Effects and applications of arthropod steroid hormones (ecdysteroids) in mammals

Dinan, Laurence; Lafont, René

doi:10.1677/joe.1.06900

Cited by 173 publications

(173 citation statements)

References 57 publications

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“…Indeed, much as seen with Nos up-regulation, ecdysone supplementation in vertebrates results in decreased appetite, cholesterol synthesis, and weight gain, while at the same time increasing muscle mass and endurance (Lafont and Dinan 2003;Dinan and Lafont 2006;Kizelsztein et al 2009). Given these apparent abilities to switch fat cell activity from lipid storage to lipid mobilization, a better understanding of the signals and mechanisms underlying NO and ecdysone-mediated effects should provide new insights and possible uses in metabolism based disorders.…”

Section: Nonautonomous Actions On Lipid Metabolismmentioning

confidence: 99%

Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75

Cáceres¹,

Necakov²,

Schwartz³

et al. 2011

Genes Dev.

121

173

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Nitric oxide gas acts as a short-range signaling molecule in a vast array of important physiological processes, many of which include major changes in gene expression. How these genomic responses are induced, however, is poorly understood. Here, using genetic and chemical manipulations, we show that nitric oxide is produced in the Drosophila prothoracic gland, where it acts via the nuclear receptor ecdysone-induced protein 75 (E75), reversing its ability to interfere with its heterodimer partner, Drosophila hormone receptor 3 (DHR3). Manipulation of these interactions leads to gross alterations in feeding behavior, fat deposition, and developmental timing. These neuroendocrine interactions and consequences appear to be conserved in vertebrates.[Keywords: E75; DHR3; nitric oxide; Drosophila; metamorphosis; ecdysone; metabolism] Supplemental material is available for this article. In previous work, we showed that ecdysone-induced protein 75 (E75, also known as Eip75B; NR1D3) (Tweedie et al. 2009) contains heme constitutively bound to its ligand-binding domain (LBD), and that amino acids coordinately bound to the heme iron can be displaced in vitro by changes in redox state or the presence of nitric oxide (NO) gas (Pardee et al. 2004;Reinking et al. 2005;Marvin et al. 2009). In turn, these structural changes negate the ability of E75 to repress transcription and to reverse the positive transcriptional activity of its heterodimer partner, Drosophila hormone receptor 3 (DHR3; also known as DHR46, NR1F4) (Tweedie et al. 2009). Here, we look to see whether these interactions are relevant in vivo and, if so, what their roles are.One of the best-characterized roles of E75 and DHR3 in vivo is within the nuclear receptor (NR) transcriptional hierarchy that controls, and responds to, the production of the metamorphosis-inducing hormone ecdysone (diagrammed in Supplemental Fig. 1A). Upon binding ecdysone, the ecdysone receptor (EcR) acts as a heterodimer with a second NR, ultraspiracle (USP), to activate transcription of DHR3 and the E75 isoform E75A (Koelle et al. 1991;Lam et al. 1997Lam et al. , 1999White et al. 1997;Bialecki et al. 2002). DHR3 then promotes its own continued expression as well as that of the E75 splice variant E75B and the downstream NR gene bFtz-F1. bFTZ-F1, in turn, activates the expression of ecdysone synthetic enzyme genes (Lavorgna et al. 1993;Woodard et al. 1994;Broadus et al. 1999), resulting in another round of ecdysone production. The major site of larval ecdysone production is the prothoracic gland (PG) (diagrammed in Supplemental Fig. 1B). The PG is also a major site of NO synthase (Nos) expression (Wildemann and Bicker 1999). Hence, we looked to see whether E75 and NO are present and interactive in this tissue, with the hypothesis that activation of bFtz-F1 transcription by DHR3 requires inactivation of E75 isoforms by NO. As predicted, all of the above-listed genes are expressed in the PG toward the end of third instar development, and disruption of their expression or activity leads to molec...

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Section: Nonautonomous Actions On Lipid Metabolismmentioning

confidence: 99%

Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75

Cáceres¹,

Necakov²,

Schwartz³

et al. 2011

Genes Dev.

121

173

View full text Add to dashboard Cite

show abstract

“…Specialists of ecdysteroid research declared an interesting spectrum of effects of ecdysteroids on mammalian organisms, such as, for example, the ability of these compounds to improve protein incorporation. Báthori [2], Dinan and Lafont [3], and Dinan [4] published indepth reviews on the effects and applications of ecdysteroids. Lafont et al [5] summarized the excretion and metabolism of ecdysone injected into white mice.…”

Section: Introductionmentioning

confidence: 99%

HPLC analysis and blood-brain penetration of 20-hydroxyecdysone diacetonide

Kalász

Hunyadi

Tekes

et al. 2017

Acta Chromatographica

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Summary. Blood-brain penetration of 20-hydroxyecdysone 2,3;20,22-diacetonide (20DA) has been scouted using chromatographic methods. In vivo experiments were performed by treating male Wistar rats intraperitoneally (i.p.) with a dose of 50 mg kg −1 20DA. Control experiment was done by using 20-hydroxyecdysone (20E). Definite brain penetration of 20DA was found by using high-performance liquid chromatography (HPLC), while 20E does not show this type of distribution.

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“…The major phytoecdysteroid present in most plant ecdysteroids-containing plants is 20E, which is also the major ecdysteroid in insects Lafont 1997). If the effects and application of 20E in animals are extensively studied (Lafont and Dinan, 2003;Dinan and Lafont (2006), its effects and application on plants is rarely studied and the importance of 20E in the life cycle of plants has not been elucidated. However, there are several evidences that 20E has not a hormonal role within the plants (Lafont 1998;Dinan 2001).…”

Section: Discussionmentioning

confidence: 99%

Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth

Bakrim¹,

Lamhamdi²,

Sayah³

et al. 2007

Afr. J. Biotechnol.

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20-hydroxyecdysone (20E)is the major phytoecdysteroid of about 6% of plants. Its role in plant physiology has not been fully elucidated. In this work we studied the effects of 20E application on some morphological and biochemical parameters of tomato, Lycopersicum esculentum, seed during germination and seedling development (5 d). We compared the 20E effects with the action of phytohormones: gibberellic acid (GA 3 ), naftalen acetic acid (NAA), benzyl amino purine (BAP). NAA treatment resulted in marked reduction in shoot length. GA 3 treatment promoted maximal shoot elongation. BAP affected negatively shoot length only at late stages, while 20E application stimulated shoot elongation at early stages and reduced it on the fifth day; NAA inhibited root elongation all along the test period. GA 3 treatment had no effect on root length, whereas BAP showed strong inhibition on root elongation. On the other hand, 20E showed a weak inhibition of root elongation on the fifth day. As compared to control and to other treatments, NAA and 20E provoked a drastic decrease in protein contents during seedling growth, whereas a high increase was observed under BAP treatment. Electrophoresis revealed that protein bands were not degraded and mobilized after NAA treatment while in control or after 20E and others phytohormones applications, protein patters displayed weak band intensities and some of them were not detected. NAA, GA 3 and BAP provoked a decrease in proline content during seedlings, while the effect of 20E on proline levels varied during germination and plantlet development. This work showed that 20E like phytohormones fulfil some bioactive actions during germination and seedlings growth in tomato.

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Effects and applications of arthropod steroid hormones (ecdysteroids) in mammals

Cited by 173 publications

References 57 publications

Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75

Nitric oxide coordinates metabolism, growth, and development via the nuclear receptor E75

HPLC analysis and blood-brain penetration of 20-hydroxyecdysone diacetonide

Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth

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