Effects of a Bacteria-Based Probiotic on Subpopulations of Peripheral Leukocytes and Their Cytokine mRNA Expression in Calves

Qadis, Abdul Qadir; Goya, Satoru; Yatsu, Minoru; Yoshida, Yuuki; Ichijo, Toshihiro; Satō, Shigeru

doi:10.1292/jvms.13-0370

Cited by 19 publications

(9 citation statements)

References 35 publications

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“…2014a) and ruminal performance in cattle (Qadis et al . 2014b, 2014c). Steers that received probiotics containing both lactate‐utilizing Propionibacterium and lactate‐producing Enterococcus had higher ruminal concentrations of acetate, and the blood variables indicated a reduced risk of metabolic acidosis (Ghorbani et al .…”

Section: Pathophysiological Evaluation Of Sara By Continuous Monitorimentioning

confidence: 99%

Pathophysiological evaluation of subacute ruminal acidosis (SARA) by continuous ruminal pH monitoring

Satō

2015

Animal Science Journal

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Evaluation of the radio‐transmission pH‐measurement system for monitoring the ruminal pH and subacute ruminal acidosis (SARA) in cattle is described. This is done in order to reveal the possible application of this system for detection and pathophysiological research of SARA by continuous ruminal pH measurement. The possibility of using this system for assessment of the ruminal pH in SARA cattle, and the presence of negative correlation between the ruminal pH and ruminal temperature in heathy and SARA cattle were determined. In addition, the 16S rRNA gene pyrosequencing analysis showed that the ruminal microbial community was simpler in SARA cattle, and the bacterial numbers in SARA cattle were lower than those in healthy hay‐fed cattle. Concentrate feeding might have reduced the diversity of the ruminal microbial community. Changes in the ruminal microbial community of SARA cattle might be related to the changes in ruminal pH followed by the decrease in the number of some bacteria. Continuous monitoring of the ruminal pH using the radio‐transmission pH‐measurement system would be applied for detection and prevention of SARA in the field and pathophysiological research of SARA, including ruminal zymology and bacteriology, which have been determined previously by sampling of the ruminal fluid and measuring of ruminal pH.

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Section: Pathophysiological Evaluation Of Sara By Continuous Monitorimentioning

confidence: 99%

Pathophysiological evaluation of subacute ruminal acidosis (SARA) by continuous ruminal pH monitoring

Satō

2015

Animal Science Journal

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“…The intestine of a newborn calf is sterile and colonization of the gastrointestinal (GI) tract begins immediately after birth [ 38 ]. Therefore, appropriate development of the GI tract microbiota in the early weeks of life is crucial for a functional immune system [ 26 ]. The intestinal microbiota is affected several factors.…”

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Effects of dietary feed supplementation of heat-treated <i>Lactobacillus sakei</i> HS-1 on the health status, blood parameters, and fecal microbes of Japanese Black calves

et al. 2020

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This study investigated the effect of heat-killed Lactobacillus sakei HS-1 (HK-LS HS-1) on the health and fecal bacteriological change of suckling Japanese Black calves as a supplement in milk replacers. Twelve calves were separated from dams to calf-hatch after calving for milk replacers feeding. They were randomly assigned to an HK-LS HS-1 supplement or a control without HK-LS HS-1 group in milk replacers. HK-LS HS-1 was administered from separation day to 3 weeks. Blood and fecal samples were examined. Two calves with a haptoglobin concentration of >500 µg/ml on day 0 were excluded from the experiment, and 10 calves were finally included. Glucose and vitamin A levels on day 7 were significantly higher (P<0.05) in the supplement group than in the control group. No significant differences were observed in haptoglobin or serum amyloid A between the groups. The number of Escherichia coli in feces was lower in the control group than in the supplement group on day 21 (P=0.06). No difference was observed in the number of bifidobacteria, but that of lactic acid bacteria was significantly higher (P<0.05) in the supplement group on day 21. The number of medications administered was significantly lower (P<0.05) in the supplement group (5.2±3.9) than in the control group (10.6±5.9) during the experimental period. The results indicated that HK-LS HS-1 is potentially beneficial for improving intestinal microbes and reducing the number of medical treatments.

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“…Flow cytometry analysis was used to evaluate the PBMC subpopulations, as described previously [20]. Briefly, the PBMCs were labeled with a primary monoclonal antibody (CD3 [MM1A], CD4 [CACT138A], CD8 [BAT82A], CD14 [CAM36A], WC1 [ILA29], or CD21 [BAQ15A]; Washington State Monoclonal Antibody Center, Pullman, WA, U.S.A.).…”

Section: Methodsmentioning

confidence: 99%

Effects of ruminal pH on gene expression in the rumen epithelium, peripheral blood mononuclear cell subpopulations, and blood metabolites from Holstein calves during weaning transition

Kim

Toji

Kizaki

et al. 2019

The Journal of Veterinary Medical Science

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We investigated the relationships between ruminal pH, gene expression in the rumen epithelium (RE), peripheral blood mononuclear cell subpopulations, and blood metabolites in Holstein calves during weaning transition. Calves (Weaning group, n=7) were assigned to one of two groups, and fed calf starter with forage (Forage group, n=3) or without forage (Starter group, n=4). Ruminal pH was measured continuously. Samples were collected at −1, 0, 1, and 3 weeks (blood and rumen fluid) or 3 weeks (rumen epithelium) after weaning. In the Weaning group, ruminal pH increased, and several blood metabolites increased (blood urea nitrogen [BUN], beta-hydroxybutyrate [BHB], and gamma-glutamyl transferase [GGT]) or decreased (total cholesterol [T-CHO] and phospholipid) after weaning. Ruminal pH was positively correlated with CD8 + CD45R − cell populations and blood metabolites (BUN, glucose, and BHB) and negatively correlated with GGT activity. The 24 hr mean ruminal pH was higher in the Forage group during weaning transition, and toll-like receptor 4 mediated signaling pathway was activated in the Starter group at 3 weeks post-weaning. The number of CD8 + CD45R − cells tended to be higher, and several blood metabolites (glucose, triglycerides, T-CHO, and phospholipid) were higher in the Forage group after weaning. Calves with higher ruminal pH also showed a greater energy metabolism status simultaneously with lesser hepatic disturbance enzymes in the peripheral blood. The results of our study indicate that serum GGT activity may be a plausible biomarker for predicting ruminal acidosis in Holstein calves during weaning transition.

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Effects of a Bacteria-Based Probiotic on Subpopulations of Peripheral Leukocytes and Their Cytokine mRNA Expression in Calves

Cited by 19 publications

References 35 publications

Pathophysiological evaluation of subacute ruminal acidosis (SARA) by continuous ruminal pH monitoring

Pathophysiological evaluation of subacute ruminal acidosis (SARA) by continuous ruminal pH monitoring

Effects of dietary feed supplementation of heat-treated <i>Lactobacillus sakei</i> HS-1 on the health status, blood parameters, and fecal microbes of Japanese Black calves

Effects of ruminal pH on gene expression in the rumen epithelium, peripheral blood mononuclear cell subpopulations, and blood metabolites from Holstein calves during weaning transition

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