Effectivenessof Casein Phospopeptide Amormphous Calcium Phosphate With or Without Fluoride on Reminelization of Enamel Caries- Like Lesions in Primary Teeth

Abstract

Objective: To evaluate and compare the remineralization effect of casein phospho-peptide amorphous calcium phosphate with or without fluoride "MI paste"&"MI Paste Plus" respectively" on the re-mineralization of enamel caries-like lesions in primary teeth. Material and methods:Artificial caries lesion was made on the labial surface of 40 extracted or exfoliated sound primary teeth which were then divided into 2 halves; one half was treated with one of the tested remineralizing agent "MI paste" or "MI Paste Plus"" while the other half served as a control. The lesions were evaluated both qualitatively using a polarized light microscope and quantitatively by measuring "calcium and phosphate" content using "energy-dispersive X-ray spectroscopy". Data were collected and analyzed by using paired t-test to compare the control and the study groups for each material, while the "t-test" was used to compare between the two study groups.Results: Both "MI paste" and "MI Paste Plus" showed significant re-mineralization to the subsurface enamel lesions. "MI Paste Plus" showed significantly better results than "MI paste" with an increased amount of calcium "t=5.59 p=<0.0001", Ca/phosphate ratio "t=4.08 p=0.003" and reduction in the amount of phosphate content "t=4.57 p= 0.001". Conclusion:Both "MI paste" and "MI Paste Plus" were effective in enamel remineralization, the addition of fluoride in "MI Paste Plus" acted synergistically with the casein phospho-peptide amorphous calcium phosphate and gave better remineralization effect on enamel.

Effect of (CPP-ACP) and (CPP-ACP with fluoride) on Chemical and Morphological Analysis of Bovine Teeth Following Demineralization/Remineralization Cycles” An In-vitro Study

“…The protective layer in MI Paste Plus was more evident with calcified deposits and concentrated along the porous defects when compared with MI paste. This was in accordance with many studies (21,22,23) . Both test groups showed significant remineralization after twenty-one days of treatment with "MI Paste or MI Paste Plus".…”

“…(24,25) The efficacy of their technology in preventing demineralization and boosting re-mineralization of enamel in vivo (26,27) . Our study demonstrated that addition of fluoride to the paste "MI Paste Plus", had accentuated better remineralization, this was due to the ability of "CPP" to connect fluoride as well as calcium and phosphate, forming calcium fluoride phosphate as soluble complexes (23) . These findings were supported by many studies (28,29) .…”

Color Stability of Artificial White Spot Lesions treated with Resin Infiltration

“…Samples were sectioned longitudinally and mounted on a glass cover slide. 8 The intended area of WSL was centered in the field of view and photographed at ×10 magnification using Polarized light microscopy (BX60, Olympus, Japan).…”

“…7 These treatments can effectively promote remineralization of the sub-surface lesions. 8,9 However, these agents are subject to dilution and washing out by saliva. 10 They also require time and patient compliance.…”

Evaluation of Remineralization Potential of Two Agents and pH Cycling on Early Enamel Lesions

“…The X-rays are then analyzed to provide details on the surface's elemental distribution "Calcium and phosphorous" ions concentrations. Then, they were converted into Ca/P ratio for each tested group (24) .…”