Effectiveness of Marine Fungal Symbiont Isolated from Soft Coral Sinularia sp. from Panjang Island as Antifungal

Putri, Didha Andini; Radjasa, Ocky Karna; Pringgenies, Delianis

doi:10.1016/j.proenv.2015.01.051

Cited by 14 publications

(7 citation statements)

References 25 publications

(19 reference statements)

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“…The homogenised tissues were serially diluted (10 −2 and 10 −3 ) with sea water. One ml/dish from each dilution were plated on petri dishes containing different culture media (Putri et al 2015 ).…”

Section: Methodsmentioning

confidence: 99%

“…More than 70% of the earth’s surface occupying by the oceans that support large habitats of living organisms which are considered as a source of a vast groups of structurally unique natural products. These natural products are mainly isolated from invertebrates common to ecosystems of coral reefs, such as tunicates, sponges, soft corals, molluscs and bryozoans (Putri et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

“…Marine-derived fungi have been known as a source of structurally novel metabolites of potent bioactivity (Bugni and Ireland 2004 ; Blunt et al 2006 ; Saleem et al 2007 ). The secondary metabolites produced by coral-associated fungi are of great interest (Putri et al 2015 ). The extract produced by many Aspergillus and Penicillium species are known to produce a large variety of active compounds, such as: Aspergillus versicolor isolated from soft coral Xestospongia exigua was found to be rich source of novel polyketides (Putri et al 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

et al. 2020

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Ten specimens of coral reefs were collected from the Red Sea in the Ein El-Sukhna region. Fungal isolation was done using two media, Dextrose Yeast Extract Agar (DYA) and Rose Bengal Agar (RBA). The morphological traits identified 18 fungal isolates belonging to the phyla Ascomycota, Mucoromycota and Deuteromycota. Five genera in three orders have been isolated: Eutrotiales (Aspergillus, Penicillium and Byssochlamys), Mucorales (Rhizopus) and Moniliales (Curvularia). The heat mapping clustering of the isolated fungi declared that Aspergillus and Penicillium were the most frequently isolate fungi in coral reefs. It was found that A. fumigatus colonised eight coral samples with 80% colonisation rate. Moreover, about 50% of the isolated fungal species were specific to one coral reef only such as A.candidus and A.carneus isolated from Isophyllastrea rigida only, A.japonicus and A.ochraceopetaliformis from Glaxaea fascicularis, A.niger van Tieghem from Porites astreoides, A.sydowii, A.terreus and P.waksmanii from Cladocora arbuscula, P.janthinellum from Pterogorgia guadalupensis and Curvularia tuberculata, Byssochlamys spectabilis and Rhizopus oryzae from Acropora humilis. Biological activities (antimicrobial, antioxidant antiradical and cytotoxicity) of the most predominant fungal species were investigated. The antimicrobial activity of coral fungal filtrates were investigated against six pathogenic bacteria including Escherichia coli ATCC11775, Neisseria gonorrhoeae ATCC19424, Pseudomonas aeruginosa ATCC10145, Streptococcus faecalis ATCC19433, Staphylococcus aureus subsp. aureus ATCC25923, Bacillus subtilis subsp. spizizenii ATCC6633 and two pathogenic yeast including Candida albicans ATCC7102 and Candida parapsilosis ATCC22019. Most of these fungal filtrates exhibited moderate to high antibacterial activities against both gram positive and gram negative bacteria, however it showed relatively low bioactivity towards the pathogenic Candida species. Investigating the free radical scavenging activity using DPPH reagent showed low to moderate bioactivities. The highest cytotoxic activity against liver cancer cell line Hep-G2 with an IC 50 values of 18.8 µg/ml was exhibited by Aspergillus ochraceopetaliformis MN083316 and a metabolomics study was done on the ethyl acetate extract of this strain using LC-ESI-MS fingerprints leading to the isolation and purification of compound 1. Using 1D and 2D NMR techniques compound 1 was identified as ditryptophenaline. Compound 1 exhibited a strong antimicrobial, antioxidant activities as well as cytotoxic activities against MCF-7 and HEPG2 with IC 50 values of 5.8 and 7.6 mmole, respectively.The objective of this study, isolation of Coral-reef associated fungi and studying their biological activities to produce the most active secondary metabolite which might possess a novel biological activity. ARTICLE HISTORY

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

et al. 2020

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“…It can be concluded that the terpenoid likely contributes as an antibacterial agent. Some of the terpenoid compounds have been reported to function as antimicrobials 23 . Terpenoid is rarely isolated from bacteria, but the extensive result of bacterial genomic analysis identifies many genes of terpene synthases involved in terpenoid biosynthesis 24 .…”

Section: Fig 1: Tlc-bioautogram Of the Extract Of De-fatted 1/10zgwsmentioning

confidence: 99%

Untitled

2019

IJPSR

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The emergence of antibiotic-multidrug resistant microorganisms is an essential issue in the health sector. Consequently, the exploration of new antimicrobial compounds from many promising resources is urgently required. Soft coral-associated bacteria are potential as antimicrobial compound producers. This study aims to get the best medium and fermentation conditions in the production of secondary metabolites having antibacterial activities and to identify antibacterial agents from a bacterium associated with soft coral. A soft coral sample was collected from Randayan Island, Indonesia, and was identified morphologically as Sarcophyton sp. One of the bacteria isolated from the Sarcophyton sp. showed antibacterial activities and identified as Bacillus sp. based on Bergey's Manual of Determinative Bacteriology. The best antimicrobial activities of Bacillus sp. DA11 were carried out on medium 1/10 Zobell supplemented with 1% of glucose (1/10ZG) which were able to against all of the test bacteria except on a test fungus, C. albicans. The extract was prepared on medium 1/10ZG with liquid state fermentation without shaking showed the best condition to produce secondary metabolites having antibacterial activities. The extract showed weak antibacterial activities based on minimum inhibitory concentrations (MICs) which were around 150-300 µg/disc for A. hydrophila; Bacillus sp., C. freundii, Salmonella sp., P. aeruginosa, S. aureus, E. coli, V. cholerae, V. vara, B. subtilis. The results of phytochemical analysis and thin layer chromatography (TLC)-bioautography showed that the secondary metabolite having antibacterial activities contained in the extract was a terpenoid. INTRODUCTION: Infectious diseases are caused by human pathogenic microorganisms such as Staphylococcus aureus, Streptococcus, Pseudomonas aeruginosa, Klebsiella pneumonia, etc. The diseases can be treated using antibiotics, but the issue of antibiotic-resistant microorganism crisis has driven scientists to explore novel antibiotics from various sources, for instance, from bacteria associated with soft coral.

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“…While Sn1 isolate had white color, thickened and spread, the whole of isolates had fibrous hyphae. There were obtained 15 fungus isolates from Sinularia sp and two of them had potential as antifungal [16].…”

Section: Endophyte Fungal Of Soft Coralmentioning

confidence: 99%

Endophyte microbial characteristic of soft corals Lobophytum sp and Sinularia sp collected from Maspari Island waters, South Sumatera

Rozirwan¹,

Hendri

Apri

2018

IJEMS

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Soft corals have bioactive compounds to potential as marine natural products, but the over exploitive to destroy of that ecosystem. Therefor endophyte microbial isolation can be effort to prevent that matters. This research aimed to isolate and characteristic on the entophyte microbial of soft coral Lobophytum sp and Sinularia sp that collated from Maspari island waters. Methodology of research was establishing growth of microbial samples (bacterial and fungal), isolation and characterization. Total of bacteria colony of Lobophytum sp were obtained about five isolates, and Sinularia sp were about four isolates. The macroscopic characteristic showed that whole bacteria had white colors. Those colonies had undulate, entire and curl (the edge of colony) and circular and irregular (for colony shape). For fungal of Lobophytum sp were obtained about three isolates, while Sinularia sp had only one isolate. The fungal colonies macroscopic characteristic showed yellow, green and white color, while shaped and edges colonies were thickened. Spread, thin, round, dark, and the whole of isolates had filamentous hyphae.

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Effectiveness of Marine Fungal Symbiont Isolated from Soft Coral Sinularia sp. from Panjang Island as Antifungal

Cited by 14 publications

References 25 publications

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

Biological activities and variation of symbiotic fungi isolated from Coral reefs collected from Red Sea in Egypt

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Endophyte microbial characteristic of soft corals Lobophytum sp and Sinularia sp collected from Maspari Island waters, South Sumatera

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