Effectiveness of chlorogenic acid supplementation on VEGF serum and placental MAP kinase expression in carbon Black-Exposed pregnant rattus norvegicus

Hendrawan, Viski Fitri; Wulansari, Desi; Oktanella, Yudit; Widjiati, Widjiati

doi:10.5958/0974-360x.2018.00340.2

Cited by 6 publications

(6 citation statements)

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“…Moreover, in the filtration of macromolecules in the kidney, neo-angiogenesis in the end organs in case of hypoxia and regulating blood-retinal barrier [35]. The current study reveals that VEGF can be an important factor prompting complications [36]. Furthermore, the characteristics of the study contributors based on categories of different types of dyslipidemia showed strong association with AD risk [22][37].…”

Section: Resultsmentioning

confidence: 91%

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RANTES as a novel biomarker for atherogenic dyslipidemia and metabolic disturbances in patients with Type 2 Diabetes

et al. 2021

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RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted) is a chemokine engaged in the pathophysiology of diabetes type 2 (T2DM), related cardiovascular complications, dyslipidemia and hypertension that are major modifiable risk factors of T2DM. VEGF (Vascular endothelial growth factor) plays a role in diabetic vascular complications. Atherogenic dyslipidemia (AD) is a lipid aberration defined as the incidence of raised triglycerides (TG) and dimished high-density lipoprotein cholesterol (HDL-C) and associated with residual cardiovascular risk. However, the association between chemokine and type 2 diabetes (T2DM) and metabolic disturbances is still unclear, and few data are available. The aim of this study was to estimate serum RANTES, serum lipid composition, VEGF, and metabolic syndrome (MS) in patients with T2DM and elucidate their relationship in a sample of Egyptian premenopausal women. Serum level of RANTES, VEGF, lipids and body composition were assessed in 100 premenopausal women with T2DM (mean age 35 years old) and 100 healthy controls. Significant increase in serum RANTES level and VEGF was observed in T2DM compared to the control group. Positive correlations between elevated RANETS and increased values of VEGF, body mass index (BMI), waist circumference (WC), fat mass, LDL-C, TG, total cholesterol and presence of MS were observed in T2DM patients. Elevated RANTES concentration is associated with AD risk, abnormal metabolic components and VEGF levels. This study pinpoints the importance of RANTES as a novel biomarker for dyslipidemia and metabolic disturbance in T2DM patients.

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Section: Resultsmentioning

confidence: 91%

“…The study group composed of 100 premenopausal T2DM women and 100 control normal healthy women matched age (aged [29][30][31][32][33][34][35][36][37][38]. This study was conducted during February 2019 and March 2020 in the National Research Centre, Egypt; Medical Research Centre of Excellence.…”

Section: Methodsmentioning

confidence: 99%

RANTES as a novel biomarker for atherogenic dyslipidemia and metabolic disturbances in patients with Type 2 Diabetes

et al. 2021

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“…T1 : Treatment group 1, namely rats exposed to particulate soot at a concentration of 1,064 mg/m 3 for 8 hours and given C. longa at a dose of 1 mg/kg bw ( Ramírez-Tortosa et al , 1999 ; Quiles et al , 2002 ; Aulia, 2017 ; Hendrawan et al , 2018 ; Juliprihanto et al , 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…The dose of exposure to soot particulates comes from the effective dose according to previous research reports, namely a concentration of 1,064 mg/m 3 for 8 hours ( Hougaard et al , 2008 ; Dachlan et al , 2011 ; Hendrawan et al , 2018 ; Juliprihanto et al , 2019 ). While the oral dose of C. longa is the effective dose in experimental rabbits that have been converted to rats at 1, 2, and 3 mg/kg bw ( Ramírez-Tortosa et al , 1999 ; Quiles et al , 2002 ; Aulia, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Aminuddin¹,

Sargowo²,

Sardjono³

et al. 2023

Open Vet J

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Background: Particulate Matter (PM) is one of the important components in air pollution that can cause endothelial vascular dysfunction through exacerbation of atherosclerosis and inflammation of the respiratory system. Increased levels of MDA in blood plasma can be an indicator of oxidative stress. Then, macrophages can secrete proinflammatory cytokines will stimulate immune cells and vascular endothelial cells to release inflammatory cytokines, such as interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α). Curcuma longa works by scavenging the active free radicals involved in the peroxidation process. Aims: This study aims to prove that the administration of Curcuma longa can reduce MDA, TNF-α, and IL-6 levels in Rattus novergicus exposed to soot particulates. Methods: The subjects of this study were 30 male rats which were divided into 5 treatment groups with the following: (C0): negative control; (C+): positive control; (T1):Treatment group 2, rats exposed to particulate soot at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 1 mg/kg bw; (T2): Treatment group 3 was rats exposed to soot particulates at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 2 mg/kg bw; (T3) :Treatment group 4 was rats exposed to soot particulates at a concentration of 1064 mg/m3 for 8 hours and given Curcuma longa at a dose of 3 mg/kg bw. Giving the Curcuma longa extract orally with a probe every day for 30 days after treatment of exposure to soot. Examination of MDA, TNF-, and IL-6 levels with the ELISA method. Results: The administration of Curcuma longa can reduce MDA while the lowest MDA levels were obtained in the T3 treatment with an average of 1.542 ± 0.231. The results of the description of the lowest levels of TNF-α were obtained in the C-treatment with an average of 55.981 ± 4.689. Then, the lowest levels of IL-6 were obtained in the C-treatment with an average of 2.292±0.461. Conclusion: The results stated that the administration of Curcuma longa could reduce MDA levels, TNF-α, and IL-6 levels. Curcuma longa as an anti-inflammatory and anti-oxidant play an effective role in inhibiting inflammation by decreasing IL-6 cytokine and TNF-α. Curcuma longa can inhibit lipid peroxidation initiated by free radicals then reduce MDA levels.

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“…Negative control group consisted of 6 normal mice without treatment (CN), positive control group was for pregnant mice administered with carbon black treatment and performed surgery on day 18 (CP). In treatment 1,6 pregnant mice were exposed to carbon black with a dose of 1064 mg/m 3 for 8 hours per day at 6-17 days of gestational age and sacrificed on the 18 th day and given the CGA supplementation at 6-17 days of gestational age with a dose of 4.5 mg/kg body weight (T1) (Hendrawan et al, 2018). For the treatment 2,6 pregnant mice were exposed to carbon black with a dose of 1064 mg/m 3 for 8 hours per day, at 6-17 days of gestational age, sacrificed on the 18 th day, and given the CGA supplementation at 6-17 days of gestational age with a dose of 9 mg/kg body weight (T2).…”

Section: Population and Samplementioning

confidence: 99%

Chlorogenic Acid Therapy in Mice (Rattus norvegicus) Exposed to Carbon Black Towards p53 Expression on Placenta and Foetus Growth

Hendrawan¹,

Wulansari²,

Oktanella³

et al. 2020

Adv. Anim. Vet. Sci.

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This study aims to identify the expression of p53 on placenta and pregnant mice's foetus growth exposed by carbon black. The experimental units divided into five groups: negative control is pregnant mice without carbon black exposure and without Chlorogenic Acid therapy; positive control is pregnant mice exposed to carbon black without Chlorogenic Acid therapy; treatment 1, 2 and 3 were pregnant mice exposed to carbon black with Chlorogenic Acid therapy at 4.5 mg/kg body weight, 9 mg/kg body weight and 13.5 mg/kg body weight. The stages of the study began by mating female mice before exposing them to 1064 mg/m 3 carbon black for 8 hours, then administered with Chlorogenic Acid therapy. The detection of p53 expression was done with immunohistochemical staining and foetus growth with Alizarin red method observation. The results of the study show that chlorogenic acid administration has significant effects (p≤0.05) to the decrease in p53 placental expression compared to the positive control, hence the negative control (7.36 cm± 0.65 a), the positive control (32.00cm ± 0.21157 a), treatment 1 (25.00 cm± 0.3338 b), treatment 2 (16.93 cm± 0.39036 c) and treatment 3 (11.43cm ± 0.1397 d). There were significant differences in the length and weight of foetuses born between treatment groups (p≤0.05), hence the length of the foetus in the negative control (1.50 cm± 0.04 d), positive control (0.82 cm± 0.02a), treatment 1 (0.86 cm± 0.01 a), treatment 2 (1.01 cm± 0.98 b), and treatment 3 (1.44cm ± 0.03 cd). The foetal weight in the negative control is 4,082 g ± 0,131 c , in the positive control is 2,022 g ± 0,107 a , in the treatment 1 is 1,844 g ± 0,406 a , in the treatment 2 is 3,218 g ± 0,388 b , in the treatment 3 is 4,009 g ± 0.879 c. This study concludesthatthe administration of chlorogenic acid in pregnant mice exposed to carbon black can reduce the p53 expression on the placenta and also improve the placenta'slong and heavy performance.

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Effectiveness of chlorogenic acid supplementation on VEGF serum and placental MAP kinase expression in carbon Black-Exposed pregnant rattus norvegicus

Cited by 6 publications

References 0 publications

RANTES as a novel biomarker for atherogenic dyslipidemia and metabolic disturbances in patients with Type 2 Diabetes

RANTES as a novel biomarker for atherogenic dyslipidemia and metabolic disturbances in patients with Type 2 Diabetes

Curcuma longa supplementation reduce MDA, TNF-α, and IL-6 levels in a rat model exposed to soot particulates

Chlorogenic Acid Therapy in Mice (Rattus norvegicus) Exposed to Carbon Black Towards p53 Expression on Placenta and Foetus Growth

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