Effectiveness of using of brown algae alginate to immobilize the indigenous bioremediation bacteria for reducing waste water from shrimp culture

Abstract

Bacillus coagulans T1.2, an indigenous bacterium from shrimp ponds in East Lampung, has been found to reduce total ammonia nitrogen (TAN). The purpose of this study was to know the effectivity of immobilization of bacterial by sodium alginate from marine brown algae to reduce wastewater pollution from shrimp culture. The brown algae, Sargassum sp. and Padina sp., were collected from Pesisir Barat and Ketapang beach, Lampung. Alginate was extracted from both Sargassum and Padina by alkali method. The bacterial immobilization beads were made by mixed Bacillus coagulans suspension with alginate (1:3 v/v) and formed beads by 1,5 ml syringe without needle. In the in vitro study, the immobilized bacteria were submerged in artificial wastewater (2 beads ml-1) and incubated for 20 days. The control group includes the same bacteria without immobilization. The TAN was measured and the viability of bacteria was evaluated after the incubation period. The results showed that using alginate from Sargassum sp. and Padina sp. as a matrix of immobilization indigenous bacterial Bacillus coagulans effectively and significantly reduce the content of Total Ammonia Nitrogen (TAN) in wastewater. The viability of bacteria immobilized with the alginate of Sargassum sp. and Padina sp. better than the bacterial treatment without immobilization.