Effectiveness of muscle basal lamina carrying neural stem cells and olfactory ensheathing cells in spinal cord repair

Xw, Kang; Jl, Hu; Sk, Wang; Wang, J

doi:10.4238/2015.october.28.5

Cited by 9 publications

(8 citation statements)

References 36 publications

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“…Only 28 articles reported sufficient data to perform a statistical test, including subgroup evaluation ( Fig. 1 ) [ 2 3 4 5 6 7 14 15 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 ]. Besides effect size preparation from these 28 studies, other useful data such as the most popular model, the location of injuries and types of stem cell also extracted from the articles and they are shown in Figs.…”

Section: Resultsmentioning

confidence: 99%

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

et al. 2018

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Spinal cord injury is a significant cause of motor dysfunctions. There is no definite cure for it, and most of the therapeutic modalities are only symptomatic treatment. In this systematic review and meta-analysis, the effectiveness of stem cell therapy in the treatment of the spinal cord injuries in animal models was studied and evaluated. A systematic search through medical databases by using appropriate keywords was conducted. The relevant reports were reviewed in order to find out cases in which inclusion and exclusion criteria had been fulfilled. Finally, 89 articles have been considered, from which 28 had sufficient data for performing statistical analyses. The findings showed a significant improvement in motor functions after cell therapy. The outcome was strongly related to the number of transplanted cells, site of injury, chronicity of the injury, type of the damage, and the induction of immune-suppression. According to our data, improvements in functional recovery after stem cell therapy in the treatment of spinal cord injury in animal models was noticeable, but its outcome is strongly related to the site of injury, number of transplanted cells, and type of transplanted cells.

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Section: Resultsmentioning

confidence: 99%

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

et al. 2018

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“…Because of the similarity between the tubular matrix of skeletal muscles (the basal membrane) and the endoneurial tubes of a damaged nerve, it is possible to use autologous muscle grafts as a favorable biological conduit for nerve repair (Kang et al, 2015). Therefore, the sheaths of skeletal muscle were employed as a guidance channel in repairing of nerve injury in the clinical and experimental research (Meek, Varejão, & Geuna, 2004;Pereira et al, 1996;Sanes, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Polyvinylidene fluoride channel as a conduit for bridging the peripheral nerves defects with nerve growth factor (NGF) and collagen gel could provide a favorable matrix for axonal regrowth (Delaviz et al, 2011). The basal membrane could stimulate and create guidance channels for regrowth of axons toward the target tissue (Kang, Hu, Wang, & Wang, 2015). It has been shown that muscle basal lamina grafts can be a guidance route for the injured axons toward the distal segment of the endoneurial tubes (La Fleur, Underwood, Rappolee, & Werb, 1996).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Muscle Graft with Nerve Growth Factor and Laminin on Sciatic Nerve Repair in Rats

Jafari

Delaviz

Torabi

et al. 2018

Basic Clin. Neurosci. J.

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Peripheral nerve injury is one of the most common damages that lead to physical disability. Considering the similarity between the coatings of skeletal muscles and nerve fibers, we conducted this research to determine the effect of muscle graft with Nerve Growth Factor (NGF) and Laminin (L) on nerve repair. Methods: We cut a 10-mm length of the sciatic nerve from 42 female Wistar rats (Weight: 200±250 g) and equally divided the rats into three groups. In the muscle graft+NGF+laminin group, the degenerated skeletal muscle was sutured with proximal and distal ends of the transected sciatic nerve. Then, NGF (100 ng) and laminin (1.28 mg/mL) were injected into the muscle graft. In the muscle graft group, normal saline was injected into the muscle graft. In the control group, 10 mm of the sciatic nerve was removed without any treatment. Functional recovery was assessed based on Sciatic Functional Index (SFI). Also, tracing motor neurons and histological studies were performed to evaluate nerve repair. The obtained data were analyzed by ANOVA test. Results: The Mean±SD SFI value significantly increased in the muscle graft+NGF+laminin (-76.6±2.9) and muscle graft (-82.1±3.5) groups 60 days after the injury compared to the control group. The Mean±SD number of labeled motor neurons significantly increased in the muscle graft+NGF+laminin (78.6±3.1) and muscle graft (61.3±6.1) groups compared to the control group (P<0.001). The mean number of myelinated axons in the distal segments of the muscle graft+NGF+laminin increased significantly compared to the muscle graft group. Conclusion: These findings suggest that muscle graft followed by NGF and laminin administration have therapeutic effects on nerve repair.

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“…The other study reported the transplanted cells surviving up to and beyond 2 weeks (no quantification was reported), and proposed a synergistic benefit of co-transplanting OECs with MSCs 89 . Three studies reported co-transplantation of OECs together with NSCs 47,58,80 , one study used ESMNs 28 , and one adipose tissue-derived stromal/stem cells 44 (ASCs). Only one out of the three studies that co-transplanted NSCs with OECs commented on cell survival, and concluded that co-transplantation could improve cell survival for NSCs 58 .…”

Section: Introductionmentioning

confidence: 99%

Survival and Integration of Transplanted Olfactory Ensheathing Cells are Crucial for Spinal Cord Injury Repair: Insights from the Last 10 Years of Animal Model Studies

2019

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Olfactory ensheathing cells (OECs), the glial cells of the primary olfactory nervous system, support the natural regeneration of the olfactory nerve that occurs throughout life. OECs thus exhibit unique properties supporting neuronal survival and growth. Transplantation of OECs is emerging as a promising treatment for spinal cord injury; however, outcomes in both animals and humans are variable and the method needs improvement and standardization. A major reason for the discrepancy in functional outcomes is the variability in survival and integration of the transplanted cells, key factors for successful spinal cord regeneration. Here, we review the outcomes of OEC transplantation in rodent models over the last 10 years, with a focus on survival and integration of the transplanted cells. We identify the key factors influencing OEC survival: injury type, source of transplanted cells, co-transplantation with other cell types, number and concentration of cells, method of delivery, and time of transplantation after the injury. We found that two key issues are hampering optimization and standardization of OEC transplantation: lack of (1) reliable methods for identifying transplanted cells, and (2) three-dimensional systems for OEC delivery. To develop OEC transplantation as a successful and standardized therapy for spinal cord injury, we must address these issues and increase our understanding of the complex parameters influencing OEC survival.

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Effectiveness of muscle basal lamina carrying neural stem cells and olfactory ensheathing cells in spinal cord repair

Cited by 9 publications

References 36 publications

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

Stem cell transplantation and functional recovery after spinal cord injury: a systematic review and meta-analysis

The Effect of Muscle Graft with Nerve Growth Factor and Laminin on Sciatic Nerve Repair in Rats

Survival and Integration of Transplanted Olfactory Ensheathing Cells are Crucial for Spinal Cord Injury Repair: Insights from the Last 10 Years of Animal Model Studies

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