Effectiveness of nanoparticles solutions and conventional endodontic irrigants against Enterococcus faecalis biofilm

Almeida, Josiane de; Cechella, Bruna Casagrande; Bernardi, Anarela Vassen; Pimenta, Aparecida Linhares; Felippe, Wilson Tadeu

doi:10.4103/ijdr.ijdr_634_15

Cited by 37 publications

(33 citation statements)

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“…NaOCl в высоких концентрациях имеет большое количество недиссоциированной хлорной кислоты (HClO) в растворе, что непосредственно связано с его эффективностью в отношении микроорганизмов, вследствие чего можно предположить, что даже длительная экспозиция низких концентраций гипохлорита будет не столь активно действовать на резистентные микроорганизмы. Низкие результаты в плане тормо-Том 18, № 4/2020 Обзор / Reviews жения роста и гибели МО, полученные при использовании 1% NaOCl, вероятно, были обусловлены низкой концентрацией HClO, что способствовало сопротивлению штаммов E. Faecalis, организованных в виде структурированной биопленки [20].…”

Section: гипохлорит натрияunclassified

“…Трехнедельный качественный анализ биопленки показал сходное ингибирование роста бактерий при использовании 3% гипохлорита натрия, 2% хлоргексидина и хитозана, по сравнению с группой физиологического раствора, который показала наличие роста бактерий [23]. Также, сходные выводы авторов были продемонстрированы в некоторых работах, и говорили об одинаковой антимикробной активности 2% хлоргексидина и 5% или 5,25% NaOCl [18,20].…”

Section: гипохлорит натрияunclassified

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Analysis of bactericidal effects of sodium hypochlorite and chlorhexidine on resistantbiofilm microorganisms (E. Faecalis, C. Albicans).

et al. 2021

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Aim. To analyze current data on the bactericidal efficacy of endodontic irrigants, in particular, sodium hypochlorite and chlorhexidine in different concentrations relative to the elimination of resistant microorganisms (E. Faecalis, C. Albicans). Irrigation is an important stage in the treatment of root canals, it prevents the further contamination of the canals with microorganisms. However, irrigants do not always cope with this function, which leads to the occurrence of a secondary infection.Materials and methods. The study of publications was produced in the electronic databases such as Google Scholar, PubMed and ScienceDirect in the course of a systematic review of the literature. Included articles contain information about various types of irrigants and their antimicrobial effect.Results. 73 articles were viewed during the review. After analyzing the literature for inclusion criteria, the total number of publications has become 41.Conclusions. According to literature data, irrigation solutions in widely used by clinicians concentrations (sodium hypochlorite 3%, chlorhexidine 2%) are not able to completely eliminate E. faecalis and C. albicans from the root canal system.

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Section: гипохлорит натрияunclassified

Analysis of bactericidal effects of sodium hypochlorite and chlorhexidine on resistantbiofilm microorganisms (E. Faecalis, C. Albicans).

et al. 2021

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“…The apex of all specimens were made impermeable with two layers of epoxy adhesive (LOCTITE Pvt., Ltd., India). (16) To obtain a closed root canal system that mimicked clinical in vivo scenarios and to ensure easy handling, a customized model was fabricated for each tooth by mounting into a 1.5-mL polypropylene Eppendorf tube (Eppendorf Private Limited, India) filled with silicone rubber impression material. 17These specimens were then autoclaved at 121 °C, 15 lbs for 30 minutes.…”

Section: Specimen Preparationmentioning

confidence: 99%

Nano-Engineered Irrigating Solutions and Lasers – An Antibacterial Study Against Enterococcus Faecalis

Balagopal

James

Sebatni

et al. 2019

IJMBS

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BACKGROUND: Enterococcus faecalis (E. faecalis) is the most commonly detected micro-organism in asymptomatic and persistent endodontic infections. Thorough disinfection of the root canal is more important than proper shaping for a successful endodontic treatment. This study is an attempt to implement nanoparticles which have proven anti-bacterial efficacy as irrigating solutions against E. faecalis. AIM: To compare and evaluate the anti-bacterial efficacy of 5% sodium hypochlorite (NaOCl), 0.2% chitosan nanoparticles solution (ChNP) and 0.01% silver nanoparticles solution (AgNP) against E.faecalis with and without diode laser activation. MATERIALS AND METHODS: 70 single rooted mandibular premolars were included in this study. Access opening, working length determination and biomechanical preparation were standardized. Samples were embedded in putty material inside an eppendorf tube to simulate periodontal ligament and autoclaved followed by inoculation of E.faecalis (MTCC 439) and incubated at 37℃ for 7 days. The samples were divided into 7 groups (10 samples in each group). Group 1- No treatment (Negative control), group 2 - 5% NaOCl without activation (Positive control), group 3 - 5% NaOCl with diode laser activation, group 4- 0.01% AgNP without activation, group 5- 0.01% AgNP with diode laser activation, group 6- 0.2% ChNP without activation and group 7- 0.2% ChNP with diode laser activation. A final rinse of the respective irrigants and activation were performed, followed by counting the colony forming units. Statistical analysis used were one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc tests. Results showed that diode laser in combination with 5% NaOCl and 0.2% ChNP had significant effects in the reduction of microbial colony counts of E. faecalis in comparison to other experimental groups. Keywords: Nanoparticles, Irrigation, LASER, Enterococcus faecalis

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“…[20][21][22][23][24][25] On the other hand, AgNPs are the most common metallic nanomaterials used for the control of several types of microorganisms due to their very well-known antimicrobial properties even in drug-resistant microorganisms, 15,18,26,27 including the E. faecalis strain. 18,[28][29][30][31][32] Although several studies have determined the antimicrobial activity of chitosan and silver compounds as nanoscaled materials against E. faecalis, mainly used in combination with irrigant agents or endodontic sealers during endodontic therapies. There are not available works that have evaluated, through standardized methods based on microbiological assays by using direct contact procedures, the synergistic bactericidal activity of CsNPs and AgNPs.…”

Section: Introductionmentioning

confidence: 99%

“…20–25 On the other hand, AgNPs are the most common metallic nanomaterials used for the control of several types of microorganisms due to their very well-known antimicrobial properties even in drug-resistant microorganisms, 15,18,26,27 including the E. faecalis strain. 18,28–32…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of endodontic sealers and medications containing chitosan and silver nanoparticles against Enterococcus faecalis

Loyola-Rodríguez

Méndez

Espinosa-Cristóbal

et al. 2019

Journal of Applied Biomaterials & Functional Materials

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Background: The main microorganism associated with the failure of endodontic treatments is Enterococcus faecalis. Although several endodontic therapeutics have demonstrated antimicrobial activity against E. faecalis, the antimicrobial effectiveness of chitosan (CsNPs) and silver nanoparticles (AgNPs) included into conventional endodontic sealers for endodontic therapies is still unclear. Aim: The objective of this study was to evaluate the antibacterial activity increment (AAI) of endodontic sealers containing CsNPs and AgNPs as well as some chemical components against E. faecalis by direct contact assays. Methods: CsNPs and AgNPs were synthesized by reduction and ionic gelation methods, respectively. Nanoparticles were characterized by dynamic light scattering and energy dispersive X-ray analysis. The bactericidal activity was tested on monolayers on agar plates and collagen membrane surface assays against E. faecalis. Results: The size of CsNPs was 70.6±14.8 nm and zeta potential was 52.0±5.4 mV; the size of AgNPs was 54.2±8.5 nm, and zeta potential was –48.4±6.9 mV. All materials, single or combined, showed an AAI, especially when CsNPs, chlorhexidine (Chx), and the combination of CsNPs-Chx were added. However, the combination of CsNPs-Chx showed the highest (55%) AAI, followed by Chx (35.5%) and CsNPs (11.1%), respectively. There was a significant statistical difference in all comparisons ( p < 0.05). Tubliseal (40%) and AH Plus (32%) sealants showed a higher AAI on E. faecalis in the monolayer test and collagen membrane assay analyzed by scanning electron microscopy. Conclusions: Tubliseal and AH plus sealers combined with nanoparticles, especially CsNPs-Chx, could be used for conventional endodontic treatments in the control of E. faecalis bacteria.

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Effectiveness of nanoparticles solutions and conventional endodontic irrigants against Enterococcus faecalis biofilm

Abstract: 1% Ag Np and 26% ZnO Np were effective against E. faecalis biofilm similarly to conventional endodontic irrigants.

Cited by 37 publications

References 1 publication

Analysis of bactericidal effects of sodium hypochlorite and chlorhexidine on resistantbiofilm microorganisms (E. Faecalis, C. Albicans).

Analysis of bactericidal effects of sodium hypochlorite and chlorhexidine on resistantbiofilm microorganisms (E. Faecalis, C. Albicans).

Nano-Engineered Irrigating Solutions and Lasers – An Antibacterial Study Against Enterococcus Faecalis

Antimicrobial activity of endodontic sealers and medications containing chitosan and silver nanoparticles against Enterococcus faecalis

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