Effectiveness of purple led for inactivation of Bacillus subtilis and Escherichia coli bacteria in in vitro sterilizers

Yaqubi, Ahmad Khalil; Astuti, Suryani Dyah; Permatasari, Perwira Annissa Dyah; Komariyah, N.; Endarko, E.; Zaidan, Andi Hamim

doi:10.24931/2413-9432-2022-11-4-4-10

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…42 Yaqubi et al used a purple LED with a wavelength of 395 nm, achieving a remarkable 94.3% bacterial inactivation rate for E. coli through porphyrins. 43 Figure 8 elucidates the intricate mechanism underlying bacterial inactivation through photodynamic processes.…”

Section: Discussionmentioning

confidence: 99%

Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus

Yaqubi,

Astuti,

Zaidan

et al. 2023

J Lasers Med Sci

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Introduction: Living organisms, particularly humans, frequently encounter microorganisms like bacteria, fungi, and viruses in their surroundings. Silver nanoparticles are widely used in biomedical devices due to their antibacterial, antifungal, and antiviral properties. The study evaluates the efficacy of blue laser and silver nanoparticles from grape seed extract (AgNPs-GSE) in reducing gram-negative Escherichia coli and gram-positive Staphylococcus aureus bacteria causing infections. Methods: The sample consisted of four groups: a control without laser irradiation (T0), E. coli samples (A1 and A2) irradiated with a 405 nm diode laser at different times and concentrations of silver nanoparticles, and S. aureus samples (A3 and A4) irradiated with a 405 nm diode laser at different times and concentrations. Bacteria in groups A2 and A4 were treated with a photosensitizer (PS) made from grape seed extracts, incubated for 10 minutes, and then irradiated for 90, 120, 150, and 180 seconds. The samples were cultured on Tryptic Soy Agar (TSA) media, incubated at 37 °C, counted by using a Quebec colony counter, and analyzed using ANOVA and Tukey tests with a significance level of P<0.05. Results: The study found that 10 µl of AgNPs-GSE, when combined with exposure to a blue laser at 405 nm and a dose of 3.44 J/cm², can effectively photoinactivate E. coli and S. aureus bacteria. The addition of AgNPs-GSE to E. coli bacteria led to a significant reduction in their viability, with a reduction of 73.93%, 80.96%, and 83.80%, respectively. Similarly, when S. aureus bacteria were irradiated for 180 seconds by adding 1 mM, 1.5 mM, and 2 mM AgNPs-GSE, bacterial viability was reduced by 70.87%, 78.04%, and 87.01%, respectively. Conclusion: The findings from the present study indicate that at an energy density of 3.44 J/cm², it was possible to inactivate E. coli by 83.80% and S. aureus by 87.01%.

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Section: Discussionmentioning

confidence: 99%

Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus

Yaqubi,

Astuti,

Zaidan

et al. 2023

J Lasers Med Sci

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Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm

Permatasari

Astuti

Yaqubi

et al. 2023

Biomedical photonics

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In this study, the efficacy of using Sauropus androgynus (L) Merr, a katuk leaf chlorophyll photosensitizer, to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm was investigated. A red and blue diode laser is used as the light source. The sample was split into four groups: a negative control group, a positive control group, a blue laser treatment group (B), and a red laser treatment group (R), both with and without the addition of katuk leaf chlorophyll 1.6 mg/ml, and with varying densities of laser energy exposure of 2.5 J/cm2, 5 J/cm2, 7.5 J/cm2, and 10 J/cm2. Laser exposure and chlorophyll photosensitizer were tested using ELISA and ANOVA. At an energy density of 10 J/cm2, the optimal bacterial mortality rate was obtained in each treatment group. Namely, in the Aggregatibacter actinomycetemcomitans biofilm, the negative group, the number of deaths was 73.30% using a blue diode laser and 63.25% using a red diode laser. In the positive group, the number of deaths was 86.12% using a blue diode laser and 83.29% using a red diode laser. In the Enterococcus faecalis biofilm, in the negative group, the number of deaths was 67.78% using the blue diode laser and 75.33% using the red diode laser, and in the positive group, the number of deaths was 71.71% using the blue diode laser and 86.41 using a red diode laser. Exposure to blue and red diode lasers activates chlorophyll in katuk leaves, killing bacteria and reducing biofilms.

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Effectiveness of purple led for inactivation of Bacillus subtilis and Escherichia coli bacteria in in vitro sterilizers

Cited by 2 publications

References 20 publications

Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus

Blue Laser-Activated Silver Nanoparticles from Grape Seed Extract for Photodynamic Antimicrobial Therapy Against Escherichia coli and Staphylococcus aureus

Effectiveness of katuk leaf chlorophyll (Sauropus androgynus (L) Merr) with blue and red laser a ctivation to reduce Aggregatibacter actinomycetemcomitans and Enterococcus faecalis biofilm

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