Effects of a bismuth salt on cell proliferation, endocytosis, and fine structure of Tetrahymena

Nilsson, Jytte R.

doi:10.1016/s0932-4739(96)80050-2

Cited by 16 publications

(28 citation statements)

References 28 publications

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“…MT is expected to increase greatly in Tetrahymena under the exposure to excess Zn due to its quick stress response and high metal-binding affinity (Nilsson 2003). In the present study, Zn was found to have a 127-and 31-fold induction effect on the TpMT-1 and TpMT-2 gene respectively.…”

Section: Article In Presssupporting

confidence: 49%

“…Although the physiological differences between the three Tetrahymena species may contribute to the varied transcription responses, the different mRNA level in our experiments may result from both the high Zn concentration and the time point (1 h) we chose to investigate. Nilsson (2003) showed that a high concentration of Zn was well tolerated by Tetrahymena. When excess Zn was present, there was an induced lag period for the cell proliferation followed by normal growth rate, suggesting that the high sublethal amounts of Zn had an initial negative effect but no adverse longtime effects.…”

Section: Article In Pressmentioning

confidence: 99%

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Cloning and Characterization of a New Multi-Stress Inducible Metallothionein Gene in Tetrahymena pyriformis

Miao

2006

Protist

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Section: Article In Presssupporting

confidence: 49%

Section: Article In Pressmentioning

confidence: 99%

Cloning and Characterization of a New Multi-Stress Inducible Metallothionein Gene in Tetrahymena pyriformis

Miao

2006

Protist

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“…Several authors reported the optimum growth of protozoa at pH 7.5±0.2 (Rehman et al, 2005;Madoni et al, 1996). Nilsson (2003) reported optimum growth of T. pyriformis at pH 7. The culture began to deplete at pH ≤ 6.5 and ≥ 8 in both Bold-basal salt medium and ATCC medium 357.…”

Section: Organism Day Amount Of Copper (µM) In the Mediummentioning

confidence: 99%

“…RT-1 showed maximum growth at 25°C in both media. Several authors have reported the growth of T. pyriformis at 28°C (Nilsson, 2003;Piccinni et al, 1994). It was stated that the multiplication rate was slightly lower at low temperatures (≈20°C) although at this temperature, the culture can be kept a bit longer as compared to higher temperatures (≈37ºC).…”

Section: Organism Day Amount Of Copper (µM) In the Mediummentioning

confidence: 99%

Characterization of copper resistant ciliates: Potential candidates for consortia of organisms used in bioremediation of wastewater

Chaudhry¹,

Shakoori²

2011

Afr. J. Biotechnol.

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Metals are environmental pollutants of major concern due to their ecological, sanitary and even economic consequences. Both prokaryotes and eukaryotes inhabiting such environments carry cellular systems that maintain the metal homeostasis. The ciliate protists tolerate elevated concentrations of metals, which are accumulated, bound to metallothioneins (MTs) peculiar to these organisms. Copper is one of such contaminant found in the wastewater of local industries. The concentrations of copper which caused 50% reduction (LC 50 ) in the cell population of Tetrahymena sp RT1, and two Euplotes spp. RE-1 and RE-2, isolated from the industrial waste, were found to be 60, 48 and 49 ppm, respectively, compared to those of the cultures without copper in the media. RT-1 showed significantly high tolerance to copper ions and could uptake 52.66% of the copper ions from the medium. The axenic culture of RT-1 could uptake 61.2% of copper from the medium compared to 68.41 and 59.16% by the ATCC culture of Tetrahymena thermophila and T. pyriformis, respectively. RT-1 tolerated about 500 µM copper in the medium without affecting its movement. This ciliate showed promise as a member of the consortium used for bioremediation of copper contaminated wastewater.

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“…Key worux amino acids, GC-C-IRMS, GC-MS, nitro- Cell culture has been used frequently as a test system for toxicity assessment in pharmacology (4,6,7) and ecotoxicology (8,9). By observing end points such as growth impairment (10), modification of motility (7,11), and inner and outer morphology (6,12,13) …”

mentioning

confidence: 99%

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

Arndt¹,

Hofmann²,

Gehre³

et al. 1998

Environmental Health Perspectives

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A pilot study was perfonned to examine the potential of stable isotope tiques for monitoring the impact of a harmfil substance on the cellular nitrogen metabolism in the ciliate species Tenra/mnayfornnis. After identical cultivation periods ofcontrol cals and toluene-exposed cells in a defined culture medium enriched with (guanidino5N2]L-argnine, a number of nitrogen-contaning pools were analyzech 1) quantity and 15N abundance of ammonia as the end product of nitrogen metabolism in the sytem; 2) pattem and 15N abundances of the protein-bound amino acids in thecells; 3) patten and 15N abundancesoffreeamino acids inthe clls; and 4) pattern and 15N abundances of the amino acids in the culture medium. In addition to 15N emission spectometry, a new gas chromatogrhybcombuston interace-iotope ratio mass spectrometry/mass spectrometry an cal stem was used. The production and 15N content of ammonia were higher in the toluene-exposed sysem by 30% and 43%, respectily, indig higher demination rates and greater argnie consumptont The toluene-exposed cells exhibited increased 15N abundances ofprotein-bound amino acids in alanine, aspartic acid, gluhtmic acid, and tyrosine. Furthermore, strctural anals revealed the presence of N0-acelarginine and pyrrolidonecarboxylic acid-compounds dtat had not previously been detcted in Tena4ym aprfni. Differences in the '5N-enrichment of fee amino acids were also evident. This new effect-monitoring stem designed to investigte the impact ofa pollut on protein metabolism by using a stable isotope-labeled cell cuture is a powerfil tool for environmentl medical rsach. Key worux amino acids, GC-C-IRMS, GC-MS, nitro- Cell culture has been used frequently as a test system for toxicity assessment in pharmacology (4,6,7) and ecotoxicology (8,9). By observing end points such as growth impairment (10), modification of motility (7,11), and inner and outer morphology (6,12,13)

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Effects of a bismuth salt on cell proliferation, endocytosis, and fine structure of Tetrahymena

Cited by 16 publications

References 28 publications

Cloning and Characterization of a New Multi-Stress Inducible Metallothionein Gene in Tetrahymena pyriformis

Cloning and Characterization of a New Multi-Stress Inducible Metallothionein Gene in Tetrahymena pyriformis

Characterization of copper resistant ciliates: Potential candidates for consortia of organisms used in bioremediation of wastewater

15 N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for Environmental Medical Research

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