Effects of plant hormones and 20-hydroxyecdysone on tomato (Lycopersicum esculentum) seed germination and seedlings growth

Abstract

20-hydroxyecdysone (20E)is the major phytoecdysteroid of about 6% of plants. Its role in plant physiology has not been fully elucidated. In this work we studied the effects of 20E application on some morphological and biochemical parameters of tomato, Lycopersicum esculentum, seed during germination and seedling development (5 d). We compared the 20E effects with the action of phytohormones: gibberellic acid (GA 3 ), naftalen acetic acid (NAA), benzyl amino purine (BAP). NAA treatment resulted in marked reduction in shoot length. GA 3 treatment promoted maximal shoot elongation. BAP affected negatively shoot length only at late stages, while 20E application stimulated shoot elongation at early stages and reduced it on the fifth day; NAA inhibited root elongation all along the test period. GA 3 treatment had no effect on root length, whereas BAP showed strong inhibition on root elongation. On the other hand, 20E showed a weak inhibition of root elongation on the fifth day. As compared to control and to other treatments, NAA and 20E provoked a drastic decrease in protein contents during seedling growth, whereas a high increase was observed under BAP treatment. Electrophoresis revealed that protein bands were not degraded and mobilized after NAA treatment while in control or after 20E and others phytohormones applications, protein patters displayed weak band intensities and some of them were not detected. NAA, GA 3 and BAP provoked a decrease in proline content during seedlings, while the effect of 20E on proline levels varied during germination and plantlet development. This work showed that 20E like phytohormones fulfil some bioactive actions during germination and seedlings growth in tomato.

Effect of seed priming with plant growth regulators and temperature on Withania somnifera L. DUNAL

“…By providing long soaking period of growth regulators we can improve the germination percentage. IAA and IBA induce c ellular elongation, c ell division and emergence of radical across the endosperm and seed coat [2]. IAA and IBA also give a better result as far as the seed germination is concern in both the varieties but they were not performing well as GA 3.…”

Identificación de auxinas, giberelinas y citocininas en frutos de frambuesa (Rubus idaeus)

“…La presencia de giberelinas A4 y A7 en el fruto podría reflejar la importancia de estas hormonas en el proceso de crecimiento como lo señalan varios autores (Alghonmeen et al 2020, Tombegavani et al 2020. Está bien establecido que cuando se aplican GA4 y GA7 exógenas ambas hormonas estimulan el crecimiento del fruto en tomate, pimiento y otros cultivos (Bakrim et al 2007y Ramírez et al 2016. Las giberelinas A4 y A7 son hormonas endógenas que se desplazan por diferentes órganos de la planta; mientras que las giberelinas A44 y A53 se clasifican como hormonas biológicamente inactivas (Ramírez et al 2018, Alghonmeen et al 2020, Tombegavani et al 2020.…”

Nanobiotechnological approach to stimulate the growth of Astragalus chrysochlorus (Leguminosae) using In-Silico and In-Vivo strategies.

“…Speci c endogenic hormones and their concentrations play an important role in seed dormancy, growth, and seedlings (McCourt 1999; Kucera et al, 2005). Relations between endogenous hormone concentrations and speci c developmental periods, effects of administered auxins, and metabolic activity suggest that hormones are involved in these metabolic processes (Bakrim et al, 2007).…”