Effectiveness of Lactobacillus sp (AMET1506) as Probiotic against Vibriosis in Penaeus monodon and Litopenaeus vannamei Shrimp Aquaculture

Abstract

Vibriosis is the one of the major pathogenic bacterial disease in shrimp aquaculture. Improving the health status of culture organisms using beneficial microbes as probiotic is the better method to control the pathogens. In this present study the Lactobacillus sp AMET1506 (Which shows strongest antagonistic activity against pathogenic bacteria such as, E.coli, V. cholerae, V.parahaemolyticus, Salmonella sp. and Shigella sp) was previously isolated from curd sample. While checking the antibacterial activity of Lactobacillus sp (AMET1506) against V.harveyi maximum inhibition activity was observed. So, the strain was potentially chosen and it was incorporated in shrimp feed by standard method. A total of 400 Penaeus monodon and Litopenaeus vannamei (each 200) shrimps larvae were obtained from a commercial shrimp hatchery located in Marakanam, Kanchipuram District, Tamil Nadu, India. After acclimation of seven days, the average weights of the shrimps were divided into twelve 50 L plastic tanks each containing 25 juvenile shrimps. The experimental tanks were treated with feed supplemented with 10 6 CFU g-1 of Lactobacillus sp (AMET1506), and the control tanks were fed with a control diet. Shrimp in all the groups were fed twice daily at 5.0% of biomass and the water temperature was maintained at 28 ± 1°C. After 30 days of culture, shrimp in all the control and experimental tanks were exposed to V. harveyi (10 5 CFU ml-1) for 10 days. During the experiment, the accumulated mortality of the shrimp and the microbial load in the shrimp and culture water was recorded. Among that, the shrimp P.monodon treated with Lactobacillus sp AMET1506 resulted in 6% final mortality as compared to 80% in the control group and in L.vannamei treated with Lactobacillus sp AMET1506 resulted in 12% final mortality as compared to 100% in the control group. Based on these results, the work has suggested to use this potential strain Lactobacillus sp AMET1506 as a probiotic in shrimp aquaculture feeds to improve the shrimp microbiota (GIT) and also to control the vibriosis in shrimp aquaculture.

Efficacy of Probiotic and Nitrifier Bacterial Consortium for the Enhancement of Litopenaeus Vannamei Aquaculture

“…The physico-chemical parameters such as temperature, transparency, dissolved oxygen, pH, nitrite, nitrate, ammonia of water were estimated by following the methods suggested in Golterman and Clymo; Wetzel and Likens; APHA [14][15][16].…”

Attenuation of Negative Impacts by Micro Algae and Enriched Artemia salina on Penaeus monodon and Litopenaeus vannamei Larval Culture

“…nauplii, zoea, mysis and post larvae) and the Vibrio sp., load was enumerated by growth on TCBS agar (Karthik et al, 2014).…”

“…In normal, diseases in aquaculture practices are mostly caused by luminous bacteria Vibrio harveyi and it has been referred to as the largest economic loss in the shrimp aquaculture due to mass mortalities (Sivakumar et al, 2014;Karthik et al, 2014). Furthermore, in general pathogenic microbes always remain in the water as opportunistic pathogens and they attack the larvae only when the larvae are weak due to environmental stress or nutritional deficiency (Soundarapandian and Babu, 2010).…”

The role of probiotics in the control of bacterial diseases and biodegradation of organic matter in shrimp (Penaeus vannamei) culture ponds of South India

“…During the last few years white spot syndrome virus (WSSV) disease has spread worldwide and caused tremendous economic loss in shrimp culture particularly in Asia [4]. Due to the continuous outbreak of this WSSV disease in Penaeus monodon culture leading to loss of shrimp culture in India, the farmers are seriously looking for alternative shrimp species for culture [5]. Cyanobacterial growths in ponds leads to the algal degradation due to the poor water quality.…”