Effectiveness of XP-Endo Finisher in the reduction of bacterial load in oval-shaped root canals

Abstract

This study investigated the effectiveness of XP-Endo Finisher (XPF) associated with XP-Endo Shaper (XPS) or Reciproc Blue (RB) files in reducing bacterial load in oval-shaped root canals (RC) during chemomechanical preparation (CMP) using 0.9% saline solution (NaCl) or 2.5% sodium hypochlorite (NaOCl). Eighty mandibular incisors with single oval-shaped RC were contaminated with Enterococcus faecalis. The teeth were randomly assigned to eight experimental groups (n = 10) according to the CMP, as follows: G1: XPS, G2: XPS + XPF, G3: RB, and G4: RB + XPF. CMP was performed with NaCl or NaOCl. The reduction of bacterial load was assessed by colony-forming unit count before (S1) and after (S2) CMP. Data normality was verified by using Shapiro-Wilk test. ANOVA, Tukey's test, and Bonferroni post-hoc test were used at a 5% significance level. Culturable bacteria were present in all S1 samples (p>0.05). All instrumentation techniques were effective in reducing bacterial load, irrespective of the irrigating solution (p < 0.05). With the use of NaCl, RB was more effective than XPS (p = 0.035). With the use of NaOCl, XPS and RB presented similar effectiveness (p = 0.779). XPF enhanced the bacterial reduction of both systems tested (p < 0.05). The use of NaOCl improved the CMP, irrespective of the instrumentation technique used (p < 0.05). In conclusion, XPS and RB files are effective in reducing bacterial levels in oval-shaped RC. The use of XPF as a method of agitation of the irrigating solution improved the cleaning efficiency of both file systems tested. Mechanical preparation performed with saline solution decreased culturable bacteria from the root canal, but antimicrobial substances such as NaOCl should be used to achieve a significantly better disinfection.

The influence of endodontic access cavity design on the efficacy of canal instrumentation, microbial reduction, root canal filling and fracture resistance in mandibular molars

“…2012, Carvalho et al . 2019). From S2 to S3, there was also a significant reduction in microbial load in all groups ( P < 0.05).…”

Comparison of antibacterial effectiveness of three rotary file system with different geometry in infected root canals before and after instrumentation–a double-blinded randomized controlled clinical trial

“…15,16 Various in vitro studies claimed that XP-endo Shaper induces minimal stress on dentinal walls, thereby preventing formation of new dentinal cracks and also effective in reducing microbial count levels in oval shaped canals. [17][18][19][20][21][22][23] However, there is lack of clinical studies to prove the effectiveness of three-dimensional rotary systems over traditional files. A recent systematic review of clinical trials (Siddique and Nivedhitha, 2019) demonstrated that there was no significant difference between rotary as well as reciproc system on microbial reduction.…”

Análise morfométrica de incisivos inferiores através de microtomografia computadorizada

“…Observando que o valor médio da distância vestíbulolingual dos canais ovais longo a 6mm do ápice radicular é de 1,04 mm (Tabela 2), nenhum instrumento do sistema Reciproc Blue ® seria capaz de tocar em todas as paredes, tendo em vista que a essa o distância (D6) o instrumento R25 possui é de 0,73 mm, o R40 0,76 mm e o R50 0,80 mm. Tal fato se reveste é de grande implicação clínica para a rotina endodôntica, uma vez que paredes não tocadas são locais propensos a presença ou persistência de microrganismos (Carvalho, et al, 2019). Ao preparar os canais classificados como ovais, ovais longos ou achatados, o operador deve se alertar para uma instrumentação mais crítica, reduzindo as possibilidades de preparo incompleto do canal radicular principal.…”