Effector cell signature in peripheral blood following nasal allergen challenge in grass pollen allergic individuals

Shamji, Mohamed H.; Bellido, V.; Scadding, Guy W.; Layhadi, Janice A.; Cheung, Delica K. M.; Calderón, Moisés A.; Asare, Adam; Zhong, Gongxun; Turka, Laurence A.; Tchao, Nadia K.; Togias, Alkis; Phippard, Deborah; Durham, Stephen R.

doi:10.1111/all.12543

Cited by 31 publications

(25 citation statements)

References 33 publications

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“…This study described changes in peripheral blood cells, although allergen challenge was induced only once and may not have accurately reflected the changes caused by natural exposure during allergy season [44]. In patients with allergic rhinitis, the response to provocational exposure may be different from the response during pollen season [32].…”

Section: Discussionmentioning

confidence: 99%

Th17 responses are not altered by natural exposure to seasonal allergens in pollen-sensitive patients

Schramm

Jasiewicz-Honkisz

Osmenda

et al. 2016

Allergy Asthma Clin Immunol

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BackgroundAllergic rhinitis affects 10–30 % of the global population and this number is likely to increase in the forthcoming years. Moreover, it commonly co-exists with allergic asthma as a chronic allergic respiratory syndrome. While the involvement of Th2 cells in allergy is well understood, alterations of pro-inflammatory Th17 responses remain poorly characterized. The aim of our study was to determine whether natural seasonal allergen exposure causes changes in T cell subset characteristics in patients with allergic rhinitis and asthma.MethodsSixteen patients with allergic rhinitis/atopic asthma (9M, 7F; age 31.8 ± 12.1) and 16 healthy controls were recruited into the study (9M, 7F; age 31.2 ± 5.3). Blood samples were collected from the patients 1–3 months before pollen season (visit 1), within 7 days of the appearance of pollen/initiation of allergic symptoms (visit 2) and 2 weeks after visit 2 following the introduction of symptomatic treatment with antihistamines (visit 3). Flow cytometry was used to assess major T cell subsets (naïve, central memory, effector memory and CD45RA+ effector) and key T cell cytokine production (IFNγ, IL-17A, TNF and IL-4) using intracellular staining. Data were analyzed using repeated measures ANOVA and paired t test.ResultsAs expected, an increase in the percentage of IL‐4+ CD4+ cells was observed during natural pollen exposure in patients with allergic respiratory syndrome. No significant changes were observed in the production of other cytokines, including Th17 cells, which tended to be lower than in the control population but unchanged during pollen exposure. Introduction of antihistamine treatment led to only moderate changes in cytokine production from CD4 and CD8 T cells. Selective changes in CD8+ T cells were observed during natural pollen exposure including a decrease in transient cells (with features of CD45RA+ and CD45RO+ cells) and a decrease in the percentage of central memory cells in the peripheral circulation. Within the CD4 cell group the total percentage of CD45RA positive CD4 cells was increased during pollen exposure.ConclusionsTh1 and Th17 responses are not altered during pollen season but allergen exposure affects T cell activation and memory cell status in patients with allergic respiratory syndrome.Electronic supplementary materialThe online version of this article (doi:10.1186/s13223-016-0157-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Th17 responses are not altered by natural exposure to seasonal allergens in pollen-sensitive patients

Schramm

Jasiewicz-Honkisz

Osmenda

et al. 2016

Allergy Asthma Clin Immunol

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“…58 Dupilumab Anti-IL4Rα Subcutaneous 106 activation of DC, basophils and T cells in the peripheral blood following NAC. 60 DCs from patients with AR and asthma display impaired IL-12 and IFN-α production in response to toll-like receptor (TLR) 4 and TLR9 activation, respectively, as well as impaired induction of IL-10-producing CD4 + T cells. 61…”

Section: Environmental Factorsmentioning

confidence: 99%

Recent developments and highlights in allergic rhinitis

Meng

Wang

Zhang

2019

Allergy

124

105

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Allergic rhinitis (AR) is a disease with high prevalence all over the world and therefore needs to be thoroughly investigated and treated accordingly. The mechanisms underlying the pathology and treatment of AR have been widely studied, but many aspects remain unclear and warrant further investigations. This review presents an overview of recently published papers highlighting the risk factors, mechanisms, and treatment of AR. Additionally, recent studies discussing the role of single nucleotide polymorphism, DNA methylation, regulatory B cells, group 2 innate lymphoid cells, immunotherapy, and biologics in AR are also covered. K E Y W O R D Sallergic rhinitis, biologics, epigenetics, risk factors, treatment | 2321 MENG Et al.

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“…In the ED study, which included predominantly venom‐allergic adult patients, there was a substantial reduction (80%) in circulating basophils during anaphylactic reactions, and these findings were replicated in peanut‐allergic individuals experiencing allergic reactions during double‐blind placebo‐controlled peanut challenge. In contrast to previous studies which monitored basophils at sites of allergen challenge, this study assessed basophils in the peripheral blood, including absolute basophil count measured by flow cytometry using microbeads, with basophils identified as CD123 + HLA‐DR ‐ or CRTh2 + CD303 ‐ CD123 + cells . Basophil migration was confirmed using whole blood gene expression analysis of genes which are specific for basophils, including the α‐subunit of the high‐affinity IgE receptor ( FCER1A ) , carboxypeptidase A3 ( CPA3 ) and histidine decarboxylase ( HDC ) .…”

Section: Basophil Migrationmentioning

confidence: 99%

“…In contrast to previous studies which monitored basophils at sites of allergen challenge, 31,37,51,79 this study 50 assessed basophils in the peripheral blood, including absolute basophil count measured by flow cytometry using microbeads, with basophils identified as CD123 + HLA-DR -80 or CRTh2 + CD303 -CD123 + cells. 81 Basophil migration was confirmed using whole blood gene expression analysis of genes which are specific for basophils, including the a-subunit of the high-affinity IgE receptor (FCER1A), carboxypeptidase A3 (CPA3) and histidine decarboxylase (HDC). 50 FceRI is expressed on mast cells and basophils as tetramers (abc 2 ) as well as on antigen-presenting cells, although at substantially lower levels and only as trimmers (ac 2 ).…”

Section: Migration During Anaphylaxismentioning

confidence: 99%

Important and specific role for basophils in acute allergic reactions

Korošec

Gibbs

Rijavec

et al. 2018

Clin Experimental Allergy

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SummaryIgE‐mediated allergic reactions involve the activation of effector cells, predominantly through the high‐affinity IgE receptor (FcεRI) on mast cells and basophils. Although the mast cell is considered the major effector cell during acute allergic reactions, more recent studies indicate a potentially important and specific role for basophils and their migration which occurs rapidly upon allergen challenge in humans undergoing anaphylaxis. We review the evidence for a role of basophils in contributing to clinical symptoms of anaphylaxis and discuss the possibility that basophil trafficking during anaphylaxis might be a pathogenic (to target organs) or protective (preventing degranulation in circulation) response. Finally, we examine the potential role of basophils in asthma exacerbations. Understanding the factors that regulate basophil trafficking and activation might lead to new diagnostic and therapeutic strategies in anaphylaxis and asthma.

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Effector cell signature in peripheral blood following nasal allergen challenge in grass pollen allergic individuals

Abstract: Basophil, T-cell, and dendritic cell activation increased the frequency of allergen-driven IL-4(+) CD4(+) T cells, and T-cell proliferative responses are detectable in the periphery after NAC. These data confirm systemic cellular activation following a local nasal provocation.

Cited by 31 publications

References 33 publications

Th17 responses are not altered by natural exposure to seasonal allergens in pollen-sensitive patients

Th17 responses are not altered by natural exposure to seasonal allergens in pollen-sensitive patients

Recent developments and highlights in allergic rhinitis

Important and specific role for basophils in acute allergic reactions

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