Effectiveness of Moringa oleifera Leaves on TNF-α Expression, Insulin Levels, Glucose Levels and Follicle Count in Rattus norvegicus PCOS Model

Siahaan, Salmon Charles PT; Santoso, Budi; Widjiati, Widjiati

doi:10.2147/dmso.s385492

Cited by 9 publications

(9 citation statements)

References 37 publications

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“…Te plant's high fber led to a delay in gastric emptying [142]. Also, its contents of favonoids and many phenolic compounds induce hypoglycemic action [143,144]. Te plants contain a huge amount of hypoglycemic bioactive agents such as catechin, cyanidin, ellagic acid, luteolin, kaempferol, quercetin, rosmarinic acid, and rutin [145].…”

Section: Moringa Oleifera and Glucose Statusmentioning

confidence: 99%

“…Te supplementation of leaves and seeds potentially activated monoamine oxidase (MAO), adenosine triphosphatase (ATPase), adenosine deaminase (ADA), acetylcholinesterase (AChE), arginase, lactate dehydrogenase (LDH), and angiotensin-I converting enzyme (ACE) activities in diabetics [173]. Leaves potentially control both insulin levels and blood glucose levels in rats with polycystic ovaries [144].…”

Section: Moringa Oleifera and Glucose Statusmentioning

confidence: 99%

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Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Abdelazim,

Afifi,

Abu-Alghayth

et al. 2024

Journal of Food Biochemistry

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Plants could be used for multiple medicinal purposes. Moringa oleifera (MO) is considered the most famous plant used for this purpose. The present review aimed to spot the light on the recent medicinal, biochemical, and nutritional applications of MO. The plant contains a huge number of nutrients such as fatty acids, amino acids, proteins, polysaccharides, minerals, and vitamins. It has been used to control glucose, lipids, proteins, minerals, vitamins, hormones, and antioxidants in many animals and human models. Its medicinal applications are also varied and wide; it could be used to control and manage lots of disorders. Extracts and isolated ingredients from the plant open the way for many researchers all over the world to study its biochemical and medicinal impact on many experimental and clinical models. The present review spots the light on the recent medicinal and biochemical significance of MO opening the discussion and demonstrating the strengths and weaknesses in the MO research area. Regardless of the contrary concept, we considered MO a promising plant that could be studied for its medicinal applications on both biochemical and molecular levels. We recommended further research on the molecular effects of MO in healthy and diseased models.

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Section: Moringa Oleifera and Glucose Statusmentioning

confidence: 99%

Section: Moringa Oleifera and Glucose Statusmentioning

confidence: 99%

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Abdelazim,

Afifi,

Abu-Alghayth

et al. 2024

Journal of Food Biochemistry

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“…Research by Siahaan et al (2022) proved that M. oleifera leaves reduced IR and diabetes. Moreover, moringa extract is a powerful antioxidant that binds to reactive oxygen species (ROS) and limits oxidative stress in PCOS-induced rats.…”

Section: Lipid Peroxidation (Nm/ml)mentioning

confidence: 99%

“…Women with PCOS may have hyperinsulinemia, hyperlipidemia, and a high LH/FSH ratio that affects oocyte quality (Siahaan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Assessment of hepatoprotective, nephroprotective efficacy, and antioxidative potential of Moringa oleifera leaf powder and ethanolic extract against PCOS‐induced female albino mice (Mus Musculus)

Khalid,

Arshad,

Raza

et al. 2023

Food Science & Nutrition

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Moringa oleifera is a medicinal plant that has anti‐inflammatory, antihypertensive, antidiabetic, tissue‐protective, and antioxidant activities. Here, we evaluated the protective effect of M. oleifera leaf powder (MoLP) and 70% ethanol M. oleifera leaf extract (MoLE) on mitigating polycystic ovary syndrome (PCOS)‐induced liver and kidney dysfunction via regulating oxidative stress in female albino mice (Mus musculus). The efficacy of M. oleifera was compared with metformin (standard medicine used to treat infertility in women). PCOS was induced by intramuscular injection of testosterone enanthate at 1.0 mg/100 g BW for 35 days. PCOS‐induced mice were treated with MoLP (250 and 500 mg/Kg), MoLE (250 and 500 mg/kg), and metformin (250 mg/kg) orally for 14 days. Renal function test (RFT), liver function test (LFT), and oxidative stress biomarker malondialdehyde (MDA) were quantified in serum at 0, 7, and 14 days of intervention. Mice treated with M. oleifera and metformin showed a significant decrease (p < .001) in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphate (ALP), total bilirubin, urea, creatinine, and a significant increase (p < .001) in total protein, albumin, globulin, and albumin/globulin (A/G) ratio. Oxidative stress decreased significantly (p = .00) with respect to treatments, exposure days, and their interaction in metformin and all M. oleifera‐treated groups. M. oleifera leaf powder and extract reduce oxidative stress and enhance nephron‐hepatic activity in PCOS‐induced female albino mice.

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“…

We read closely and eagerly the recent article by Siahaan et al 1 The study used the leaves' ethanolic extract of Moringa oleifera to alleviate PCOS-related symptoms induced by Testosterone Propionate (TP). Even though we find the study interesting, we have some concerns worth discussing.In the study, the authors administered the TP (100 mg/kg BW) for 21 days through the intraperitoneal (i.p.)

…”

mentioning

confidence: 99%

Is Intraperitoneal Injection of Testosterone Propionate in Adult Animal Suitable to Study PCOS? [Letter]

Situmorang¹,

Widoretno²

2022

DMSO

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We read closely and eagerly the recent article by Siahaan et al. 1 The study used the leaves' ethanolic extract of Moringa oleifera to alleviate PCOS-related symptoms induced by Testosterone Propionate (TP). Even though we find the study interesting, we have some concerns worth discussing.In the study, the authors administered the TP (100 mg/kg BW) for 21 days through the intraperitoneal (i.p.) route. The use and route of administration of a dose at such a high concentration seem to be questionable, since numerous previous studies have used relatively low doses (10-30 mg/kgBW) of TP subcutaneously (s.c.). [2][3][4] In addition, it would have been informative if the authors explained the preparation of the TP in the article. Due to TP's low solubility in water-based solvents, oil-based solvents are commonly employed. The i.p. routes should not be used if the authors used an oil-based solvent since oil-based solvents can induce inflammation and apoptosis in the peritoneum. 5 As a consequence, there could be potential bias in interpretation of the results. This is because it is unclear whether the solvent or TP itself is responsible for the increase in inflammatory indices. Thus, a vehicle solvent-only control group is needed.PCOS induction by TP exposure is usually performed during the fetal or neonatal stage. 4,6 The authors, however, administered TP to adult rats (3 months old). To our knowledge, the group is the first to expose adult female animals to a high dose of TP in order to model PCOS. The article lacks an explanation and discussion of using this method to induce PCOS in adult animal, fetus, and newborn.To investigate the inflammatory response in the PCOS model, the authors assessed TNF-α expression in the ovary of the rats. However, TNF-α count was misrepresented in the article since the authors used Metabolite Insulin Receptor Substrate-1 or IRS-1 to represent TNF-α expression. In our opinion, this cannot be justified since TNF-α and IRS-1 have different properties and functions; thus, they cannot be used to represent each other. Further, protocols such as immunohistochemistry and ELISA assays to measure TNF-α and serum parameters are also absent. Due to the novelty of the method used to induce PCOS, reproducibility and repeatability are crucial.Lastly, daily food consumption data is needed to ensure that the decrease in serum glucose and insulin level in the treatment group was not due to lower food consumption, hence corroborating the study's conclusion.As a whole, this is an interesting study that deserves more discussion, particularly on the high dose of TP in adult rats and its potential translation to humans' PCOS.

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Effectiveness of Moringa oleifera Leaves on TNF-α Expression, Insulin Levels, Glucose Levels and Follicle Count in Rattus norvegicus PCOS Model

Cited by 9 publications

References 37 publications

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Assessment of hepatoprotective, nephroprotective efficacy, and antioxidative potential of Moringa oleifera leaf powder and ethanolic extract against PCOS‐induced female albino mice (Mus Musculus)

Is Intraperitoneal Injection of Testosterone Propionate in Adult Animal Suitable to Study PCOS? [Letter]

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