Effectiveness of Copper Nanoparticles in Wound Healing Process Using In Vivo and In Vitro Studies: A Systematic Review

Abstract

Chronic wounds are defined as wounds that do not heal in an orderly and timely manner through the various stages of the healing process. Copper nanoparticles are essential in dressings for wound healing because they promote angiogenesis and skin regeneration, which hasten the healing process. This systematic investigation sought to explain how copper nanoparticles affect chronic wound healing in vivo and in vitro. We realized a systematic review of original articles studying the effectiveness of copper nanoparticles in the healing process of chronic wounds. The protocol was registered in the PROSPERO database. Several databases were searched between 2012 and January 2022 for English-language papers using MeSH terms and text related to chronic wounds, copper nanoparticles, and wound healing. Quality was evaluated using National Institute for Health and Care Excellence methodology and PRISMA guidelines. We looked at a total of 12 primary studies. Quantitative data were gathered and presented in all studies. Our results suggest that copper nanoparticles could have an excellent healing property, facilitating the liberation of growth factors that help the anti-inflammatory process of the wound and significantly improving antibacterial and antioxidant activities. In addition, copper presents a higher biocompatibility than other metallic ions, promoting regeneration and increasing skin quality.

Sustainable Nanomaterials for Biomedical Applications

“…Compared to traditional cotton-based hemostatic wound dressings, nanocellulose can be functionalized to promote wound healing and prevent secondary infection. Antimicrobial agents such as benzalkonium chloride, silver, and copper nanoparticles can be impregnated into the porous network of BNC’s membrane that acts as a physical barrier between the wound and the surrounding environment and facilitates the steady release of preloaded antimicrobials [ 59 , 60 , 61 , 62 , 63 ]. Furthermore, BNC can be integrated with other naturally derived materials to design next generation innovative sustainable nanomaterials.…”

Green synthesis of copper nanoparticles using Artemisia annua aqueous extract and its characterization, antioxidant and burn wound healing activities

“…This result is in agreement with the previous studies. Sandoval et al [5] reported that CuNPs can accelerate in vivo wound healing by stimulating angiogenesis and broblast cell proliferation. Copper nanoparticles synthesized using Allium saralicum leaves showed better wound-healing e cacy by enhancing the wound contracture, hydroxyproline, hexosamine, hexuronic acid, and brocytes content [34].…”

“…Additionally, copper is known to stabilize the hypoxia-inducible factor-1 (HIF-1) structure, which induces the expression and function of the vascular endothelial growth factor (VEGF) toward angiogenesis in wounds [6,35]. The hydrogel composite based on gelatin, HA, Asiatic acid, ZnO and CuO nanoparticles declined the in ammatory marker of TNF-α levels on the 7th day experiment in second degree burn wounds of rats [5]. Mirbehbahani et al [15] reported that application of Artemisia annua to the polymeric wound dressing stimulated broblast attachment and proliferation without any cytotoxicity.…”

“…Copper is recognized as a transitional metal with the unique ability to adopt different reduction states [2,3]. It plays a physiological role in the dermal wound healing by stimulating collagen, integrins, and bronectin synthesis [4,5]. In fact, the required processes for wound healing, including MMP expression, re-epithelialization, ECM remodeling, and angiogenesis, are all facilitated by copper [2,3].…”

A Cu@ZIF-8 encapsulated antibacterial and angiogenic microneedle array for promoting wound healing

“…Copper has been demonstrated to have effective antibacterial properties and pro-angiogenic effects in wound healing. [24][25][26] Zinc ions are also known to contribute to antibacterial properties and wound healing. [27][28][29] Recently, copper-and zinc-based zeolitic-imidazolate frameworks (Cu@ZIF-8) have been reported to have an excellent antibacterial effect on the bone repair model.…”