Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA

Cavallin, Jenna E.; Beihoffer, Jon; Blackwell, Brett R.; Cole, Alexander R.; Ekman, Drew R.; Hofer, Rachel N.; Jastrow, Aaron; Kinsey, Julie; Keteles, Kristen; Maloney, Erin M.; Parman, J.; Winkelman, Dana L.; Villeneuve, Daniel L.

doi:10.1016/j.envpol.2021.117928

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…As a result, effect-based methods, which include in vitro assays to detect biological activity (bioassays), are recommended for contamination monitoring as they can detect the cumulative response of active chemicals in complex matrices. − Batteries of in vitro bioassays covering different cellular toxicity pathways have been applied to drinking water, surface water, reclaimed water, and wastewater. − Studies from the past decade have consistently shown that urban waters contain chemicals that can activate assays indicative of xenobiotic metabolism, endocrine disruption, adaptive stress responses, and cell growth. − Advanced wastewater treatment plants are usually able to attenuate the majority of these responses by reducing concentrations of chemicals causing these effects, and no or low biological activity has been reported in highly treated water. , However, the bioassays deployed were “those that were available” reviewed elsewhere, and concerns remain that effects outside of the scope covered by these assays may remain undiscovered (i.e., “unknown unknowns”).…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes

Rogers,

Leusch,

Chambers

et al. 2024

Environ. Sci. Technol.

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The presence of numerous chemical contaminants from industrial, agricultural, and pharmaceutical sources in water supplies poses a potential risk to human and ecological health. Current chemical analyses suffer from limitations, including chemical coverage and high cost, and broad-coverage in vitro assays such as transcriptomics may further improve water quality monitoring by assessing a large range of possible effects. Here, we used high-throughput transcriptomics to assess the activity induced by field-derived water extracts in MCF7 breast carcinoma cells. Wastewater and surface water extracts induced the largest changes in expression among cell proliferation-related genes and neurological, estrogenic, and antibiotic pathways, whereas drinking and reclaimed water extracts that underwent advanced treatment showed substantially reduced bioactivity on both gene and pathway levels. Importantly, reclaimed water extracts induced fewer changes in gene expression than laboratory blanks, which reinforces previous conclusions based on targeted assays and improves confidence in bioassay-based monitoring of water quality.

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Section: Introductionmentioning

confidence: 99%

High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes

Rogers,

Leusch,

Chambers

et al. 2024

Environ. Sci. Technol.

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“…The primary applications of EBMs include the assessment of treatment efficacy of engineered or natural processes (see Bain et al, 2014; Sossalla et al, 2021), evaluation of time trends in natural and engineered systems (see Cavallin et al, 2021), and benchmarking the quality of water from different origins (see Escher et al, 2014; Leusch, Neale, et al, 2018). Therefore, the effects are typically compared within a process, along a time axis, or across different locations.…”

Section: Introductionmentioning

confidence: 99%

Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning

Neale

Escher

Baat

et al. 2023

Enviro Toxic and Chemistry

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“…Furthermore, NAMs can be used to generate a wealth of data that can supplement experimental output, providing a more holistic view of the ecotoxicological risk potential of chemical(s) of concern. As such, the incorporation of NAM‐based techniques into ecotoxicological analyses and environmental risk assessments has recently been an active area of emphasis among governmental, academic, and industrial organizations throughout North America (Ankley et al, 2021; Blackwell et al, 2017; Cavallin et al, 2021; Corsi et al, 2019; Ekman et al, 2013; Li et al, 2017) and Europe (Brunner et al, 2019; Busch et al, 2016; Louisse et al, 2018; Schollée et al, 2015). Collectively, these efforts have highlighted many strategies and computational techniques that can be used to identify chemicals of potential ecotoxicological concern within aquatic and terrestrial environments.…”

Section: Introductionmentioning

confidence: 99%

A framework for prioritizing contaminants in retrospective ecological assessments: Application in the Milwaukee Estuary (Milwaukee, WI)

Maloney

Villeneuve

Blackwell

et al. 2023

Integr Envir Assess & Manag

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Watersheds are subjected to diverse anthropogenic inputs, exposing aquatic biota to a wide range of chemicals. Detection of multiple, different chemicals can challenge natural resource managers who often have to determine where to allocate potentially limited resources. Here, we describe a weight‐of‐evidence framework for retrospectively prioritizing aquatic contaminants. To demonstrate framework utility, we used data from 96‐h caged fish studies to prioritize chemicals detected in the Milwaukee Estuary (WI, USA; 2017–2018). Across study years, 77/178 targeted chemicals were detected. Chemicals were assigned prioritization scores based on spatial and temporal detection frequency, environmental distribution, environmental fate, ecotoxicological potential, and effect prediction. Chemicals were sorted into priority bins based on the intersection of prioritization score and data availability. Data‐limited chemicals represented those that did not have sufficient data to adequately evaluate ecotoxicological potential or environmental fate. Seven compounds (fluoranthene, benzo[a]pyrene, pyrene, atrazine, metolachlor, phenanthrene, and DEET) were identified as high or medium priority and data sufficient and flagged as candidates for further effects‐based monitoring studies. Twenty‐one compounds were identified as high or medium priority and data limited and flagged as candidates for further ecotoxicological research. Fifteen chemicals were flagged as the lowest priority in the watershed. One of these chemicals (2‐methylnaphthalene) displayed no data limitations and was flagged as a definitively low‐priority chemical. The remaining chemicals displayed some data limitations and were considered lower‐priority compounds (contingent on further ecotoxicological and environmental fate assessments). The remaining 34 compounds were flagged as low or medium priority. Altogether, this prioritization provided a screening‐level (non‐definitive) assessment that could be used to focus further resource management and risk assessment activities in the Milwaukee Estuary. Furthermore, by providing detailed methodology and a practical example with real experimental data, we demonstrated that the proposed framework represents a transparent and adaptable approach for prioritizing contaminants in freshwater environments. Integr Environ Assess Manag 2023;00:1–21. © 2022 SETAC

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Effects-based monitoring of bioactive compounds associated with municipal wastewater treatment plant effluent discharge to the South Platte River, Colorado, USA

Cited by 9 publications

References 40 publications

High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes

High-Throughput Transcriptomics of Water Extracts Detects Reductions in Biological Activity with Water Treatment Processes

Effect‐Based Trigger Values Are Essential for the Uptake of Effect‐Based Methods in Water Safety Planning

A framework for prioritizing contaminants in retrospective ecological assessments: Application in the Milwaukee Estuary (Milwaukee, WI)

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