Endophyte microbial characteristic of soft corals Lobophytum sp and Sinularia sp collected from Maspari Island waters, South Sumatera

Abstract

Soft corals have bioactive compounds to potential as marine natural products, but the over exploitive to destroy of that ecosystem. Therefor endophyte microbial isolation can be effort to prevent that matters. This research aimed to isolate and characteristic on the entophyte microbial of soft coral Lobophytum sp and Sinularia sp that collated from Maspari island waters. Methodology of research was establishing growth of microbial samples (bacterial and fungal), isolation and characterization. Total of bacteria colony of Lobophytum sp were obtained about five isolates, and Sinularia sp were about four isolates. The macroscopic characteristic showed that whole bacteria had white colors. Those colonies had undulate, entire and curl (the edge of colony) and circular and irregular (for colony shape). For fungal of Lobophytum sp were obtained about three isolates, while Sinularia sp had only one isolate. The fungal colonies macroscopic characteristic showed yellow, green and white color, while shaped and edges colonies were thickened. Spread, thin, round, dark, and the whole of isolates had filamentous hyphae.

Isolasi dan aktivitas antibakteri jamur endofit pada mangrove Sonneratia alba dari Tanjung Carat Kabupaten Banyuasin Sumatera Selatan

“…Suspensi bakteri uji yang telah disiapkan pada media NB sebanyak 1 ml kemudian dituang pada cawan petri steril secara aseptik. Tambahkan 10 ml media NA kemudian digoyangan secara perlahan sebanyak 10 kali ke kiri dan 10 kali ke kanan untuk mendapatkan suspensi bakteri yang merata [15].…”

“…Pengujian dilakukan sebanyak tiga kali pengulangan. Inkubasi selama 24 jam dengan suhu 37oC kemudian ukur diameter yakni zona bening yang terbentuk disekitar kertas cakram menggunakan jangka sorong Modifikasi [13,15,23].…”