk for semen preservation. The aim of our study was to evaluate soya bean lecithin for cryopreservation of dog semen. Five ejaculate replicates were divided in three equal parts, centrifuged and each pellet diluted with one of the three Tris-based extenders containing 20% egg yolk, 1% soya bean lecithin or 2% soya bean lecithin. Extended semen was loaded in 0.5-ml straws, cooled and diluted a second time and frozen in liquid nitrogen vapours. Sperm motility parameters (CASA), acrosome integrity (FITC-PNA/PI) and sperm membrane integrity (C-FDA) were evaluated 5 min post-thaw and after 2 and 4 h of incubation. Total motility was significantly better in the egg yolk extender than in any of the lecithin-based extender and was better in the 1% lecithin extender than in the 2% lecithin extender. Sperm membrane integrity was significantly better in the egg yolk extender than in any of the lecithin-based extenders but did not differ significantly between the 1% and 2% lecithin extenders. Acrosome integrity was significantly better in the egg yolk extender than in the 2% lecithin extender but did not differ between the egg yolk extender and the 1% lecithin extender or between the two lecithin extenders. In conclusion, egg yolk was superior to lecithin in our study. The extender with 1% lecithin preserved sperm motility better than the extender with 2% lecithin.

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“…These damages can be decreased by adding lipids, in the form of egg yolk, to the sperm prior to cooling and freezing. Egg yolk is the main membrane protective agent in semen extenders [6] . The main disadvantage is of having a protective agent of an animal source in extenders used for short term preservation or cryopreservation is the risk of disease transportation [7] and microbial contamination which allows production of endotoxins [8] .…”

Section: Introductionmentioning

confidence: 99%

7-dehidrokolesterol ve Kolesterol İle Doyurulmuş Siklodekstrinlerin Kısa Süreli Saklama Süresince Boğa Spermasının Motilitesine Etkisi

̇İnanç¹,

Tekin²,

Olğaç³

et al. 2017

Kafkas Univ Vet Fak Derg

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The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4°C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x10 6 group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4°C. Keywords: Bull sperm, Cholesterol, Motility, Short term storage, 7-dehydrocholesterol 7-dehidrokolesterol ve Kolesterol İle Doyurulmuş Siklodekstrinlerin Kısa Süreli Saklama Süresince Boğa Spermasının Motilitesine Etkisi ÖzetBu çalışma, kısa süreli saklama süresince (+4°C) 7-dehidrokolesterol doyurulmuş siklodekstrin (7-DHCLC) ve kolesterol ile doyurulmuş siklodekstrinin (CLC) boğa spermasının motilitesinin korunmasındaki etkisinin değerlendirilmesi için yürütülmüştür. Alınan ejekulatlar birleştirilerek 7 gruba ayrıldı, bu gruplardan biri control (C), üçü 7-DHCLC'nin diğer üçü ise CLC'nin farklı konsantrasyonlarını içeren sulandırıcılarla sulandırıldı. Sulandırılan sperma +4°C'ye transfer edildilerek saklandı ve sperma motilitesi üç gün boyunca bilgisayar destekli sperm analiz cihazında (CASA) değerlendirdi. Motilitenin tüm gruplarda azaldığı görüldü fakat, 7-DHCLC ve CLC gruplarında motilite azalmasının daha yavaş olduğu tespit edildi. Fakat, CLC 1.5 mg/120×10 6 grubunun spermanın kısa süreli saklanmasında motiliteyi koruyan en iyi grup olduğu belirlendi. Sonuç olarak, sperma sulandırıcısına değişik oranlarda 7DHCLC ve CLC'nin eklenmesi +4°C'de boğa sperma motilitesini üç gün boyunca koruduğu tespit edildi.

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Effects of a lecithin and catalase containing semen extender and a second dilution with different enhancing buffers on the quality of cold-stored canine spermatozoa

Cited by 37 publications

References 41 publications

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Antioxidant supplementation, effect on post‐thaw spermatozoan function in three sturgeon species

Cryopreservation of Dog Semen in a Tris Extender with 1% or 2% Soya Bean Lecithin as a Replacement of Egg Yolk

7-dehidrokolesterol ve Kolesterol İle Doyurulmuş Siklodekstrinlerin Kısa Süreli Saklama Süresince Boğa Spermasının Motilitesine Etkisi

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