Effects of a combination of an antibacterial agent (ofloxacin) and a collagenase inhibitor (FN-439) on the healing of rat periapical lesions

Anan, Hisashi; Matsumoto, Atsushi; Hamachi, Toshihiro; Yoshito, Yoshimine; Morita, Yukie; Maeda, Katsumasa

doi:10.1016/s0099-2399(96)80061-6

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…The rat model for evaluating the effect of intracanal medication involved in apical periodontitis is well established (Anan et al . 1996, Wagner et al . 2011).…”

Section: Discussionmentioning

confidence: 99%

“…The present findings are consistent with the features of periapical repair described in earlier studies, namely sparse inflammatory cell infiltration and intense fibroblastic proliferation (Anan et al . 1996, Dammaschke et al . 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The ROI for macrophage quantification was defined as the area enclosed by the XY line and the surface of the alveolar bone (Anan et al . 1996). The alveolar bone in this area was regarded as the ROI for the osteoclast quantification.…”

Section: Methodsmentioning

confidence: 99%

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The potential of a surface pre‐reacted glass root canal dressing for treating apical periodontitis in rats

et al. 2020

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AimTo evaluate the efficacy of a prototype root canal dressing containing surface pre‐reacted glass–ionomer (S‐PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2] was applied as a comparison in the healing process.MethodologyThe pulp chambers of the maxillary first molars in 64 male Wistar rats aged 16 weeks were opened to induce periapical lesions. After 28 days, the mesial canal of each tooth was prepared, irrigated with 2.5% sodium hypochlorite only (control group: irrigation) or followed by the respective dressing [Ca(OH)2 group, irrigation + Ca(OH)2; S‐PRG group, irrigation + S‐PRG] and restored with composite resin for 3 or 7 days (10/group). Four rats with healthy molars were used as blank controls. Descriptive analysis of the periapical radiographs, haematoxylin and eosin staining and immunohistochemical observation was performed 3 and 7 days after treatment. The periapical grey value, CD68 macrophages and osteoclasts (cathepsin‐K) were quantified and statistically analysed with Tukey’s honest significant difference test. A significant difference was achieved when P values were <0.05.ResultsS‐PRG and Ca(OH)2 dressings were associated with increased periapical grey values and inhibited osteoclast activity at 3 and 7 days; a significant difference in radiographic results and the number of osteoclasts was obtained at 3 and 7 days compared with the control group (P < 0.05). Reparative tissue was observed histologically in the space of the periapical resorbed necrotic area after S‐PRG and Ca(OH)2 treatment for 3 and 7 days. The number of macrophages was significantly decreased at 3 and 7 days in the S‐PRG and Ca(OH)2 specimens when compared with the controls (P < 0.05).ConclusionsIn a rat experimental model, the S‐PRG root canal dressing was comparable to Ca(OH)2 in promoting the healing of experimentally induced periapical lesions. S‐PRG paste has the potential to be used as an alternative intracanal dressing in teeth with apical periodontitis.

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“…The rat model for evaluating the effect of intracanal medication involved in apical periodontitis is well established (Anan et al . 1996, Wagner et al . 2011).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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The potential of a surface pre‐reacted glass root canal dressing for treating apical periodontitis in rats

et al. 2020

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“…In the current study, MMP activity was inhibited with FN-439, a commercially available peptidyl hyroxamic acid developed for its action on human MMPs (Odake et al, 1994). This compound is water soluble and retains its inhibitory activity in the presence of neutral proteinases, making it a good choice for the modulation of MMP activity in vivo (Kigasawa et al, 1995;Anan et al, 1996). We applied FN-439 at 30 min postlesion, followed by electrophysiological and electron microscopic analysis at 7 d survival.…”

Section: Introductionmentioning

confidence: 84%

Matrix Metalloproteinase Inhibition Alters Functional and Structural Correlates of Deafferentation-Induced Sprouting in the Dentate Gyrus

et al. 2003

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Molecules comprising the extracellular matrix (ECM), and the family of matrix metalloproteinases (MMPs) that regulate them, perform essential functions during neuroplasticity in both developing and adult nervous systems, including substrate guidance during neuritogenesis and the establishment of boundaries for axonal terminal fields. MMP proteolysis of ECM molecules may perform a permissive or inductive role in fiber remodeling and synaptogenesis initiated by deafferentation. This study examined functional and structural effects of MMP inhibition during the early phases of deafferentation-induced sprouting, characterizing components of the degeneration/proliferation cycle that may be dependent on MMP activity. Adult rats received unilateral lesions of the entorhinal cortex to induce collateral sprouting of the crossed temporodentate fiber pathway. This was followed by intraventricular infusion of the MMP inhibitor FN-439 (2.9 mg/kg) or saline vehicle. After 7 d postlesion, rats underwent in vivo electrophysiological recording or histological processing for electron microscopic analysis. Lesioned rats receiving vehicle exhibited normal sprouting and synaptogenesis, with the emergence of the capacity for long-term potentiation (LTP) within the sprouting pathway, and the successful clearance of degenerating terminals with subsequent synaptic proliferation. In contrast, lesioned rats receiving the MMP inhibitor failed to develop the capacity for LTP and showed persistent cellular debris. Current source density analysis also revealed an FN-439-induced disruption of the current sink, normally localized to the middle region of the granule cell dendrites, corresponding to the terminal field of the crossed temporodentate fibers. These results establish a role for MMP-dependent processes in the deafferentation/sprouting cycle.

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Healing of Apical Lesions: How Do They Heal, Why Does the Healing Take So Long, and Why Do Some Lesions Fail to Heal?

Metzger

Kfir

2014

Disinfection of Root Canal Systems

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Effects of a combination of an antibacterial agent (ofloxacin) and a collagenase inhibitor (FN-439) on the healing of rat periapical lesions

Cited by 7 publications

References 18 publications

The potential of a surface pre‐reacted glass root canal dressing for treating apical periodontitis in rats

The potential of a surface pre‐reacted glass root canal dressing for treating apical periodontitis in rats

Matrix Metalloproteinase Inhibition Alters Functional and Structural Correlates of Deafferentation-Induced Sprouting in the Dentate Gyrus

Healing of Apical Lesions: How Do They Heal, Why Does the Healing Take So Long, and Why Do Some Lesions Fail to Heal?

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