Effectiviness of Lime Peel Extract (&lt;i&gt;Citrus aurantifolia&lt;/i&gt; Swingle) against C-Reactive Protein Levels in Alloxan-Induced Wistar Rats

Suryadinata, Rivan Virlando; Lorensia, Amelia; Sefania, Kezia

doi:10.29313/gmhc.v9i1.6227

Cited by 3 publications

(3 citation statements)

References 20 publications

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“…In this study, the serum level CRP was significantly high in the inflammation control group (1.70±0.07ng/ml) when compared with 7 days (1.05±0.06ng/ml) and 14 days (0.93±0.05ng/ml) Bio-Clean II treated rats, as well as the zero control rats (0.92±0.03ng/ml). The outcome of this study is consistent with the work of Suryadinata et al, who evaluated the effect of 30 days treatment with lime peel extracts (Citrus aurantifolia swingle) against C-reactive protein levels in Alloxan-induced wistar rats [40]. In their study, CRP value was reported to be high in the alloxan induced rats (>12mg/ mL) when compared to the non-induced rats (<6mg/mL) and lime peel extract treated rats (<12mg/mL).…”

Section: Discussionsupporting

confidence: 90%

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

2022

JCEI

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Background: Bio-Clean II has been previously shown to fight viral infection, boost immunity, and possesses anti-inflammatory properties by regulating the serum level of inflammatory cytokines, as well as T-Helper 4 and Cytotoxic T-Lymphocytes in rats exposed to purified bacterial lipopolysaccharide (LPS). Aim: The aim of this study is to assess the effects of Bio-Clean II on the C-reactive protein (CRP), corticosterone (CORT) and anti-phospholipid antibodies (aPLs) levels in rats exposed to bacterial lipopolysaccharide (LPS) using animal model. Materials and Methods: A total of 36 male Wistar rats weighing 150g±50g (mean±SD) were purchased and randomly assigned to six (6) groups of 6 rats each. Group 1, 2, 3, 5 and 6 were induced with a single dose of 5mg/Kg of purified LPS® (E.coli 0127:B8, Sigma-Aldrich, St. Louis, USA), administered through intraperitoneal route using 1ml sterile needle and syringe, except for group 4 which served as the zero control (given water and food only throughout the experiment). Group 1 served as the inflammation control. Group 2 which served as the positive control received 50 mg diclofenac/kg [bid] and 500 mg ciprofloxacin/kg [bid] (positive control) in place of the Bio-Clean. Group 3 which served as the negative control received sterile phosphate buffer saline (PBS). While rats in group 5 and group 6 were treated orally with the herbal remedy “Bio-Clean II” for 7 days and 14 days, respectively. After which, the rats were killed and a cardiac blood specimen was taken from each rat and transferred to plain bottles to clot. Serum was obtained from the clotted blood by centrifugation. The serum levels of C-reactive protein, corticosterone and antiphospholipid antibodies were assayed using ELISA kits, supplied by Elabscience Biotechnology Inc, USA. Data generated were subjected to Statistical Package for Social Scientists-Version 20 (SPSS-20). Results: The outcome of this results show that the serum level of C-reactive protein of the 7 days (1.05±0.06ng/ml) and 14 days (0.93±0.05ng/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.002 and p=0.000, respectively) when compared to the inflammation control group (1.70±0.07ng/ml). Similarly, the serum level of anti-phospholipid antibodies of the 7 days (6.40±0.67 Uml) and 14 days (4.27±0.66 U/ml) Bio-Clean II treated rats was found to be significantly lower (p=0.02 and p=0.008, respectively) when compared to the inflammation control group (16.47±1.53 Uml). Meanwhile, the corticosterone level of the 7 days Bio-clean treated rats (9.40±1.30ng/mL) was found to be non-significantly lower (p=0.812) in comparison to the inflammation control (13.50±2.50ng/mL); while that of the 14 days Bio-clean II treated rats (6.80±1.00ng/mL) was significantly lower (p=0.026). Conclusion: The outcom

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Section: Discussionsupporting

confidence: 90%

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

2022

JCEI

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“…italica) contains various types of non-enzymatic antioxidants and high polyphenol, including flavonoids, which is estimated to reduce free radicals and inflammation caused by cigarette smoke exposure. 33,34 This vegetable also contains selenium, and is known to potentially increase various enzymatic antioxidants, including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). 35…”

Section: Articlementioning

confidence: 99%

Brassica oleracea var. italica extract reducing free radicals and inflammation initiated by an exposure to cigarette smoke

Suryadinata

Aditya²,

Wiana³

et al. 2023

HEALTHC LOW-RESOUR S

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Introduction: Herbal extracts are often administered to cigarette smokers to prevent excessive free radicals. These include Brassica oleracea var. Italica, known to contain high antioxidant flavonoids and selenium micronutrients. Therefore, this study aims to determine the efficacy of Brassica oleracea var extract. italica in reducing the free radicals and inflammation present in experimental animals exposed to cigarette smoke. Design and Methods: This research was conducted based on an experimental method using a randomized controlled trial (RCT) for 21 days. The animals used were divided into six groups (negative control, positive control, and four treatments). Particularly, the positive control and treatment groups were exposed to cigarette smoke for 2 minutes, twice a day, at 50 PPM CO levels. The treatment groups were administered the extract at different doses (0.5 ml; 0.75 ml; 1 ml; 1.25 ml), before assessing the blood level of malondialdehyde and C-Reactive Protein. Result: The results showed the tendency for exposure to smoke to increase the number of free radicals and stimulate inflammation responses in the body (P<0,05). In addition, a strong correlation between variables was established (p=0.000; r=0.713). Conclusions: Broccoli extracts (Brassica oleracea L. var. Italica) administration has the potential to cause a decline in the two aspects, including free radicals and inflammation responses resulting from exposure to cigarette smoke.

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“…Which are obtained not only from its main part (the pulp) but also its peel. (18) Therefore, this study aimed further understand the effect of antioxidant in lime peel to decrease free radicals induced by hyperglycemia. In initial form, this study used experimental animals, i.e.…”

Section: Introductionmentioning

confidence: 99%

Reduction of free radicals in hyperglycemic conditions through the administration of lime peel extract (Citrus aurantifolia swingle)

Suryadinata

Sefania

Boengas

2022

IJND

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ABSTRAKLatar Belakang: Kondisi hiperglikemia tidak selalu terjadi pada penderita diabetes. Stress oksidatif merupakan salah satu proses pathogenesis yang ditimbulkan terhadap penyakit akibat hiperglikemia. Hal tersebut berkaitan dengan peningkatan Reactive Oxygen Species (ROS) dan penurunan aktivitas antioksidan. Pemberian asupan antioksidan dari luar tubuh diharapkan dapat membantu menetralisir radikal bebas yang berlebihan.Tujuan: Untuk mengetahui efektivitas ektrak kulit jeruk nipis terhadap pernurunan radikal bebas pada kondisi hiperglikemia. Metode: Penelitian ini merupakan eksperimental dengan post-test control group design dengan menggunakan tikus wistar di laboratorium Fakultas Kedkteran, Universitas Surabaya. Kondisi hiperglikemia pada hewan coba dilakukan melalui induksi aloksan dan selanjutnya diberikan ekstrak kulit jeruk nipis (2,35 mg; 4,7 mg; 9,4 mg) selama 30 hari dengan membagi menjadi 5 kelompok perlakukan. Parameter untuk mengetahui kadar radikal bebas pada penelitian ini adalah kadar malondialdehid.Hasil: Pada penelitian memperlihatkan bahwa pada kelompok yang diberikan ektrak kulit jeruk nipis akan mengalami penurunan kadar malondialdehid jika dibandingkan dengan kelompok lainnya (p<0,05). Hasil rerata kadar malondialdehid terendah pada dosis 9,4 mg (1,67±0,10).Kesimpulan: Pemberian ekstrak kulit jeruk nipis dapat menurunkan kadar malondialdehid pada kondisi hiperglikemia. KATA KUNCI: Citrus aurantifolia; hiperglikemia; malodialdehid; radikal bebas ABSTRACTBackground: Hyperglycemia condition does not always occur in diabetes. Oxidative stress is a process of pathogenesis diseases as a result of hyperglycemia. This is associated with increased Reactive Oxygen Species (ROS) and decreased antioxidant activity. Antioxidants intakes outside the body is expected to neutralize excessive free radicals.Objectives: To determine the effectiveness of lime peel extract against free radicals induced by hyperglycemia.Methods: This research is an experimental post-test control group design using wistar rats in the laboratory of the Faculty of Medicine, University of Surabaya. Hyperglycemia conditions in experimental animals were carried out through alloxan induction and then given lime peel extract (2,35 mg; 4,7 mg; 9,4 mg) for 30 days by dividing into 5 treatment groups. The parameter to determine the levels of free radicals in this study is the levels of malondialdehydeResults: Showed that the group given lime peel extract experienced a decrease in malondialdehyde levels when compared to the other groups (p<0.05). The lowest mean malondialdehyde level was at a dose of 9.4mg (1.67±0.10). Conclusion: The administration of lime peel extract can reduce levels of Malondialdehyde induced by hyperglycemia. KEYWORDS: Citrus aurantifolia; free radicals; hyperglycemia; malondialdehyde

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Effectiviness of Lime Peel Extract (<i>Citrus aurantifolia</i> Swingle) against C-Reactive Protein Levels in Alloxan-Induced Wistar Rats

Cited by 3 publications

References 20 publications

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

Immunomodulatory Effects of Bio-Clean II Herbal Remedy on C-reactive protein, Corticosterone and Antiphospholipid antibodies in Rats exposed to Purified Bacterial Lipopolysaccharide

<em>Brassica oleracea</em> var. italica extract reducing free radicals and inflammation initiated by an exposure to cigarette smoke

Reduction of free radicals in hyperglycemic conditions through the administration of lime peel extract (Citrus aurantifolia swingle)

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