Effect of (CPP-ACP) and (CPP-ACP with fluoride) on Chemical and Morphological Analysis of Bovine Teeth Following Demineralization/Remineralization Cycles” An In-vitro Study

Abstract

Objective: To compare the remineralization outcome of CPP-ACP without or with fluoride "MI Paste" & "MI Paste Plus" on the re-mineralization of enamel caries-like lesions in bovine teeth.Material and methods: 21 clean sound bovine teeth were collected and prepared. All samples were mounted in acrylic molds. Randomization was done to divide them into 3 groups (n= 7 teeth); (GPI: Control Group no treatment; were not exposed to demineralization nor remineralizing agents only exposed to synthetic saliva, GPII: MI Paste and GPIII: MI Paste Plus. Each group was examined by (SEM) with magnifying range 6000,12000x and EDX analysis were taken place before demineralization, after 72 hrs of demineralization and after 21 days of remineralization. Data were analyzed by using Independent t-test to compare between different groups, while comparison between control, demineralization and remineralization was performed by using One Way ANOVA test followed by Tukey`s Post Hoc test for multiple comparisons.Results: Morphological changes of enamel surfaces of "MI Paste" and "MI Paste Plus" showed better appearance. For chemical analysis "MI Paste Plus" showed significantly better results than "MI Paste" with an increased amount of calcium & calcium/ Phosphate ratio. Conclusion:Results emphasize the positive effect of both pastes in enamel remineralization. Also, addition of fluoride in "MI Paste Plus" had a synergistically effect with the CPP-ACP and provided better remineralization.