Effects of 50 Hz magnetic fields on fMLP‐induced shape changes in invertebrate immunocytes: The role of calcium ion channels

Gobba, Fabriziomaria; Malagoli, Davide; Ottaviani, Enzo

doi:10.1002/bem.10102

Cited by 23 publications

(10 citation statements)

References 24 publications

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“…common stress response to exposure to static MFs, irrespective of cell type and species. Indeed, cell and surface modifications in response to exposure seems to be a highly conserved process, having been detected in invertebrate organisms as well [Ottaviani et al, 2002;Gobba et al, 2003]. However, we demonstrated that the extent of the modifications, within certain limits, is also determined by the time of exposure [Rosen, 1993;Chionna et al, 2003].…”

Section: Discussionmentioning

confidence: 52%

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Chionna¹,

Tenuzzo²,

Panzarini³

et al. 2005

Bioelectromagnetics

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Morphological modifications, i.e., cell shape, cell surface sugar residues, cytoskeleton, and apoptosis of Hep G2 cells during 24 h exposure to 6 mT static magnetic field (static MF) were studied by means of light and electron microscopy and cytochemistry. Progressive modifications of cell shape and surface were observed during the entire period of exposure to static MF. Control cells were polyhedric with short microvilli covering the cell surface, while those exposed to static MF, were elongated with many irregular microvilli randomly distributed on the cell surface. At the end of the exposure period, the cells had a less flat shape due to partial detachment from the culture dishes. However, throughout the period of exposure under investigation, the morphology of the organelles remained unmodified and cell proliferation was only partially affected. In parallel with cell shape changes, the microfilaments and microtubules, as well as the quantity and distribution of surface ConA-FITC and Ricinus communnis-FITC labeling sites, were modified in a time dependent manner. Apoptosis, which was almost negligible at the beginning of experiment, increased to about 20% after 24 h of continuous exposure. The induction of apoptosis was likely due to the increment of [Ca2+]i during exposure. In conclusion, the data reported in the present work indicates that 6 mT static MF exposure exerts time dependent biological effects on Hep G2 cells.

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Section: Discussionmentioning

confidence: 52%

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Chionna¹,

Tenuzzo²,

Panzarini³

et al. 2005

Bioelectromagnetics

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“…Moreover, previous experiments [Ottaviani et al, 2002;Gobba et al, 2003] reported an influence of 50 Hz MF, in the same range of intensity used in this study, onto Ca 2þ and K þ ion channels. This may suggest that Ca 2þ and K þ channels might be the primary target for the MF action, and this may lead to the activation of Na þ /K þ ATPase in order to restore ion homeostasis, with consequent ATP consumption, but this hypothesis requires further investigation.…”

Section: Discussionmentioning

confidence: 94%

Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC)

Bernardini

Zannoni

Turba

et al. 2006

Bioelectromagnetics

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The aim of the present study was to investigate the influence of 50 Hz sinusoidal magnetic field on Hsp27, Hsp70, and Hsp90 expression in a model of primary culture of porcine aortic endothelial cells (PAEC). We took into consideration the Hsp profile in terms of mRNA expression, protein expression and protein localization inside the cells. The choice of the cell system was motivated by the involvement of the endothelial cells in the onset of many diseases; moreover, only few reports describe the effects of extremely low frequency magnetic fields (ELF-MFs) on such cells. ELF-MF exposure induced an increase in the mRNA levels of the three proteins, which was statistically significant for Hsp70. On the contrary, we did not observe any influence on Hsp27, Hsp70, and Hsp90 protein levels. Analysis in situ by immunofluorescence revealed that ELF-MF exposure affected the cellular distribution of Hsp27; in particular a partial relocalization in the nucleus was observed.

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“…Indeed, cell and surface modifications in response to MF exposure seem to be a highly conserved process, having also been detected in invertebrate organisms (Ottaviani et al, 2002;Gobba et al, 2003).…”

Section: Plasma Membrane Cell Shape and Cytoskeletonmentioning

confidence: 98%

Bioeffects of moderate-intensity static magnetic fields on cell cultures

Dini

Abbro

2005

Micron

254

148

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Effects of 50 Hz magnetic fields on fMLP‐induced shape changes in invertebrate immunocytes: The role of calcium ion channels

Cited by 23 publications

References 24 publications

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields

Effects of 50 Hz sinusoidal magnetic fields on Hsp27, Hsp70, Hsp90 expression in porcine aortic endothelial cells (PAEC)

Bioeffects of moderate-intensity static magnetic fields on cell cultures

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