Effects of 4% icodextrin on experimental spinal epidural fibrosis

Karancı, Türker; Kelten, Bilal; Karaoğlan, Alper; Çınar, Nilgün; Mıdı, Ahmet; Antar, Veysel; Akdemir, Hidayet; Kara, Zeynep Mine Yalcinkaya

doi:10.5137/1019-5149.jtn.15079-15.1

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…Нині не існує абсолютно ефективної методики запобігання розвитку епідурального фіброзу після операції на поперековому диску, незважаючи на велику кількість експериментальних та клінічних досліджень [6][7][8]. Запропоновано методи забезпечення бар'єра між оголеними твердою оболонкою та навколишніми тканинами, однак жодний з них не дає успішного та/або тривалого клінічного ефекту [9][10][11][12].…”

Section: ключевые слова: пульпозное ядро; обогащенный тромбоцитами фиunclassified

Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats

Pedachenko¹,

Vasylieva²,

Khyzhnyak³

et al. 2020

Ukr Neurosurg J

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Section: ключевые слова: пульпозное ядро; обогащенный тромбоцитами фиunclassified

Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats

Pedachenko¹,

Vasylieva²,

Khyzhnyak³

et al. 2020

Ukr Neurosurg J

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“…Despite appropriate decompression, however, the outcomes of such spinal surgeries are not necessarily correlated with the clinical outcomes. This may be because of the development of epidural brosis and adhesions, which is considered to be a normal reaction during healing following a spinal surgery [5]. It is well accepted that epidural brosis causes pulling, stretching or compression of the associated nerve root or dura mater and can lead to persistent back and leg pain following lumbar laminectomy, known as the post laminectomy syndrome, failed back surgery syndrome (FBSS) or post spinal surgery syndrome [6,7].…”

Section: Introductionmentioning

confidence: 99%

Analysis of pain markers and epidural fibrosis caused by repeated spinal surgery in Sprague–Dawley rats

Quan

Kim

Hwang

et al. 2021

Preprint

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Background: Epidural fibrosis is one of the aetiologies of pain following a spinal revision surgery. It is reported that the specific members of the mitogen – activated protein kinases (MAPK) family might mediate neuropathic pain. However, roles of epidural fibrosis caused by repeated spinal surgeries and pain-related proteins in causing the post spinal surgery syndrome remain unknown. Using a rat spinal surgery epidural fibrosis and adhesion model, in this study, we evaluated and investigated the relationship between pain markers and epidural fibrosis. Methods: Sprague–Dawley rats that underwent the spinal surgery were divided into three groups: group A (single laminectomy), group B (two repeated surgeries) and group C (three repeated surgeries). Dural thickness was measured in each experimental group, and immunohistochemical analysis and western blotting of mitogen-activated protein kinases were performed (ERK, p38 and JNK) using the spine cord. Results: Dural thickness was 6.363 ± 1.911 µm in group A, 13.238 ± 2.123 µm in group B and 19.4 ± 2.115 µm in group C, respectively. In the western blotting, phosphorylated ERK expression gradually increased with the number of repeated surgeries, and expression in groups B (1.77-fold) and C (2.42-fold) increased as compared to expression in group A. Phosphorylated p38 showed an increasing trend with the number of repeated surgeries, and groups B (1.17-fold) and C (1.33-fold) expression increased compared with group A. However, phosphorylated JNK expression did not gradually increase with the number of repeated surgeries, and groups B (1.62-fold) and C (1.43-fold) expression increased compared with group A. Excluding phosphorylated JNK, immunohistochemical analysis revealed that phosphorylated ERK and p38 expression gradually increased with the number of repeated surgeries in the spine dorsal horn, as evidenced by western blotting. Conclusions: Repeated spinal surgeries may increase dural thickness and expression of phosphorylated ERK and p38 in the spinal dorsal horn, and it suggests that the neuropathic pain is likely induced by epidural fibrosis and that the pain increases with the number of repeated surgeries.

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“…However, occasionally, despite appropriate decompression, the outcomes of such spinal surgeries are not necessarily correlated with the clinical outcomes. This may be because of the development of epidural brosis and adhesions, which is a normal reaction during healing following spinal surgery [5]. However, epidural brosis causes pulling, stretching or compression of the associated nerve root or dura mater and can lead to persistent back and leg pain; this is known as the post laminectomy syndrome, failed back syndrome or post spinal surgery syndrome [6,7].…”

Section: Introductionmentioning

confidence: 99%

Pain Markers and Epidural Fibrosis Caused by Repeated Spinal Surgery in Sprague–Dawley Rats

Quan¹,

Kim²,

Hwang³

et al. 2020

Preprint

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Background Epidural fibrosis is one of the aetiologies of pain following spinal revision surgery. However, roles of epidural fibrosis caused by repeated spinal surgery and pain-related proteins in causing the post spinal surgery syndrome remain unknown. In this study, using a rat spinal surgery epidural fibrosis and adhesion model, we evaluate and investigated the relationship between pain marker and epidural fibrosis caused by repeated spinal surgery in Sprague-Dawley rats. Methods Sprague–Dawley rats that underwent repeated spinal surgery were divided into three groups: group A (single laminectomy), group B (two repeated surgeries) and group C (three repeated surgeries). Dural thickness was measured in each experimental group, and immunohistochemical analysis and western blotting of mitogen-activated protein kinases were performed (ERK, p38 and JNK). Results Dural thickness was 6.363 ± 1.911 µm in group A, 13.238 ± 2.123 µm in group B and 19.4 ± 2.115 µm in group C. In western blotting, phosphorylated ERK expression was higher in groups B (1.77 fold) and C (2.42 fold) than in group A. Phosphorylated p38 expression was higher in groups B (1.17 fold) and C (1.33 fold) than in group A. Immunohistochemical analysis revealed that phosphorylated ERK and p38 expression gradually increased with the number of repeated surgeries, as evidenced by western blotting. Conclusions Repeated spinal surgery may increase dural thickness and expression of phosphorylated ERK and p38 in the spinal dorsal horn, suggesting that pain increases with repeated surgery.

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Effects of 4% icodextrin on experimental spinal epidural fibrosis

Cited by 4 publications

References 18 publications

Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats

Effect of platelet-rich fibrin matrix in complex with artificial material Nubiplant on expression of chondrogenic marker genes and morphogenesis of the nucleus pulposus cells of intervertebral discs in rats

Analysis of pain markers and epidural fibrosis caused by repeated spinal surgery in Sprague–Dawley rats

Pain Markers and Epidural Fibrosis Caused by Repeated Spinal Surgery in Sprague–Dawley Rats

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