Effects of a High Fat Diet and Voluntary Wheel Running Exercise on Cidea and Cidec Expression in Liver and Adipose Tissue of Mice

Reynolds, Thomas H.; Banerjee, Sutapa; Sharma, Vishva Mitra; Jp, Donohue; Couldwell, Sandrine; Sosinsky, Alexandra Z.; Frulla, Ashton; Robinson, Allegra; Puri, Vishwajeet

doi:10.1371/journal.pone.0130259

Cited by 25 publications

(18 citation statements)

References 45 publications

(60 reference statements)

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“…Elevated CIDEA expression and diminished PRKACA , which phosphorylates and activates HSL, suggests that lipolysis was inhibited in tumour-bearing animals. This finding is consistent with previous studies reporting larger lipid droplets, diminished lipolysis and elevated triglyceride storage capacity of adipose tissue concurrent with CIDEA overexpression in adipose tissue [ 23 , 24 ]. It should be noted that depending on the time (stage) of tumour growth and type of adipose tissue, previous studies have reported either smaller or larger adipocytes in tumour-bearing animals [ 25 , 26 ].…”

Section: Discussionsupporting

confidence: 93%

Chemotherapy diminishes lipid storage capacity of adipose tissue in a preclinical model of colon cancer

et al. 2017

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BackgroundAccelerated loss of adipose tissue in cancer is associated with shorter survival, and reduced quality of life. Evidence is emerging suggesting tumour association with alterations in adipose tissue, but much less is known about drug-related mechanisms contributing to adipose atrophy. Identification of mechanisms by which tumour and cancer treatments, such as chemotherapy, affect adipose tissue are required to develop appropriate therapeutic interventions to prevent fat depletion in cancer. This pre-clinical study aimed to assess alterations in adipose tissue during the clinical course of cancer.Methods Fischer 344 rats bearing the Ward colorectal tumour were euthanized before chemotherapy, after 1- cycle, or 2-cycles of a combination chemotherapy consisting of Irinotecan (CPT-11) combined with 5-fluorouracil (5-FU), which recapitulates first line treatment for human colorectal cancer. Periuterine adipose tissue was isolated. Healthy rats served as a reference group. Histological analysis (hematoxylin and eosin), Real-time PCR (TaqMan) and proteomic analysis (LC-MS/MS) were performed.ResultsLarger adipocytes (3993.7 ± 52.6 μm2) in tumour-bearing animals compared to the reference group (3227.7 ± 36.7 μm2; p < 0.001) was associated with reduced expression of proteins involved in mitochondrial fatty acid oxidation. The presence of a tumour has a significant effect on phospholipid but not triglyceride fatty acid composition. There were greater proportions of saturated fatty acids concurrent with lower monounsaturated fatty acids within the PL fraction of adipocytes in tumour-bearing animals. Chemotherapy treatment decreased the size of adipocytes (2243.9 ± 30.4 μm2; p < 0.001) and led to depletion of n-3 polyunsaturated fatty acids in adipose tissue triglyceride. Evaluation of the proteome profile revealed decreased expression of proteins involved in ATP generation, β-oxidation, and lipogenesis. Overall, adipose tissue may not be able to efficiently oxidize fatty acids to provide energy to maintain energy demanding pathways like lipogenesis inside the tissue.ConclusionsIn conclusion, metabolic adaptations to mitochondrial impairment may contribute to diminished lipid storage capacity of adipose tissue following chemotherapy delivery.Electronic supplementary materialThe online version of this article (10.1186/s12944-017-0638-8) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 93%

Chemotherapy diminishes lipid storage capacity of adipose tissue in a preclinical model of colon cancer

et al. 2017

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“…Cidea and Cidec, localized to lipid droplets and endoplasmic reticulum, are predominantly expressed in adipose tissues and liver, and have been shown to control lipid metabolism in adipocytes and hepatocytes through influencing lipogenesis or lipid droplet formation and to play important roles in the development of metabolic disorders (Gong et al., ; Yonezawa, Kurata, Kimura, & Inoko, ). Reynolds found that a HFD promoted a significant increase in Cidea and Cidec mRNA levels in adipose tissue and liver, and the overexpression of CIDE proteins increased TG accumulation (Reynolds et al., ). Recently, some studies also showed that Cidea and Cidec genes are highly expressed in the fatty livers of aged mice, of type 2 diabetic mice and ob/ob mice (Kelder et al., ; Matsusue et al., ).…”

Section: Discussionmentioning

confidence: 99%

Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome

Peng

Huang²,

Ruan

et al. 2018

Animal Science Journal

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Cidea and Cidec are two members of Cell death-inducing DNA fragmentation factoralpha-like effector family proteins, which could be involved in lipid or fat metabolism.To better understand the roles of Cidea and Cidec in fatty liver hemorrhagic syndrome (FLHS), 150 healthy 155-day-old Hyline Brown laying hens were randomly divided into control group (fed with basic diet) and experimental group (fed with high-energy low-protein [HELP] diet). Analysis of the liver by tissue sectioning and hematoxylin and eosin staining showed that the HELP diet induced micro-vesicular steatosis in laying hens. Subsequently, based on the liver color scores and the range of lipid accumulation observed in histological examination, we classified livers with <50% vacuolization as mild FLHS and >50% as severe FLHS. The results showed that the levels of Cidea and Cidec mRNA expression were markedly elevated in the liver and adipose tissues with FLHS and the levels of Cidea and Cidec mRNA expression in the liver with severe FLHS were significantly higher than that in the liver with mild FLHS. Thus, the present study revealed that the Cidea and Cidec genes may be involved in pathways of FLHS formation. K E Y W O R D SCidea, Cidec, fat metabolism, fatty liver hemorrhagic syndrome, laying hen

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“…Previous studies showed that exercise training with voluntary wheel reduced body weight and adiposity in diet-induced obese mice as well as normal diet treated lean mice ( Reynolds et al, 2015 ). Consistently, body weight was significantly decreased with the reduction of WAT weights in Exe after 6 weeks of voluntary wheel running in this study ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Exercise training regulates angiogenic gene expression in white adipose tissue

Lee¹

2018

J Exerc Rehabil

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White adipose tissue (WAT) expansion is associated with angiogenesis. Although, activation of lipolysis by exercise induces adipocyte hypotrophy and reduction of fat mass, it is poorly understood whether exercise regulates angiogenesis by altering angiogenic gene expression in WAT. Therefore, the purpose of this study was to evaluate the effect of 6 weeks voluntary wheel running exercise on angiogenic gene expression in adipose tissues. Male C57BL/6J mice performed voluntary wheel running for 6 weeks. At 24 hr after the last exercise training, tibialis anterior (TA), soleus (Sol), epididymal WAT (eWAT), inguinal WAT (iWAT), and brown adipose tissue (BAT) were isolated and then the expressions of vascular endothelial growth factor A (VEGFA), angiopoietin1 (Ang1), Ang2, platelet-derived growth factor B (PDGF-B) and their corresponding receptors were analyzed by reverse transcription-polymerase chain reaction. In skeletal muscles, VEGFA expression was upregulated in TA and Sol and PDDGF-B expression was increased in Sol after exercise training. In eWAT, the expressions of VEGFA and Flk-1 were dramatically downregulated, whereas Ang2 and PDGFRβ was upregulated after exercise training. In iWAT, VEGF expression was increased with the downregulation of Ang1. However, there was no alteration of any of these genes in BAT. These results suggest that angiogenic gene expression is altered by exercise training and voluntary wheel running regulates VEGFA, Ang1, and Ang2 expressions in a fat depot specific manner.

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Effects of a High Fat Diet and Voluntary Wheel Running Exercise on Cidea and Cidec Expression in Liver and Adipose Tissue of Mice

Cited by 25 publications

References 45 publications

Chemotherapy diminishes lipid storage capacity of adipose tissue in a preclinical model of colon cancer

Chemotherapy diminishes lipid storage capacity of adipose tissue in a preclinical model of colon cancer

Effects of a high energy and low protein diet on hepatic and plasma characteristics and Cidea and Cidec mRNA expression in liver and adipose tissue of laying hens with fatty liver hemorrhagic syndrome

Exercise training regulates angiogenic gene expression in white adipose tissue

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